Effect of Panax ginseng on latency of passive avoidance response and neuronal damage of hippocampus

S. H. Cho, Sang-Hyun Choi, J. W. Choi, Dong-Hun Kim, Kyung-Ho Shin, Y. S. Chun, G. Chun B.-

Research output: Contribution to journalArticle

Abstract

The effects of crude saponin (SAP) and alkaloid (ALK) fractions of Panax ginseng C.A. Meyer on the detrimental effects of electroconvulsive shock (ECS) and scopolamine on passive avoidance response (PAR) were studied in male Sprague-Dawley rats, referring their effects on the neuronal injury and plasticity of hippocampus in response to electrolytic lesion of left entorhinal cortex (ECL). The detrimental ECS effect on PAR was attenuated bY pre- and post-treatments with SAP and ALK, respectively, or by pretreatment with aminoguanidine (AG), an inhibitor of diamine oxidase and NO synthase. And the detrimental scopolamine effect on PAR was also inhibited by pre- treatment with ALK or AG, and by post-treatment with SAP or ALK, respectively. On the 7th day after ECL, the brain sections stained by cresyl violet and by acetylcholinesterase (ACHE) histochemistry, respectively, showed the chromatolysis and numeral decrease of neurons and the reduction of ACHE reactivity in the hippocampus CA1 area and to a lesser extent, in the dentate gyrus. The neuronal cell death of the CA1 area was significantly reduced by SAP, ALK, or AG, and the reduction of AChE reactivity was significantly attenuated by SAP or ALK and to a lesser extent by AG. These results suggests that the protective effect of ginseng SAP and ALK fractions on ECS- or scopolamine-induced impairment of PAR may be ascribed in part to preservation of hippocampal neurons, particularly cholinergic neurons.

Original languageEnglish
Pages (from-to)345-353
Number of pages9
JournalKorean Journal of Physiology and Pharmacology
Volume1
Issue number4
Publication statusPublished - 1997 Dec 1

Fingerprint

Panax
Alkaloids
Saponins
Hippocampus
Electroshock
Scopolamine Hydrobromide
Acetylcholinesterase
Amine Oxidase (Copper-Containing)
Neurons
Entorhinal Cortex
Neuronal Plasticity
Cholinergic Neurons
Dentate Gyrus
Nitric Oxide Synthase
Sprague Dawley Rats
Cell Death
pimagedine
Wounds and Injuries
Brain

Keywords

  • Amino-guanidine
  • Cholinesterase
  • ECS
  • Entorhinal cortex lesion
  • Ginseng
  • Hippocampus
  • Passive avoidance response
  • Scopolamine

ASJC Scopus subject areas

  • Pharmacology
  • Physiology

Cite this

Effect of Panax ginseng on latency of passive avoidance response and neuronal damage of hippocampus. / Cho, S. H.; Choi, Sang-Hyun; Choi, J. W.; Kim, Dong-Hun; Shin, Kyung-Ho; Chun, Y. S.; Chun B.-, G.

In: Korean Journal of Physiology and Pharmacology, Vol. 1, No. 4, 01.12.1997, p. 345-353.

Research output: Contribution to journalArticle

@article{961a1a304a5740a193bdcef32dd2c51a,
title = "Effect of Panax ginseng on latency of passive avoidance response and neuronal damage of hippocampus",
abstract = "The effects of crude saponin (SAP) and alkaloid (ALK) fractions of Panax ginseng C.A. Meyer on the detrimental effects of electroconvulsive shock (ECS) and scopolamine on passive avoidance response (PAR) were studied in male Sprague-Dawley rats, referring their effects on the neuronal injury and plasticity of hippocampus in response to electrolytic lesion of left entorhinal cortex (ECL). The detrimental ECS effect on PAR was attenuated bY pre- and post-treatments with SAP and ALK, respectively, or by pretreatment with aminoguanidine (AG), an inhibitor of diamine oxidase and NO synthase. And the detrimental scopolamine effect on PAR was also inhibited by pre- treatment with ALK or AG, and by post-treatment with SAP or ALK, respectively. On the 7th day after ECL, the brain sections stained by cresyl violet and by acetylcholinesterase (ACHE) histochemistry, respectively, showed the chromatolysis and numeral decrease of neurons and the reduction of ACHE reactivity in the hippocampus CA1 area and to a lesser extent, in the dentate gyrus. The neuronal cell death of the CA1 area was significantly reduced by SAP, ALK, or AG, and the reduction of AChE reactivity was significantly attenuated by SAP or ALK and to a lesser extent by AG. These results suggests that the protective effect of ginseng SAP and ALK fractions on ECS- or scopolamine-induced impairment of PAR may be ascribed in part to preservation of hippocampal neurons, particularly cholinergic neurons.",
keywords = "Amino-guanidine, Cholinesterase, ECS, Entorhinal cortex lesion, Ginseng, Hippocampus, Passive avoidance response, Scopolamine",
author = "Cho, {S. H.} and Sang-Hyun Choi and Choi, {J. W.} and Dong-Hun Kim and Kyung-Ho Shin and Chun, {Y. S.} and {Chun B.-}, G.",
year = "1997",
month = "12",
day = "1",
language = "English",
volume = "1",
pages = "345--353",
journal = "Korean Journal of Physiology and Pharmacology",
issn = "1226-4512",
publisher = "Korean Physiological Soc. and Korean Soc. of Pharmacology",
number = "4",

