Effect of GABAergic compounds on gonadotropin-releasing hormone receptor gene expression in the rat

Jae Young Seong, H. Jarry, S. Kuhnemuth, S. Leonhardt, W. Wuttke, K. Kim

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Using a competitive reverse transcription-polymerase chain reaction (RT- PCR), the amounts of GnRH receptor (GnRHR) transcript in a discrete nucleus micropunched from rat brain slices were determined. GnRHR transcript was highly expressed in anterior pituitary >> median eminence > posterior mediobasal hypothalamus (pMBH) > preoptic area (POA) but not in cortex and posterior pituitary, which were used as control tissues. To examine the effect of GABA on GnRHR transcript level, 10 nmol of muscimol, a GABA-A receptor agonist, or baclofen, a GABA-B receptor agonist, was microinjected into the lateral ventricle of ovariectomized rats. Two hours after an intraventricular injection, rats were decapitated. Blood was collected 1 h before and after drug administration and used for LH determination. Serum LH levels were significantly reduced by muscimol but not by baclofen within 2 h. The activation of GABA-A receptors with muscimol resulted in a significant inhibition in GnRHR transcript level in both the pMBH and POA but not in the pituitary. The activation of GABA-B receptors with baclofen, however, did not produce any effect on GnRHR transcript level in the pMBH and POA, as well as the pituitary. This experiment demonstrates for the first time that GABAergic neurotransmission, through GABA-A receptors, is involved in the regulation of GnRHR transcript level in the rat hypothalamus. This suggests that GABAergic neurotransmission regulates GnRHR gene expression in a coordinated, yet complex, fashion in the control of the neuroendocrine function of GnRH-LH axis.

Original languageEnglish
Pages (from-to)2587-2593
Number of pages7
JournalEndocrinology
Volume136
Issue number6
Publication statusPublished - 1995 Jan 1
Externally publishedYes

Fingerprint

LHRH Receptors
Gene Expression
Posterior Hypothalamus
Muscimol
Baclofen
Preoptic Area
GABA-A Receptors
Synaptic Transmission
GABA-B Receptor Agonists
Intraventricular Injections
GABA-B Receptors
GABA-A Receptor Agonists
Median Eminence
Lateral Ventricles
Gonadotropin-Releasing Hormone
gamma-Aminobutyric Acid
Hypothalamus
Reverse Transcription
Polymerase Chain Reaction
Brain

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Seong, J. Y., Jarry, H., Kuhnemuth, S., Leonhardt, S., Wuttke, W., & Kim, K. (1995). Effect of GABAergic compounds on gonadotropin-releasing hormone receptor gene expression in the rat. Endocrinology, 136(6), 2587-2593.

Effect of GABAergic compounds on gonadotropin-releasing hormone receptor gene expression in the rat. / Seong, Jae Young; Jarry, H.; Kuhnemuth, S.; Leonhardt, S.; Wuttke, W.; Kim, K.

In: Endocrinology, Vol. 136, No. 6, 01.01.1995, p. 2587-2593.

Research output: Contribution to journalArticle

Seong, JY, Jarry, H, Kuhnemuth, S, Leonhardt, S, Wuttke, W & Kim, K 1995, 'Effect of GABAergic compounds on gonadotropin-releasing hormone receptor gene expression in the rat', Endocrinology, vol. 136, no. 6, pp. 2587-2593.
Seong JY, Jarry H, Kuhnemuth S, Leonhardt S, Wuttke W, Kim K. Effect of GABAergic compounds on gonadotropin-releasing hormone receptor gene expression in the rat. Endocrinology. 1995 Jan 1;136(6):2587-2593.
Seong, Jae Young ; Jarry, H. ; Kuhnemuth, S. ; Leonhardt, S. ; Wuttke, W. ; Kim, K. / Effect of GABAergic compounds on gonadotropin-releasing hormone receptor gene expression in the rat. In: Endocrinology. 1995 ; Vol. 136, No. 6. pp. 2587-2593.
@article{77ee1597dbdd40d5aea7ee23c178dbb7,
title = "Effect of GABAergic compounds on gonadotropin-releasing hormone receptor gene expression in the rat",
abstract = "Using a competitive reverse transcription-polymerase chain reaction (RT- PCR), the amounts of GnRH receptor (GnRHR) transcript in a discrete nucleus micropunched from rat brain slices were determined. GnRHR transcript was highly expressed in anterior pituitary >> median eminence > posterior mediobasal hypothalamus (pMBH) > preoptic area (POA) but not in cortex and posterior pituitary, which were used as control tissues. To examine the effect of GABA on GnRHR transcript level, 10 nmol of muscimol, a GABA-A receptor agonist, or baclofen, a GABA-B receptor agonist, was microinjected into the lateral ventricle of ovariectomized rats. Two hours after an intraventricular injection, rats were decapitated. Blood was collected 1 h before and after drug administration and used for LH determination. Serum LH levels were significantly reduced by muscimol but not by baclofen within 2 h. The activation of GABA-A receptors with muscimol resulted in a significant inhibition in GnRHR transcript level in both the pMBH and POA but not in the pituitary. The activation of GABA-B receptors with baclofen, however, did not produce any effect on GnRHR transcript level in the pMBH and POA, as well as the pituitary. This experiment demonstrates for the first time that GABAergic neurotransmission, through GABA-A receptors, is involved in the regulation of GnRHR transcript level in the rat hypothalamus. This suggests that GABAergic neurotransmission regulates GnRHR gene expression in a coordinated, yet complex, fashion in the control of the neuroendocrine function of GnRH-LH axis.",
author = "Seong, {Jae Young} and H. Jarry and S. Kuhnemuth and S. Leonhardt and W. Wuttke and K. Kim",
year = "1995",
month = "1",
day = "1",
language = "English",
volume = "136",
pages = "2587--2593",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "6",

