Effect of genistein on the expression of bone metabolism genes in ovariectomized mice using a cDNA microarray

Jae Eun Pie, Jin Hee Park, Yoon Hee Park, Yeon Mi Ryu, Ki Nam Kim, Seung Woo Suh, Kevin G. Becker, Yoon S. Cho-Chung, Meyoung Kon Kim

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

Osteoporosis associated with estrogen deficiency is defined as an abnormal decrease in bone mass leading to an increased fracture risk. Genistein (GEN), as a phytoestrogen, is a type of soybean-derived isoflavone that possesses structural similarity to estrogen. In this study, we assessed the effect of GEN in ovariectomized (OVX) mice. To determine the effect of GEN on bone metabolism, we investigated gene expression profiles using a radioactive cDNA microarray. Eight-week-old female mice were either sham operated (SHAM) or OVX. From 1 week after the operation, OVX mice were injected daily with intraperitoneal GEN (0.1, 0.5, 1.5 and 3.0 mg/day) or 17β-estradiol (E2, 0.03 μg/day) for 4 weeks. A cDNA microarray was used to evaluate changes in the expression of 1,152 genes. OVX mice showed bone mineral density (BMD) loss versus SHAM mice (5.8±0.4 vs. 6.9±0.6 mg/cm2). However, femur BMDs were completely restored by GEN and by E2 administration in OVX mice. Serum osteocalcin in OVX mice treated with 0.5 mg/day of GEN was 1.6-fold (44.30±5.73 ng/ml) higher than that in untreated mice. GEN treatment up-regulated 38 genes (e.g., mitogen-activated protein kinase 10) and down-regulated 18 (e.g., matrix metalloproteinase 13). Moreover, GEN was found to have a protective effect on bone loss caused by estrogen deficiency in OVX mice. The present study suggests that GEN modulates bone metabolism-related gene expression, including calciotropic receptor, cytokines, growth factors and bone matrix proteins.

Original languageEnglish
Pages (from-to)157-164
Number of pages8
JournalJournal of Nutritional Biochemistry
Volume17
Issue number3
DOIs
Publication statusPublished - 2006 Mar

Keywords

  • Bone metabolism
  • Gene profiling
  • Genistein
  • Osteoporosis
  • cDNA microarray

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

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