Effect of inoculum concentration on methanogenesis by direct interspecies electron transfer: Performance and microbial community composition

Hyun Jin Kang, Sang Hoon Lee, Tae Guen Lim, Hee-Deung Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

To understand the effect of inoculum concentration on direct interspecies electron transfer (DIET) for methanogenesis, batch-type anaerobic bioreactors with different inoculum concentrations were operated with and without supplemented granular activated carbon (GAC). With decrease in inoculum concentration, GAC-supplemented bioreactors showed faster methane production rates and reduced lag times. Geobacter species were specifically enriched on the GAC surfaces under lower inoculum concentration conditions. Together, the relative abundance of aceticlastic methanogens (competitors of Geobacter species for acetate) gradually decreased when the inoculum concentration increased. These results suggested that the specific enrichment of Geobacter species by outcompeting with aceticlastic methanogens through low inoculum concentrations on GAC surfaces accelerated methanogenesis by DIET via GAC in anaerobic bioreactors. Taken together, the results of this study suggested that inoculum concentration is an important factor in stimulating DIET for methane production.

Original languageEnglish
Article number121881
JournalBioresource Technology
Volume291
DOIs
Publication statusPublished - 2019 Nov 1

Fingerprint

methanogenesis
Activated carbon
activated carbon
community composition
microbial community
electron
Bioreactors
Electrons
bioreactor
Methanogens
Chemical analysis
Methane
methane
relative abundance
acetate
Acetates
effect

Keywords

  • Anaerobic digester
  • Direct interspecies electron transfer
  • Enrichment
  • Geobacter species
  • Inoculum concentration

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Effect of inoculum concentration on methanogenesis by direct interspecies electron transfer : Performance and microbial community composition. / Kang, Hyun Jin; Lee, Sang Hoon; Lim, Tae Guen; Park, Hee-Deung.

In: Bioresource Technology, Vol. 291, 121881, 01.11.2019.

Research output: Contribution to journalArticle

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