Effect of gamma irradiation on physiological and proteomic changes of Arctic Zygnema sp. (Chlorophyta, Zygnematales)

Jong Il Choi, Minchul Yoon, Sangyong Lim, Gwang Hoon Kim, Hyun Jin Park

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this study, the effect of ionizing radiation on an Arctic alga was examined by assessing photosynthetic efficiency, antioxidant capacity, and proteomic changes. Arctic Zygnema sp. was gamma-irradiated at doses of 1, 3, and 5 kGy and showed serious cell damage after irradiation with the 5 kGy dose. The photosynthetic efficiency markedly decreased in gamma-irradiated Zygnema sp.; whereas, antioxidant capacity significantly increased. To investigate changes in protein expression levels, two-dimensional electrophoresis was performed. Thirty-nine protein spots were differently expressed. Several proteins, both upregulated and downregulated, were identified as photosynthesis-related proteins, confirming an important effect of gamma irradiation on the photosynthetic process. On the one hand, proteins similar to those involved in energy metabolism, isoprene biosynthesis, and protein biosynthesis were significantly downregulated. On the other hand, proteins related to DNA repair, quinone oxireductase, regulation of microtubules, and cell wall biogenesis were upregulated in gamma-irradiated Zygnema sp. These results provide insights on the effects of gamma irradiation on Zygnema sp. that can aid the interpretation of response mechanisms of Arctic algae to ionizing radiation.

Original languageEnglish
Pages (from-to)333-341
Number of pages9
JournalPhycologia
Volume54
Issue number4
DOIs
Publication statusPublished - 2015
Externally publishedYes

Fingerprint

Zygnema
Zygnematales
proteomics
gamma radiation
Chlorophyta
Arctic region
irradiation
algae
protein
ionizing radiation
proteins
protein synthesis
antioxidant
antioxidants
alga
dosage
DNA repair
quinones
energy metabolism
microtubules

Keywords

  • 2D-electrophoresis
  • Antioxidant
  • Arctic zygnema sp.
  • Gamma irradiation

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Effect of gamma irradiation on physiological and proteomic changes of Arctic Zygnema sp. (Chlorophyta, Zygnematales). / Choi, Jong Il; Yoon, Minchul; Lim, Sangyong; Kim, Gwang Hoon; Park, Hyun Jin.

In: Phycologia, Vol. 54, No. 4, 2015, p. 333-341.

Research output: Contribution to journalArticle

Choi, Jong Il ; Yoon, Minchul ; Lim, Sangyong ; Kim, Gwang Hoon ; Park, Hyun Jin. / Effect of gamma irradiation on physiological and proteomic changes of Arctic Zygnema sp. (Chlorophyta, Zygnematales). In: Phycologia. 2015 ; Vol. 54, No. 4. pp. 333-341.
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