Effect of optogenetic manipulation of accumbal medium spiny neurons expressing dopamine D2 receptors in cocaine-induced behavioral sensitization

Byeong Jun Kang, Shelly Sooyun Song, Lei Wen, Ki Pyo Hong, George J. Augustine, Ja-Hyun Baik

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Repetitive exposure to addictive drugs causes synaptic modification in the mesocorticolimbic dopamine (DA) system. Dopamine D1 receptors (D1R) or D2 receptors (D2R) expressed in the medium spiny neurons (MSNs) of the nucleus accumbens (NAc) play critical roles in the control of addictive behaviors. Optogenetic activation of D2R-expressing MSNs (D2R-MSNs) in the NAc previously demonstrated that these neurons play a key role in withdrawal-induced plasticity. Here, we examined the effect of optogenetic inhibition of D2R-MSNs in the NAc on cocaine-induced behavioral sensitization. Adeno-associated viral vectors encoding archaerhodopsin (ArchT) were delivered into the NAc of D2-Cre transgenic mice. Activation of ArchT produced photoinhibition of D2R-MSNs and caused disinhibition of neighboring MSNs in the NAc. However, such optogenetic silencing of D2R-MSNs in the NAc in vivo affected neither the initiation nor the expression of cocaine-induced behavioral sensitization. Similarly, photoinhibition of NAc D2R-MSNs in the NAc during the drug withdrawal period did not affect the expression of cocaine-induced behavioral sensitization. More detailed analysis of the effects of optogenetic activation of D2R-MSNs suggests that D2R-MSNs in the NAc exert important modulatory effects on neighboring MSN neurons, which may control the balanced output of NAc MSNs to control addictive behaviors.

Original languageEnglish
Pages (from-to)2056-2066
Number of pages11
JournalEuropean Journal of Neuroscience
Volume46
Issue number4
DOIs
Publication statusPublished - 2017 Aug 1

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Optogenetics
Dopamine D2 Receptors
Cocaine
Nucleus Accumbens
Neurons
Addictive Behavior
Dopamine D1 Receptors

Keywords

  • cocaine
  • dopamine D2 receptors
  • drug addiction
  • medium spiny neurons
  • optogenetics

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Effect of optogenetic manipulation of accumbal medium spiny neurons expressing dopamine D2 receptors in cocaine-induced behavioral sensitization. / Kang, Byeong Jun; Song, Shelly Sooyun; Wen, Lei; Hong, Ki Pyo; Augustine, George J.; Baik, Ja-Hyun.

In: European Journal of Neuroscience, Vol. 46, No. 4, 01.08.2017, p. 2056-2066.

Research output: Contribution to journalArticle

Kang, Byeong Jun ; Song, Shelly Sooyun ; Wen, Lei ; Hong, Ki Pyo ; Augustine, George J. ; Baik, Ja-Hyun. / Effect of optogenetic manipulation of accumbal medium spiny neurons expressing dopamine D2 receptors in cocaine-induced behavioral sensitization. In: European Journal of Neuroscience. 2017 ; Vol. 46, No. 4. pp. 2056-2066.
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