Effect of ganglioside GT1b on the in vitro maturation of porcine oocytes and embryonic development

Seon Ung Hwang, Yubyeol Jeon, Junchul David Yoon, Lian Cai, Eunhye Kim, Hyunju Yoo, Kyu Jun Kim, Kyu Mi Park, Minghui Jin, Hyunggee Kim, Sang Hwan Hyun

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Ganglioside is an acidic glycosphingolipid with sialic acids residues. This study was performed to investigate the effect and mechanism of ganglioside GT1b in porcine oocytes in the process of in vitro maturation (IVM) and preimplantation development. Metaphase II (MII) rates were significantly (P <0.05) different between the control group and the 5 nM GT1b treatment group. Intracellular glutathione (GSH) levels in oocytes matured with 5 nM and 20 nM and GT1b decreased significantly (P <0.05). The 10 nM group showed a significant (P <0.05) decrease in intracellular reactive oxygen species (ROS) levels compared with the control group. Subsequently, the level of intracellular Ca2+ in oocytes treated with different concentrations of GT1b was measured. Intracellular Ca2+ was significantly (P <0.05) increased with a higher concentration of GT1b in a dose-dependent manner. Real-time PCR was performed and showed that the expression of bradykinin 2 receptor (B2R) and calcium/calmodulin-dependent protein kinase II delta (CaMKIIδ) in cumulus cells was significantly (P <0.05) decreased in the 20 nM GT1b treatment group. Treatment with 5 nM GT1b significantly (P <0.05) decreased the expression of CaMKIIδ. In oocytes, treatment with 5 nM GT1b significantly (P <0.05) decreased CaMKIIγ and POU5F1 (POU domain, class 5, transcription factor 1). However, treatment with 20 nM GT1b significantly (P <0.05) increased the expression of POU5F1. Finally, embryonic developmental data showed no significant differences in the two experiments (parthenogenesis and in vitro fertilization). In conclusion, the results of the present study indicated that GT1b plays an important role in increasing the nuclear maturation rate and decreasing the intracellular ROS levels during IVM. However, GT1b inhibited maturation of the cytoplasm by maintaining intracellular Ca2+ in the process of oocyte maturation regardless of the cell cycle stage. Therefore, GT1b is thought to act on another mechanism that controls intracellular Ca2+.

Original languageEnglish
Pages (from-to)549-557
Number of pages9
JournalJournal of Reproduction and Development
Volume61
Issue number6
DOIs
Publication statusPublished - 2015 Dec 18

Fingerprint

gangliosides
oocytes
embryogenesis
swine
calcium
calmodulin
protein kinases
reactive oxygen species
glycosphingolipids
bradykinin
sialic acids
parthenogenesis
in vitro fertilization
metaphase
glutathione
cell cycle
quantitative polymerase chain reaction
cytoplasm
transcription factors
receptors

Keywords

  • Embryonic development
  • Ganglioside GT1b
  • In vitro maturation (IVM)
  • Intracellular calcium
  • Porcine

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Effect of ganglioside GT1b on the in vitro maturation of porcine oocytes and embryonic development. / Hwang, Seon Ung; Jeon, Yubyeol; Yoon, Junchul David; Cai, Lian; Kim, Eunhye; Yoo, Hyunju; Kim, Kyu Jun; Park, Kyu Mi; Jin, Minghui; Kim, Hyunggee; Hyun, Sang Hwan.

In: Journal of Reproduction and Development, Vol. 61, No. 6, 18.12.2015, p. 549-557.

Research output: Contribution to journalArticle

Hwang, SU, Jeon, Y, Yoon, JD, Cai, L, Kim, E, Yoo, H, Kim, KJ, Park, KM, Jin, M, Kim, H & Hyun, SH 2015, 'Effect of ganglioside GT1b on the in vitro maturation of porcine oocytes and embryonic development', Journal of Reproduction and Development, vol. 61, no. 6, pp. 549-557. https://doi.org/10.1262/jrd.2015-049
Hwang, Seon Ung ; Jeon, Yubyeol ; Yoon, Junchul David ; Cai, Lian ; Kim, Eunhye ; Yoo, Hyunju ; Kim, Kyu Jun ; Park, Kyu Mi ; Jin, Minghui ; Kim, Hyunggee ; Hyun, Sang Hwan. / Effect of ganglioside GT1b on the in vitro maturation of porcine oocytes and embryonic development. In: Journal of Reproduction and Development. 2015 ; Vol. 61, No. 6. pp. 549-557.
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AU - Yoo, Hyunju

AU - Kim, Kyu Jun

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AU - Hyun, Sang Hwan

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AB - Ganglioside is an acidic glycosphingolipid with sialic acids residues. This study was performed to investigate the effect and mechanism of ganglioside GT1b in porcine oocytes in the process of in vitro maturation (IVM) and preimplantation development. Metaphase II (MII) rates were significantly (P <0.05) different between the control group and the 5 nM GT1b treatment group. Intracellular glutathione (GSH) levels in oocytes matured with 5 nM and 20 nM and GT1b decreased significantly (P <0.05). The 10 nM group showed a significant (P <0.05) decrease in intracellular reactive oxygen species (ROS) levels compared with the control group. Subsequently, the level of intracellular Ca2+ in oocytes treated with different concentrations of GT1b was measured. Intracellular Ca2+ was significantly (P <0.05) increased with a higher concentration of GT1b in a dose-dependent manner. Real-time PCR was performed and showed that the expression of bradykinin 2 receptor (B2R) and calcium/calmodulin-dependent protein kinase II delta (CaMKIIδ) in cumulus cells was significantly (P <0.05) decreased in the 20 nM GT1b treatment group. Treatment with 5 nM GT1b significantly (P <0.05) decreased the expression of CaMKIIδ. In oocytes, treatment with 5 nM GT1b significantly (P <0.05) decreased CaMKIIγ and POU5F1 (POU domain, class 5, transcription factor 1). However, treatment with 20 nM GT1b significantly (P <0.05) increased the expression of POU5F1. Finally, embryonic developmental data showed no significant differences in the two experiments (parthenogenesis and in vitro fertilization). In conclusion, the results of the present study indicated that GT1b plays an important role in increasing the nuclear maturation rate and decreasing the intracellular ROS levels during IVM. However, GT1b inhibited maturation of the cytoplasm by maintaining intracellular Ca2+ in the process of oocyte maturation regardless of the cell cycle stage. Therefore, GT1b is thought to act on another mechanism that controls intracellular Ca2+.

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