Effect of ionizing radiation on the DNA damage response in Chlamydomonas reinhardtii

Kwang Min Koo, Sera Jung, Jin Baek Kim, Sang Hoon Kim, Soon Jae Kwon, Won Joong Jeong, Gook Hyun Chung, Si Yong Kang, Yoon-E Choi, Joon Woo Ahn

Research output: Contribution to journalArticle

Abstract

The DNA damage response (DDR) is induced by various DNA damaging factors and maintains genome stability in all organisms. The Chlamydomonas reinhardtii genome contains putative homologous genes involved in DDR; however, little is known about the functions and responses of these genes to DNA damage. In this study, DDR by gamma radiation was determined in C. reinhardtii. Irradiation with 80, and 200�Gy gamma radiation caused death in approximately 47 and 97�% of C. reinhardtii cells, respectively. The absolute lethality of cells was at 300�Gy. The rate of DNA breaks was also determined using comet assays after exposure to different doses of gamma radiation. Irradiation with 80 and 400�Gy resulted in 17 and 34�% of nuclear degradation in C. reinhardtii cells, respectively. To identify the major DDR pathway of C. reinhardtii induced by gamma radiation, 24 putative DDR genes were selected from the Joint Genome Institute (JGI) database. Gamma radiation significantly affected expression of 15 genes among these. Therefore, these genes displaying expressional changes by gamma radiation are involved in DDR, which indicate that C. reinhardtii may possess a fundamental conserved DDR pathway with higher plants. Furthermore, radiation responsive proteins were identified by proteomic analysis, which are involved in metabolisms of carbohydrate, energy, and photosynthesis. This is the first report to describe the responses of DDR homologous genes to gamma radiation and to identify gamma radiation-responsive proteins in C. reinhardtii. Our data should provide molecular insights into gamma radiation responses including DNA damage in green algae.

Original languageEnglish
Pages (from-to)63-75
Number of pages13
JournalGenes and Genomics
Volume39
Issue number1
DOIs
Publication statusPublished - 2017 Jan 1

Fingerprint

Chlamydomonas reinhardtii
Ionizing radiation
Ionizing Radiation
Gamma Rays
DNA Damage
Gamma rays
Genes
DNA
Genome
Chlorophyta
DNA Breaks
Irradiation
Comet Assay
Genomic Instability
Carbohydrate Metabolism
Photosynthesis
Protein C
Proteomics
Energy Metabolism
Algae

Keywords

  • Chlamydomonas reinhardtii
  • DNA damage response (DDR)
  • DNA repair
  • Gamma radiation
  • Proteomic analysis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Koo, K. M., Jung, S., Kim, J. B., Kim, S. H., Kwon, S. J., Jeong, W. J., ... Ahn, J. W. (2017). Effect of ionizing radiation on the DNA damage response in Chlamydomonas reinhardtii. Genes and Genomics, 39(1), 63-75. https://doi.org/10.1007/s13258-016-0472-9

Effect of ionizing radiation on the DNA damage response in Chlamydomonas reinhardtii. / Koo, Kwang Min; Jung, Sera; Kim, Jin Baek; Kim, Sang Hoon; Kwon, Soon Jae; Jeong, Won Joong; Chung, Gook Hyun; Kang, Si Yong; Choi, Yoon-E; Ahn, Joon Woo.

In: Genes and Genomics, Vol. 39, No. 1, 01.01.2017, p. 63-75.

Research output: Contribution to journalArticle

Koo, KM, Jung, S, Kim, JB, Kim, SH, Kwon, SJ, Jeong, WJ, Chung, GH, Kang, SY, Choi, Y-E & Ahn, JW 2017, 'Effect of ionizing radiation on the DNA damage response in Chlamydomonas reinhardtii', Genes and Genomics, vol. 39, no. 1, pp. 63-75. https://doi.org/10.1007/s13258-016-0472-9
Koo, Kwang Min ; Jung, Sera ; Kim, Jin Baek ; Kim, Sang Hoon ; Kwon, Soon Jae ; Jeong, Won Joong ; Chung, Gook Hyun ; Kang, Si Yong ; Choi, Yoon-E ; Ahn, Joon Woo. / Effect of ionizing radiation on the DNA damage response in Chlamydomonas reinhardtii. In: Genes and Genomics. 2017 ; Vol. 39, No. 1. pp. 63-75.
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