}

TY - JOUR

T1 - Effect of GABAergic compounds on gonadotropin-releasing hormone receptor gene expression in the rat

AU - Seong, Jae Young

AU - Jarry, H.

AU - Kuhnemuth, S.

AU - Leonhardt, S.

AU - Wuttke, W.

AU - Kim, K.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Using a competitive reverse transcription-polymerase chain reaction (RT- PCR), the amounts of GnRH receptor (GnRHR) transcript in a discrete nucleus micropunched from rat brain slices were determined. GnRHR transcript was highly expressed in anterior pituitary >> median eminence > posterior mediobasal hypothalamus (pMBH) > preoptic area (POA) but not in cortex and posterior pituitary, which were used as control tissues. To examine the effect of GABA on GnRHR transcript level, 10 nmol of muscimol, a GABA-A receptor agonist, or baclofen, a GABA-B receptor agonist, was microinjected into the lateral ventricle of ovariectomized rats. Two hours after an intraventricular injection, rats were decapitated. Blood was collected 1 h before and after drug administration and used for LH determination. Serum LH levels were significantly reduced by muscimol but not by baclofen within 2 h. The activation of GABA-A receptors with muscimol resulted in a significant inhibition in GnRHR transcript level in both the pMBH and POA but not in the pituitary. The activation of GABA-B receptors with baclofen, however, did not produce any effect on GnRHR transcript level in the pMBH and POA, as well as the pituitary. This experiment demonstrates for the first time that GABAergic neurotransmission, through GABA-A receptors, is involved in the regulation of GnRHR transcript level in the rat hypothalamus. This suggests that GABAergic neurotransmission regulates GnRHR gene expression in a coordinated, yet complex, fashion in the control of the neuroendocrine function of GnRH-LH axis.

AB - Using a competitive reverse transcription-polymerase chain reaction (RT- PCR), the amounts of GnRH receptor (GnRHR) transcript in a discrete nucleus micropunched from rat brain slices were determined. GnRHR transcript was highly expressed in anterior pituitary >> median eminence > posterior mediobasal hypothalamus (pMBH) > preoptic area (POA) but not in cortex and posterior pituitary, which were used as control tissues. To examine the effect of GABA on GnRHR transcript level, 10 nmol of muscimol, a GABA-A receptor agonist, or baclofen, a GABA-B receptor agonist, was microinjected into the lateral ventricle of ovariectomized rats. Two hours after an intraventricular injection, rats were decapitated. Blood was collected 1 h before and after drug administration and used for LH determination. Serum LH levels were significantly reduced by muscimol but not by baclofen within 2 h. The activation of GABA-A receptors with muscimol resulted in a significant inhibition in GnRHR transcript level in both the pMBH and POA but not in the pituitary. The activation of GABA-B receptors with baclofen, however, did not produce any effect on GnRHR transcript level in the pMBH and POA, as well as the pituitary. This experiment demonstrates for the first time that GABAergic neurotransmission, through GABA-A receptors, is involved in the regulation of GnRHR transcript level in the rat hypothalamus. This suggests that GABAergic neurotransmission regulates GnRHR gene expression in a coordinated, yet complex, fashion in the control of the neuroendocrine function of GnRH-LH axis.

UR - http://www.scopus.com/inward/record.url?scp=0029018615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029018615&partnerID=8YFLogxK

M3 - Article

VL - 136

SP - 2587

EP - 2593

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 6

ER -