Effect of human bone marrow stromal cell allograft on proliferation and collagen synthesis of diabetic fibroblasts in vitro

Jeong Bae Kim, Kyung Wook Chun, Seung Kyu Han, Woo Kyung Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In previous pilot studies, the authors reported that bone marrow stromal cells (BSCs) have a superior effect on healing of acute wounds compared with fibroblasts in vitro and in vivo. The present study was designed to compare the effect of allograft of normal BSCs with that of normal fibroblasts on the activity of diabetic fibroblasts in vitro. Diabetic fibroblasts were cultured in 24-well culture plates. Three groups were evaluated: group I, diabetic fibroblasts only; group II, diabetic fibroblasts co-cultured with normal fibroblasts; group III, diabetic fibroblasts co-cultured with normal BSCs. After 3-day incubation, cell proliferation and collagen synthesis, which are the major contributing factors in wound healing, were measured. Group III showed the highest level in both cell proliferation and collagen synthesis, followed by group II and group I (p < 0.05). These results demonstrate that BSCs are superior to fibroblasts in stimulating the activity of diabetic fibroblasts.

Original languageEnglish
Pages (from-to)1030-1035
Number of pages6
JournalJournal of Plastic, Reconstructive and Aesthetic Surgery
Volume63
Issue number6
DOIs
Publication statusPublished - 2010 Jun

Keywords

  • Bone marrow stromal cell
  • Diabetic fibroblast

ASJC Scopus subject areas

  • Surgery

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