Abstract
We investigated the effect of different oxygen regimes on growth patterns of Pseudomonas spp. during benzene degradation in microcosm batch studies. Benzene degradation was induced by limiting oxygen available for microbial activity, which consists of three initial-dissolved oxygen (DO) levels of oxic, hypoxic, and anoxic conditions. Batch experiments were performed for cell growth and benzene degradation by inoculating three strains of Pseudomonas spp. (Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida) in mineral salt medium containing aqueous benzene. Results showed that all strains were capable to grow and degrade benzene under all oxygen regimes but in a different manner. The highest cell growth of P. aeruginosa and P. fluorescens was achieved under oxic and anoxic condition, respectively, but there was no substantial difference on benzene degradation between the oxygen treatments with about 25% reduction for both strains. P. putida showed a facultative process for both cell growth and benzene degradation. This reveals that care should be taken in selection of microorganisms with regard to environmental studies since they exhibit different responses for given environmental conditions such as DO levels.
| Original language | English |
|---|---|
| Pages (from-to) | 350-354 |
| Number of pages | 5 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 71 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2006 Jul 1 |
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ASJC Scopus subject areas
- Biotechnology
- Microbiology
- Bioengineering
- Microbiology (medical)
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Effect of dissolved oxygen regime on growth dynamics of Pseudomonas spp during benzene degradation. / Mahendran, B.; Choi, N. C.; Choi, J. W.; Kim, Dong Ju.
In: Applied Microbiology and Biotechnology, Vol. 71, No. 3, 01.07.2006, p. 350-354.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Effect of dissolved oxygen regime on growth dynamics of Pseudomonas spp during benzene degradation
AU - Mahendran, B.
AU - Choi, N. C.
AU - Choi, J. W.
AU - Kim, Dong Ju
PY - 2006/7/1
Y1 - 2006/7/1
N2 - We investigated the effect of different oxygen regimes on growth patterns of Pseudomonas spp. during benzene degradation in microcosm batch studies. Benzene degradation was induced by limiting oxygen available for microbial activity, which consists of three initial-dissolved oxygen (DO) levels of oxic, hypoxic, and anoxic conditions. Batch experiments were performed for cell growth and benzene degradation by inoculating three strains of Pseudomonas spp. (Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida) in mineral salt medium containing aqueous benzene. Results showed that all strains were capable to grow and degrade benzene under all oxygen regimes but in a different manner. The highest cell growth of P. aeruginosa and P. fluorescens was achieved under oxic and anoxic condition, respectively, but there was no substantial difference on benzene degradation between the oxygen treatments with about 25% reduction for both strains. P. putida showed a facultative process for both cell growth and benzene degradation. This reveals that care should be taken in selection of microorganisms with regard to environmental studies since they exhibit different responses for given environmental conditions such as DO levels.
AB - We investigated the effect of different oxygen regimes on growth patterns of Pseudomonas spp. during benzene degradation in microcosm batch studies. Benzene degradation was induced by limiting oxygen available for microbial activity, which consists of three initial-dissolved oxygen (DO) levels of oxic, hypoxic, and anoxic conditions. Batch experiments were performed for cell growth and benzene degradation by inoculating three strains of Pseudomonas spp. (Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida) in mineral salt medium containing aqueous benzene. Results showed that all strains were capable to grow and degrade benzene under all oxygen regimes but in a different manner. The highest cell growth of P. aeruginosa and P. fluorescens was achieved under oxic and anoxic condition, respectively, but there was no substantial difference on benzene degradation between the oxygen treatments with about 25% reduction for both strains. P. putida showed a facultative process for both cell growth and benzene degradation. This reveals that care should be taken in selection of microorganisms with regard to environmental studies since they exhibit different responses for given environmental conditions such as DO levels.
UR - http://www.scopus.com/inward/record.url?scp=33745514006&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745514006&partnerID=8YFLogxK
U2 - 10.1007/s00253-005-0152-1
DO - 10.1007/s00253-005-0152-1
M3 - Article
C2 - 16172886
AN - SCOPUS:33745514006
VL - 71
SP - 350
EP - 354
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 3
ER -