Effect of hydroxyapatite-tricalcium phosphate micro-particles on the cellular interaction of in situ hyaluronic acid-poly(ethylene oxide) hydrogel

Mi Sook Kim, Yoon Jeong Choi, Gun Woo Kim, Insup Noh, Yongdoo Park, Kyu Back Lee, In Sook Kim, Soon Jung Hwang

Research output: Contribution to journalArticle

Abstract

In vitro cell behaviors of calvarial osteoblasts (MC3T3) were evaluated by seeding them on both the surface and inside of in situ hyaluronic acid-poly(ethylene oxide) (HA-PEO) hydrogel, either after or before incorporation of mixture micro-particles of hydroxyapatite-β-tricalcium phosphate on/inside the hydrogel, respectively. Cellular behaviors such as adhesion and proliferation on the surface and inside the gel were evaluated with light microscopy and a microplate reader by focusing on the interactions of cell-HA-PEO as well as cell-hydroxyapatite-tricalcium phosphate micro-particle surface in the gel. Cell adhesion and spreading seemed to be enhanced by supplying the micro-particles to the inside the HA-PEO hydrogel, compared to the results of the HA-PEO hydrogel itself.

Original languageEnglish
Pages (from-to)765-768
Number of pages4
JournalKey Engineering Materials
Volume342-343
Publication statusPublished - 2007 Apr 16

Fingerprint

Hyaluronic acid
Hydrogel
Hyaluronic Acid
Durapatite
Polyethylene oxides
Hydroxyapatite
Hydrogels
Phosphates
Particles (particulate matter)
Gels
Osteoblasts
Cell adhesion
Optical microscopy
Adhesion
tricalcium phosphate

Keywords

  • Bone cell interaction
  • Hyaluronic acid
  • Hydroxyapatite
  • Poly(ethylene oxide)
  • Tricalcium phosphate

ASJC Scopus subject areas

  • Ceramics and Composites
  • Chemical Engineering (miscellaneous)

Cite this

Effect of hydroxyapatite-tricalcium phosphate micro-particles on the cellular interaction of in situ hyaluronic acid-poly(ethylene oxide) hydrogel. / Kim, Mi Sook; Choi, Yoon Jeong; Kim, Gun Woo; Noh, Insup; Park, Yongdoo; Lee, Kyu Back; Kim, In Sook; Hwang, Soon Jung.

In: Key Engineering Materials, Vol. 342-343, 16.04.2007, p. 765-768.

Research output: Contribution to journalArticle

Kim, Mi Sook ; Choi, Yoon Jeong ; Kim, Gun Woo ; Noh, Insup ; Park, Yongdoo ; Lee, Kyu Back ; Kim, In Sook ; Hwang, Soon Jung. / Effect of hydroxyapatite-tricalcium phosphate micro-particles on the cellular interaction of in situ hyaluronic acid-poly(ethylene oxide) hydrogel. In: Key Engineering Materials. 2007 ; Vol. 342-343. pp. 765-768.
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AU - Noh, Insup

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AU - Lee, Kyu Back

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