Effect of particulate matter on the NLRP3 inflammasomes in ocular tissues and cervical lymph nodes

Su Yeon Kang, Boram Kang, Hyo Myung Kim, Jong-Suk Song

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: To evaluate changes of the NLRP3 inflammasome complex and proinflammatory cytokine interleukin (IL)-1β in ocular tissues and cervical lymph nodes when the ocular surface was exposed to particulate matter. Methods: Five-week-old Sprague Dawley male rats, each weighing 250–300 g, were used in this study. The rats were randomly divided into two groups involving the TiO2-exposed group (n = 12) and control group (n = 12). Rats in the TiO2-exposed group were exposed to airborne TiO2 microparticles in the exposure chamber twice daily for 2 hours for 5 days. The mean particulate matter (PM) 10 concentration in the exposure chamber was 331 ± 83 µg/m3. The score of corneal fluorescein staining for evaluating ocular surface damage and the size of cervical lymph nodes for evaluating the immune response in the lymphatic drainage pathway were measured. The expression of the nucleotide-binding domain leucine-rich repeats, pyrin domain-containing 3 (NLRP3) inflammasome complex (NLRP3, apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (CARD) domain [ASC], and caspase-1) and IL-1β was quantitatively evaluated. Results: In the TiO2-exposed group, the mean corneal stain score was significantly increased compared to that of the control group (p = 0.002), and the size of the cervical lymph nodes in the TiO2-exposed group was greater than that of the control group (p = 0.004). The expression of NLRP3 and ASC was significantly increased in the corneo-conjunctival tissue and cervical lymph nodes after TiO2 exposure (both, p = 0.037). Inactive precursor forms of caspase-1 and IL-1β were decreased, but active forms of caspase-1 and IL-1β were increased after TiO2 exposure (both, p = 0.037). Conclusions: When the ocular surface was exposed to ambient particulate matter, the signal pathway of the NLRP3 inflammasome complex was activated, resulting in increased expression of the proinflammatory cytokine, IL-1β. The results suggested that the NLRP3 inflammasome complex was involved in the pathogenesis of ocular surface inflammation caused by exposure to particulate matter.

Original languageEnglish
Pages (from-to)130-136
Number of pages7
JournalJournal of Korean Ophthalmological Society
Volume59
Issue number2
DOIs
Publication statusPublished - 2018 Feb 1

Fingerprint

Inflammasomes
Particulate Matter
Interleukin-1
Caspase 1
Lymph Nodes
Control Groups
Cytokines
Fluorescein
Leucine
Sprague Dawley Rats
Drainage
Signal Transduction
Coloring Agents
Nucleotides
Apoptosis
Staining and Labeling
Inflammation
Proteins

Keywords

  • Dry eye
  • IL-1β
  • NLRP3 inflammasome
  • Particulate matter

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Effect of particulate matter on the NLRP3 inflammasomes in ocular tissues and cervical lymph nodes. / Kang, Su Yeon; Kang, Boram; Kim, Hyo Myung; Song, Jong-Suk.

In: Journal of Korean Ophthalmological Society, Vol. 59, No. 2, 01.02.2018, p. 130-136.

Research output: Contribution to journalArticle

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abstract = "Purpose: To evaluate changes of the NLRP3 inflammasome complex and proinflammatory cytokine interleukin (IL)-1β in ocular tissues and cervical lymph nodes when the ocular surface was exposed to particulate matter. Methods: Five-week-old Sprague Dawley male rats, each weighing 250–300 g, were used in this study. The rats were randomly divided into two groups involving the TiO2-exposed group (n = 12) and control group (n = 12). Rats in the TiO2-exposed group were exposed to airborne TiO2 microparticles in the exposure chamber twice daily for 2 hours for 5 days. The mean particulate matter (PM) 10 concentration in the exposure chamber was 331 ± 83 µg/m3. The score of corneal fluorescein staining for evaluating ocular surface damage and the size of cervical lymph nodes for evaluating the immune response in the lymphatic drainage pathway were measured. The expression of the nucleotide-binding domain leucine-rich repeats, pyrin domain-containing 3 (NLRP3) inflammasome complex (NLRP3, apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (CARD) domain [ASC], and caspase-1) and IL-1β was quantitatively evaluated. Results: In the TiO2-exposed group, the mean corneal stain score was significantly increased compared to that of the control group (p = 0.002), and the size of the cervical lymph nodes in the TiO2-exposed group was greater than that of the control group (p = 0.004). The expression of NLRP3 and ASC was significantly increased in the corneo-conjunctival tissue and cervical lymph nodes after TiO2 exposure (both, p = 0.037). Inactive precursor forms of caspase-1 and IL-1β were decreased, but active forms of caspase-1 and IL-1β were increased after TiO2 exposure (both, p = 0.037). Conclusions: When the ocular surface was exposed to ambient particulate matter, the signal pathway of the NLRP3 inflammasome complex was activated, resulting in increased expression of the proinflammatory cytokine, IL-1β. The results suggested that the NLRP3 inflammasome complex was involved in the pathogenesis of ocular surface inflammation caused by exposure to particulate matter.",
keywords = "Dry eye, IL-1β, NLRP3 inflammasome, Particulate matter",
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AU - Kang, Su Yeon

