Effect of Na-hyaluronan on stromal and endothelial healing in experimental corneal alkali wounds

Jang Hyun Chung, Yoo Kyung Park, Soo Min Paek, Young Hae Chong, Won-Ki Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The healing of stroma and endothelium after a standardized corneal alkali wound was evaluated in sodium-hyaluronan (Na-HA)-treated eyes. Alkali wounds were produced in one eye of each rabbit by applying a 5.5-mm round filter paper soaked in 1 N NaOH onto the central cornea for 60 s. Eyes were then treated with either 1% Na-HA (the treatment group) or phosphate-buffered saline (the control group) 4 times per day for 3 weeks. Endothelial wound morphometry was performed after alizarin red and trypan blue staining, while stromal healing was assessed by counting polymorphonuclear leukocytes (PMNs) and keratocytes in the central and marginal wound areas. During the early healing period the stroma treated with Na-HA had less PMNs than that of the control group. The size of the endothelial defect area measured 5 days after injury was significantly smaller in the Na-HA group than in the control corneas. The present findings indicate that topically applied 1% Na-HA affects stromal and endothelial healing during the early repair process after corneal alkali wounds.

Original languageEnglish
Pages (from-to)432-439
Number of pages8
JournalOphthalmic Research
Volume31
Issue number6
DOIs
Publication statusPublished - 1999 Nov 1
Externally publishedYes

Fingerprint

Alkalies
Hyaluronic Acid
Wounds and Injuries
Cornea
Control Groups
Trypan Blue
Endothelium
Neutrophils
Sodium
Phosphates
Staining and Labeling
Rabbits

Keywords

  • Alkali wounds
  • Cornea
  • Endothelium
  • Na-hyaluronan
  • Rabbit
  • Stroma

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Effect of Na-hyaluronan on stromal and endothelial healing in experimental corneal alkali wounds. / Chung, Jang Hyun; Park, Yoo Kyung; Paek, Soo Min; Chong, Young Hae; Kim, Won-Ki.

In: Ophthalmic Research, Vol. 31, No. 6, 01.11.1999, p. 432-439.

Research output: Contribution to journalArticle

Chung, Jang Hyun ; Park, Yoo Kyung ; Paek, Soo Min ; Chong, Young Hae ; Kim, Won-Ki. / Effect of Na-hyaluronan on stromal and endothelial healing in experimental corneal alkali wounds. In: Ophthalmic Research. 1999 ; Vol. 31, No. 6. pp. 432-439.
@article{28783bf23f3946319a2cdb129a484d5a,
title = "Effect of Na-hyaluronan on stromal and endothelial healing in experimental corneal alkali wounds",
abstract = "The healing of stroma and endothelium after a standardized corneal alkali wound was evaluated in sodium-hyaluronan (Na-HA)-treated eyes. Alkali wounds were produced in one eye of each rabbit by applying a 5.5-mm round filter paper soaked in 1 N NaOH onto the central cornea for 60 s. Eyes were then treated with either 1{\%} Na-HA (the treatment group) or phosphate-buffered saline (the control group) 4 times per day for 3 weeks. Endothelial wound morphometry was performed after alizarin red and trypan blue staining, while stromal healing was assessed by counting polymorphonuclear leukocytes (PMNs) and keratocytes in the central and marginal wound areas. During the early healing period the stroma treated with Na-HA had less PMNs than that of the control group. The size of the endothelial defect area measured 5 days after injury was significantly smaller in the Na-HA group than in the control corneas. The present findings indicate that topically applied 1{\%} Na-HA affects stromal and endothelial healing during the early repair process after corneal alkali wounds.",
keywords = "Alkali wounds, Cornea, Endothelium, Na-hyaluronan, Rabbit, Stroma",
author = "Chung, {Jang Hyun} and Park, {Yoo Kyung} and Paek, {Soo Min} and Chong, {Young Hae} and Won-Ki Kim",
year = "1999",
month = "11",
day = "1",
doi = "10.1159/000055568",
language = "English",
volume = "31",
pages = "432--439",
journal = "Ophthalmic Research",
issn = "0030-3747",
publisher = "S. Karger AG",
number = "6",

}

TY - JOUR

T1 - Effect of Na-hyaluronan on stromal and endothelial healing in experimental corneal alkali wounds

AU - Chung, Jang Hyun

AU - Park, Yoo Kyung

AU - Paek, Soo Min

AU - Chong, Young Hae

AU - Kim, Won-Ki

PY - 1999/11/1

Y1 - 1999/11/1

N2 - The healing of stroma and endothelium after a standardized corneal alkali wound was evaluated in sodium-hyaluronan (Na-HA)-treated eyes. Alkali wounds were produced in one eye of each rabbit by applying a 5.5-mm round filter paper soaked in 1 N NaOH onto the central cornea for 60 s. Eyes were then treated with either 1% Na-HA (the treatment group) or phosphate-buffered saline (the control group) 4 times per day for 3 weeks. Endothelial wound morphometry was performed after alizarin red and trypan blue staining, while stromal healing was assessed by counting polymorphonuclear leukocytes (PMNs) and keratocytes in the central and marginal wound areas. During the early healing period the stroma treated with Na-HA had less PMNs than that of the control group. The size of the endothelial defect area measured 5 days after injury was significantly smaller in the Na-HA group than in the control corneas. The present findings indicate that topically applied 1% Na-HA affects stromal and endothelial healing during the early repair process after corneal alkali wounds.

AB - The healing of stroma and endothelium after a standardized corneal alkali wound was evaluated in sodium-hyaluronan (Na-HA)-treated eyes. Alkali wounds were produced in one eye of each rabbit by applying a 5.5-mm round filter paper soaked in 1 N NaOH onto the central cornea for 60 s. Eyes were then treated with either 1% Na-HA (the treatment group) or phosphate-buffered saline (the control group) 4 times per day for 3 weeks. Endothelial wound morphometry was performed after alizarin red and trypan blue staining, while stromal healing was assessed by counting polymorphonuclear leukocytes (PMNs) and keratocytes in the central and marginal wound areas. During the early healing period the stroma treated with Na-HA had less PMNs than that of the control group. The size of the endothelial defect area measured 5 days after injury was significantly smaller in the Na-HA group than in the control corneas. The present findings indicate that topically applied 1% Na-HA affects stromal and endothelial healing during the early repair process after corneal alkali wounds.

KW - Alkali wounds

KW - Cornea

KW - Endothelium

KW - Na-hyaluronan

KW - Rabbit

KW - Stroma

UR - http://www.scopus.com/inward/record.url?scp=0032861035&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032861035&partnerID=8YFLogxK

U2 - 10.1159/000055568

DO - 10.1159/000055568

M3 - Article

VL - 31

SP - 432

EP - 439

JO - Ophthalmic Research

JF - Ophthalmic Research

SN - 0030-3747

IS - 6

ER -