Effect of PEG-PLLA diblock copolymer on macroporous PLLA scaffolds by thermally induced phase separation

Hyun Do Kim, Eun Hee Bae, Ick Chan Kwon, Ravindra Ramsurat Pal, Jae Do Nam, Doo Sung Lee

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

A regular and highly interconnected macroporous poly(L-lactic acid) (PLLA) scaffold was fabricated from a PLLA-dioxane-water ternary system with added polyethylene glycol (PEG)-PLLA diblock using thermally induced phase separation (TIPS). The morphology of the scaffold was investigated in detail by controlling the following TIPS parameters: quenching temperature, aging time, polymer concentration, molecular structure, and diblock concentration. The phase diagram was assessed visually on the basis of the turbidity. The cloud-point curve shifted to higher temperatures with increasing PEG content in the additives (PEG-PLLA diblocks), due to a stronger interaction between PEG and water in solution. The addition of diblock series (0.5wt% in solution) stabilized interconnections of pores at a later stage without segregation or sedimentation. The pore size of the scaffold could be easily controlled in the range 50-300μm. A macroporous PLLA scaffold was used to study an MC3T3-E1 cell (an osteoblast-like cell) culture. The cells successfully proliferated in the PLLA scaffold in the presence of added PEG-PLLA diblock for 4 weeks.

Original languageEnglish
Pages (from-to)2319-2329
Number of pages11
JournalBiomaterials
Volume25
Issue number12
DOIs
Publication statusPublished - 2004 May 1
Externally publishedYes

Fingerprint

Lactic acid
Scaffolds
Phase separation
Polyethylene glycols
Block copolymers
Temperature
Water
Osteoblasts
Turbidity
Ternary systems
poly(lactic acid)
Molecular Structure
Sedimentation
Cell culture
Molecular structure
Pore size
Phase diagrams
Quenching
Polymers
Cell Culture Techniques

Keywords

  • Macroporous scaffold
  • MC3T3-E1 cell
  • PEG-PLLA diblock copolymer
  • PLLA
  • Thermally induced phase separation (TIPS)

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Effect of PEG-PLLA diblock copolymer on macroporous PLLA scaffolds by thermally induced phase separation. / Kim, Hyun Do; Bae, Eun Hee; Kwon, Ick Chan; Pal, Ravindra Ramsurat; Nam, Jae Do; Lee, Doo Sung.

In: Biomaterials, Vol. 25, No. 12, 01.05.2004, p. 2319-2329.

Research output: Contribution to journalArticle

Kim, Hyun Do ; Bae, Eun Hee ; Kwon, Ick Chan ; Pal, Ravindra Ramsurat ; Nam, Jae Do ; Lee, Doo Sung. / Effect of PEG-PLLA diblock copolymer on macroporous PLLA scaffolds by thermally induced phase separation. In: Biomaterials. 2004 ; Vol. 25, No. 12. pp. 2319-2329.
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