AU - Kang, Boram

AU - Kim, Hyo Myung

AU - Song, Jong-Suk

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Purpose: To evaluate changes of the NLRP3 inflammasome complex and proinflammatory cytokine interleukin (IL)-1β in ocular tissues and cervical lymph nodes when the ocular surface was exposed to particulate matter. Methods: Five-week-old Sprague Dawley male rats, each weighing 250–300 g, were used in this study. The rats were randomly divided into two groups involving the TiO2-exposed group (n = 12) and control group (n = 12). Rats in the TiO2-exposed group were exposed to airborne TiO2 microparticles in the exposure chamber twice daily for 2 hours for 5 days. The mean particulate matter (PM) 10 concentration in the exposure chamber was 331 ± 83 µg/m3. The score of corneal fluorescein staining for evaluating ocular surface damage and the size of cervical lymph nodes for evaluating the immune response in the lymphatic drainage pathway were measured. The expression of the nucleotide-binding domain leucine-rich repeats, pyrin domain-containing 3 (NLRP3) inflammasome complex (NLRP3, apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (CARD) domain [ASC], and caspase-1) and IL-1β was quantitatively evaluated. Results: In the TiO2-exposed group, the mean corneal stain score was significantly increased compared to that of the control group (p = 0.002), and the size of the cervical lymph nodes in the TiO2-exposed group was greater than that of the control group (p = 0.004). The expression of NLRP3 and ASC was significantly increased in the corneo-conjunctival tissue and cervical lymph nodes after TiO2 exposure (both, p = 0.037). Inactive precursor forms of caspase-1 and IL-1β were decreased, but active forms of caspase-1 and IL-1β were increased after TiO2 exposure (both, p = 0.037). Conclusions: When the ocular surface was exposed to ambient particulate matter, the signal pathway of the NLRP3 inflammasome complex was activated, resulting in increased expression of the proinflammatory cytokine, IL-1β. The results suggested that the NLRP3 inflammasome complex was involved in the pathogenesis of ocular surface inflammation caused by exposure to particulate matter.

AB - Purpose: To evaluate changes of the NLRP3 inflammasome complex and proinflammatory cytokine interleukin (IL)-1β in ocular tissues and cervical lymph nodes when the ocular surface was exposed to particulate matter. Methods: Five-week-old Sprague Dawley male rats, each weighing 250–300 g, were used in this study. The rats were randomly divided into two groups involving the TiO2-exposed group (n = 12) and control group (n = 12). Rats in the TiO2-exposed group were exposed to airborne TiO2 microparticles in the exposure chamber twice daily for 2 hours for 5 days. The mean particulate matter (PM) 10 concentration in the exposure chamber was 331 ± 83 µg/m3. The score of corneal fluorescein staining for evaluating ocular surface damage and the size of cervical lymph nodes for evaluating the immune response in the lymphatic drainage pathway were measured. The expression of the nucleotide-binding domain leucine-rich repeats, pyrin domain-containing 3 (NLRP3) inflammasome complex (NLRP3, apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (CARD) domain [ASC], and caspase-1) and IL-1β was quantitatively evaluated. Results: In the TiO2-exposed group, the mean corneal stain score was significantly increased compared to that of the control group (p = 0.002), and the size of the cervical lymph nodes in the TiO2-exposed group was greater than that of the control group (p = 0.004). The expression of NLRP3 and ASC was significantly increased in the corneo-conjunctival tissue and cervical lymph nodes after TiO2 exposure (both, p = 0.037). Inactive precursor forms of caspase-1 and IL-1β were decreased, but active forms of caspase-1 and IL-1β were increased after TiO2 exposure (both, p = 0.037). Conclusions: When the ocular surface was exposed to ambient particulate matter, the signal pathway of the NLRP3 inflammasome complex was activated, resulting in increased expression of the proinflammatory cytokine, IL-1β. The results suggested that the NLRP3 inflammasome complex was involved in the pathogenesis of ocular surface inflammation caused by exposure to particulate matter.

KW - Dry eye

KW - IL-1β

KW - NLRP3 inflammasome

KW - Particulate matter

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