Effect of interleukin-18 gene polymorphisms on sensitization to wheat flour in bakery workers

Seung Hyun Kim, Gyu Young Hur, Hyun Jung Jin, Hyunna Choi, Hae Sim Park

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Lower respiratory symptoms in bakery workers may be induced by wheat flour and endotoxins. We hypothesized that endotoxins from wheat flour may stimulate innate immunity and that interleukin-18 (IL-18) gene polymorphisms may affect their regulatory role in innate immune responses to endotoxins. To investigate the genetic contribution of IL-18 to sensitization to wheat flour, we performed a genetic association study of IL-18 in Korean bakery workers. A total of 373 bakery workers undertook a questionnaire regarding work-related symptoms. Skin prick tests with common and occupational allergens were performed and specific antibodies to wheat flour were measured by ELISA. Three polymorphisms of the IL-18 gene (-607A/C, -137G/C, 8674C/G) were genotyped, and the functional effects of the polymorphisms were analyzed using the luciferase reporter assay. Genotypes of -137G/C (GC or CC) and haplotype ht3 [ACC] showed a significant association with the rate of sensitization to wheat flour. Luciferase activity assay indicated ht3 [AC] as a low transcript haplotype. In conclusion, the regulatory role of IL-18 in lipopolysaccharide-induced responses in bakery workers may be affected by this polymorphism, thus contributing to the development of sensitization to wheat flour and work-related respiratory symptoms.

    Original languageEnglish
    Pages (from-to)382-387
    Number of pages6
    JournalJournal of Korean medical science
    Volume27
    Issue number4
    DOIs
    Publication statusPublished - 2012 Apr

    Keywords

    • Genes
    • Interleukin-18
    • Polymorphism
    • Sensitization
    • Wheat flour

    ASJC Scopus subject areas

    • Medicine(all)

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