}

TY - JOUR

T1 - Effect of Panax ginseng on latency of passive avoidance response and neuronal damage of hippocampus

AU - Cho, S. H.

AU - Choi, Sang-Hyun

AU - Choi, J. W.

AU - Kim, Dong-Hun

AU - Shin, Kyung-Ho

AU - Chun, Y. S.

AU - Chun B.-, G.

PY - 1997/12/1

Y1 - 1997/12/1

N2 - The effects of crude saponin (SAP) and alkaloid (ALK) fractions of Panax ginseng C.A. Meyer on the detrimental effects of electroconvulsive shock (ECS) and scopolamine on passive avoidance response (PAR) were studied in male Sprague-Dawley rats, referring their effects on the neuronal injury and plasticity of hippocampus in response to electrolytic lesion of left entorhinal cortex (ECL). The detrimental ECS effect on PAR was attenuated bY pre- and post-treatments with SAP and ALK, respectively, or by pretreatment with aminoguanidine (AG), an inhibitor of diamine oxidase and NO synthase. And the detrimental scopolamine effect on PAR was also inhibited by pre- treatment with ALK or AG, and by post-treatment with SAP or ALK, respectively. On the 7th day after ECL, the brain sections stained by cresyl violet and by acetylcholinesterase (ACHE) histochemistry, respectively, showed the chromatolysis and numeral decrease of neurons and the reduction of ACHE reactivity in the hippocampus CA1 area and to a lesser extent, in the dentate gyrus. The neuronal cell death of the CA1 area was significantly reduced by SAP, ALK, or AG, and the reduction of AChE reactivity was significantly attenuated by SAP or ALK and to a lesser extent by AG. These results suggests that the protective effect of ginseng SAP and ALK fractions on ECS- or scopolamine-induced impairment of PAR may be ascribed in part to preservation of hippocampal neurons, particularly cholinergic neurons.

AB - The effects of crude saponin (SAP) and alkaloid (ALK) fractions of Panax ginseng C.A. Meyer on the detrimental effects of electroconvulsive shock (ECS) and scopolamine on passive avoidance response (PAR) were studied in male Sprague-Dawley rats, referring their effects on the neuronal injury and plasticity of hippocampus in response to electrolytic lesion of left entorhinal cortex (ECL). The detrimental ECS effect on PAR was attenuated bY pre- and post-treatments with SAP and ALK, respectively, or by pretreatment with aminoguanidine (AG), an inhibitor of diamine oxidase and NO synthase. And the detrimental scopolamine effect on PAR was also inhibited by pre- treatment with ALK or AG, and by post-treatment with SAP or ALK, respectively. On the 7th day after ECL, the brain sections stained by cresyl violet and by acetylcholinesterase (ACHE) histochemistry, respectively, showed the chromatolysis and numeral decrease of neurons and the reduction of ACHE reactivity in the hippocampus CA1 area and to a lesser extent, in the dentate gyrus. The neuronal cell death of the CA1 area was significantly reduced by SAP, ALK, or AG, and the reduction of AChE reactivity was significantly attenuated by SAP or ALK and to a lesser extent by AG. These results suggests that the protective effect of ginseng SAP and ALK fractions on ECS- or scopolamine-induced impairment of PAR may be ascribed in part to preservation of hippocampal neurons, particularly cholinergic neurons.

KW - Amino-guanidine

KW - Cholinesterase

KW - ECS

KW - Entorhinal cortex lesion

KW - Ginseng

KW - Hippocampus

KW - Passive avoidance response

KW - Scopolamine

UR - http://www.scopus.com/inward/record.url?scp=0030725432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030725432&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0030725432

VL - 1

SP - 345

EP - 353

JO - Korean Journal of Physiology and Pharmacology

JF - Korean Journal of Physiology and Pharmacology

SN - 1226-4512

IS - 4

ER -