Effect of peroxisome proliferator-activated receptor gamma agonists on myofibroblast differentiation and collagen production in nasal polyp-derived fibroblasts

Heung Man Lee; Hee Jun Kang; Hyo Hun Park; Seok Chan Hong; Jin Kook Kim; Jae Hoon Cho

doi:10.1177/000348940911801007

Effect of peroxisome proliferator-activated receptor gamma agonists on myofibroblast differentiation and collagen production in nasal polyp-derived fibroblasts

Heung Man Lee, Hee Jun Kang, Hyo Hun Park, Seok Chan Hong, Jin Kook Kim, Jae Hoon Cho

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Objectives: We investigated the effect of transforming growth factor-β1 (TGF-β1) on the differentiation of nasal polyp-derived fibroblasts (NPDFs) and on their production of collagen, and investigated the effect of peroxisome proliferator-activated receptor γ (PPARγ) agonists on the effects of TGF-β1. Methods: Primary fibroblast lines were established from 5 human nasal polyp tissues. We activated the NPDFs with TGF-β1 and exposed them to the PPARγ agonists ciglitazone and 15-deoxy-Δ-prostaglandin J2. The expression of α-smooth muscle actin (α-SMA) and collagen was measured by reverse transcriptase-polymerase chain reactions, immunocytochemical staining, and collagen assays. Results: The treatment with TGF-β1 increased the expression of α-SMA and collagen, and expression was markedly attenuated by the PPARγ agonists. Conclusions: PPARγ agonists inhibit the differentiation of TGF-β1-activated NPDFs and their production of collagen.

Original language	English
Pages (from-to)	721-727
Number of pages	7
Journal	Annals of Otology, Rhinology and Laryngology
Volume	118
Issue number	10
DOIs	https://doi.org/10.1177/000348940911801007
Publication status	Published - 2009 Oct

Keywords

Fibroblast
Nasal polyp
Peroxisome proliferator-activated receptor gamma agonist

ASJC Scopus subject areas

Otorhinolaryngology

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Effect of peroxisome proliferator-activated receptor gamma agonists on myofibroblast differentiation and collagen production in nasal polyp-derived fibroblasts. / Lee, Heung Man; Kang, Hee Jun; Park, Hyo Hun; Hong, Seok Chan; Kim, Jin Kook; Cho, Jae Hoon.

In: Annals of Otology, Rhinology and Laryngology, Vol. 118, No. 10, 10.2009, p. 721-727.

Research output: Contribution to journal › Article › peer-review

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abstract = "Objectives: We investigated the effect of transforming growth factor-β1 (TGF-β1) on the differentiation of nasal polyp-derived fibroblasts (NPDFs) and on their production of collagen, and investigated the effect of peroxisome proliferator-activated receptor γ (PPARγ) agonists on the effects of TGF-β1. Methods: Primary fibroblast lines were established from 5 human nasal polyp tissues. We activated the NPDFs with TGF-β1 and exposed them to the PPARγ agonists ciglitazone and 15-deoxy-Δ-prostaglandin J2. The expression of α-smooth muscle actin (α-SMA) and collagen was measured by reverse transcriptase-polymerase chain reactions, immunocytochemical staining, and collagen assays. Results: The treatment with TGF-β1 increased the expression of α-SMA and collagen, and expression was markedly attenuated by the PPARγ agonists. Conclusions: PPARγ agonists inhibit the differentiation of TGF-β1-activated NPDFs and their production of collagen.",

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AU - Lee, Heung Man

AU - Kang, Hee Jun

AU - Park, Hyo Hun

AU - Hong, Seok Chan

AU - Kim, Jin Kook

AU - Cho, Jae Hoon

PY - 2009/10

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N2 - Objectives: We investigated the effect of transforming growth factor-β1 (TGF-β1) on the differentiation of nasal polyp-derived fibroblasts (NPDFs) and on their production of collagen, and investigated the effect of peroxisome proliferator-activated receptor γ (PPARγ) agonists on the effects of TGF-β1. Methods: Primary fibroblast lines were established from 5 human nasal polyp tissues. We activated the NPDFs with TGF-β1 and exposed them to the PPARγ agonists ciglitazone and 15-deoxy-Δ-prostaglandin J2. The expression of α-smooth muscle actin (α-SMA) and collagen was measured by reverse transcriptase-polymerase chain reactions, immunocytochemical staining, and collagen assays. Results: The treatment with TGF-β1 increased the expression of α-SMA and collagen, and expression was markedly attenuated by the PPARγ agonists. Conclusions: PPARγ agonists inhibit the differentiation of TGF-β1-activated NPDFs and their production of collagen.

AB - Objectives: We investigated the effect of transforming growth factor-β1 (TGF-β1) on the differentiation of nasal polyp-derived fibroblasts (NPDFs) and on their production of collagen, and investigated the effect of peroxisome proliferator-activated receptor γ (PPARγ) agonists on the effects of TGF-β1. Methods: Primary fibroblast lines were established from 5 human nasal polyp tissues. We activated the NPDFs with TGF-β1 and exposed them to the PPARγ agonists ciglitazone and 15-deoxy-Δ-prostaglandin J2. The expression of α-smooth muscle actin (α-SMA) and collagen was measured by reverse transcriptase-polymerase chain reactions, immunocytochemical staining, and collagen assays. Results: The treatment with TGF-β1 increased the expression of α-SMA and collagen, and expression was markedly attenuated by the PPARγ agonists. Conclusions: PPARγ agonists inhibit the differentiation of TGF-β1-activated NPDFs and their production of collagen.

KW - Fibroblast

KW - Nasal polyp

KW - Peroxisome proliferator-activated receptor gamma agonist

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Effect of peroxisome proliferator-activated receptor gamma agonists on myofibroblast differentiation and collagen production in nasal polyp-derived fibroblasts

Abstract

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