Effect of light conditions on mixotrophic cultivation of green microalgae

Anil Kumar Patel, Jae Min Joun, Min Eui Hong, Sang Jun Sim

Research output: Contribution to journalArticlepeer-review

93 Citations (Scopus)


Current research aimed to increase mixotrophic biomass from various organic carbon sources by exploring best light conditions. Three substrates glucose, acetic acid and glycerol were studied for their effects on mixotrophic microalgae cultivation under four light conditions. Light irradiance exhibited variability in growth response and photosynthetic efficiency based on type of substrates used in mixotrophic growth. Each substrate showed variability in light requirements for their effective assimilations. From growth responses, glucose and acetic acid respectively exhibited heterotrophic and mixotrophic (better growth in light) natures. Continuous light-deficient condition was adequate for effective mixotrophic growth as well as energy saving for glucose. However, light-sufficient condition required for effective acetic acid supported mixotrophic growth. Mixotrophic benefits from glycerol and its uptake by Chlorella protothecoides was negligible in all light conditions. Investigation of heterotrophic biomass contribution by various substrates in overall mixotrophic yield, glucose offered maximum approx. 43% contribution.

Original languageEnglish
Pages (from-to)245-253
Number of pages9
JournalBioresource technology
Publication statusPublished - 2019 Jun


  • Acetic acid
  • Autotrophic
  • Chlorella
  • Glucose
  • Glycerol
  • Microalgae
  • Mixotrophic

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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