Effect of endogenous transglutaminase on threadfin bream surimi gelation

J. Yongsawatdigul, A. Worratao, Jae W. Park

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Transglutaminase (TGase) activity of threadfin bream mince was 99.6 units/g of dry weight. After washing and screw-pressed dewatering, 44% residual activity was retained. Covalent cross-linking of myosin heavy chain (MHC) was observed at both 25 and 40°C and supported by increased gel strength. When pre-incubation at 40 °C was prolonged to 4 h, breaking force and MHC decreased due to endogenous proteinase(s). TGase activity towards MHC and synthetic substrates was effectively inhibited by iodoacetic acid (IAA). Autolytic activity and degradation of MHC was inhibited by phenylmethanesulfonyl fluoride (PMSF). Addition of 0.2% Ca2+ significantly improved breaking force and increased MHC cross-linking of surimi gels pre-incubated at 40 °C for 2 h.

Original languageEnglish
Pages (from-to)3258-3263
Number of pages6
JournalJournal of Food Science
Volume67
Issue number9
Publication statusPublished - 2002 Nov 1
Externally publishedYes

Fingerprint

Polynemidae
surimi
Transglutaminases
Myosin Heavy Chains
myosin heavy chains
protein-glutamine gamma-glutamyltransferase
bream
gelation
crosslinking
Gels
iodoacetic acid
Iodoacetic Acid
Phenylmethylsulfonyl Fluoride
dewatering
gel strength
fluorides
screws
washing
Peptide Hydrolases
proteinases

ASJC Scopus subject areas

  • Food Science

Cite this

Yongsawatdigul, J., Worratao, A., & Park, J. W. (2002). Effect of endogenous transglutaminase on threadfin bream surimi gelation. Journal of Food Science, 67(9), 3258-3263.

Effect of endogenous transglutaminase on threadfin bream surimi gelation. / Yongsawatdigul, J.; Worratao, A.; Park, Jae W.

In: Journal of Food Science, Vol. 67, No. 9, 01.11.2002, p. 3258-3263.

Research output: Contribution to journalArticle

Yongsawatdigul, J, Worratao, A & Park, JW 2002, 'Effect of endogenous transglutaminase on threadfin bream surimi gelation', Journal of Food Science, vol. 67, no. 9, pp. 3258-3263.
Yongsawatdigul J, Worratao A, Park JW. Effect of endogenous transglutaminase on threadfin bream surimi gelation. Journal of Food Science. 2002 Nov 1;67(9):3258-3263.
Yongsawatdigul, J. ; Worratao, A. ; Park, Jae W. / Effect of endogenous transglutaminase on threadfin bream surimi gelation. In: Journal of Food Science. 2002 ; Vol. 67, No. 9. pp. 3258-3263.
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