Effect of lactoferrin-impregnated porous poly(lactide-co-glycolide) (PLGA) microspheres on osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs)

Sung Eun Kim, Young Pil Yun, Kyu Sik Shim, Kyeongsoon Park, Sung Wook Choi, Dong Hun Suh

    Research output: Contribution to journalArticlepeer-review

    36 Citations (Scopus)

    Abstract

    The aim of this study was to develop lactoferrin (LF)-impregnated porous poly(lactide-co-glycolide) (PLGA) microspheres (PMs) to induce osteogenic differentiation of rabbit adipose-derived stem cells (rADSCs). Porous PLGA PMs were fabricated by a fluidic device and their surfaces were modified with heparin-dopamine (Hep-DOPA). Then, LF (100. μg, 500. μg, and 1000. μg) was impregnated on the surface of heparinized PMs (Hep-PMs) via electrostatic interactions to yield LF-impregnated PMs. PMs and modified PMs were characterized by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). Osteogenic differentiation of rADSCs on PMs and modified PMs was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and mRNA expression of osteocalcin and osteopontin. Successful immobilization of Hep-DOPA and LF on the surface of PMs was confirmed by XPS analysis. LF-impregnated PMs generated significantly greater ALP activity, calcium deposition, and mRNA expression of osteocalcin and osteopontin compared with PMs. These results suggested that LF-impregnated PMs effectively induced osteogenic differentiation of rADSCs.

    Original languageEnglish
    Pages (from-to)457-464
    Number of pages8
    JournalColloids and Surfaces B: Biointerfaces
    Volume122
    DOIs
    Publication statusPublished - 2014 Oct 1

    Keywords

    • Fluidic device
    • Lactoferrin
    • Osteogenic differentiation
    • Porous microspheres
    • Rabbit adipose-derived stem cells

    ASJC Scopus subject areas

    • Biotechnology
    • Surfaces and Interfaces
    • Physical and Theoretical Chemistry
    • Colloid and Surface Chemistry

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