Abstract
Objectives: We investigated the effect of transforming growth factor-β1 (TGF-β1) on the differentiation of nasal polyp-derived fibroblasts (NPDFs) and on their production of collagen, and investigated the effect of peroxisome proliferator-activated receptor γ (PPARγ) agonists on the effects of TGF-β1. Methods: Primary fibroblast lines were established from 5 human nasal polyp tissues. We activated the NPDFs with TGF-β1 and exposed them to the PPARγ agonists ciglitazone and 15-deoxy-Δ-prostaglandin J2. The expression of α-smooth muscle actin (α-SMA) and collagen was measured by reverse transcriptase-polymerase chain reactions, immunocytochemical staining, and collagen assays. Results: The treatment with TGF-β1 increased the expression of α-SMA and collagen, and expression was markedly attenuated by the PPARγ agonists. Conclusions: PPARγ agonists inhibit the differentiation of TGF-β1-activated NPDFs and their production of collagen.
| Original language | English |
|---|---|
| Pages (from-to) | 721-727 |
| Number of pages | 7 |
| Journal | Annals of Otology, Rhinology and Laryngology |
| Volume | 118 |
| Issue number | 10 |
| Publication status | Published - 2009 Oct 1 |
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Keywords
- Fibroblast
- Nasal polyp
- Peroxisome proliferator-activated receptor gamma agonist
ASJC Scopus subject areas
- Otorhinolaryngology
Cite this
Effect of peroxisome proliferator-activated receptor gamma agonists on myofibroblast differentiation and collagen production in nasal polyp-derived fibroblasts. / Lee, Heung Man; Kang, Hee Jun; Park, Hyo Hun; Hong, Seok Chan; Kim, Jin Kook; Cho, Jae Hoon.
In: Annals of Otology, Rhinology and Laryngology, Vol. 118, No. 10, 01.10.2009, p. 721-727.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of peroxisome proliferator-activated receptor gamma agonists on myofibroblast differentiation and collagen production in nasal polyp-derived fibroblasts
AU - Lee, Heung Man
AU - Kang, Hee Jun
AU - Park, Hyo Hun
AU - Hong, Seok Chan
AU - Kim, Jin Kook
AU - Cho, Jae Hoon
PY - 2009/10/1
Y1 - 2009/10/1
N2 - Objectives: We investigated the effect of transforming growth factor-β1 (TGF-β1) on the differentiation of nasal polyp-derived fibroblasts (NPDFs) and on their production of collagen, and investigated the effect of peroxisome proliferator-activated receptor γ (PPARγ) agonists on the effects of TGF-β1. Methods: Primary fibroblast lines were established from 5 human nasal polyp tissues. We activated the NPDFs with TGF-β1 and exposed them to the PPARγ agonists ciglitazone and 15-deoxy-Δ-prostaglandin J2. The expression of α-smooth muscle actin (α-SMA) and collagen was measured by reverse transcriptase-polymerase chain reactions, immunocytochemical staining, and collagen assays. Results: The treatment with TGF-β1 increased the expression of α-SMA and collagen, and expression was markedly attenuated by the PPARγ agonists. Conclusions: PPARγ agonists inhibit the differentiation of TGF-β1-activated NPDFs and their production of collagen.
AB - Objectives: We investigated the effect of transforming growth factor-β1 (TGF-β1) on the differentiation of nasal polyp-derived fibroblasts (NPDFs) and on their production of collagen, and investigated the effect of peroxisome proliferator-activated receptor γ (PPARγ) agonists on the effects of TGF-β1. Methods: Primary fibroblast lines were established from 5 human nasal polyp tissues. We activated the NPDFs with TGF-β1 and exposed them to the PPARγ agonists ciglitazone and 15-deoxy-Δ-prostaglandin J2. The expression of α-smooth muscle actin (α-SMA) and collagen was measured by reverse transcriptase-polymerase chain reactions, immunocytochemical staining, and collagen assays. Results: The treatment with TGF-β1 increased the expression of α-SMA and collagen, and expression was markedly attenuated by the PPARγ agonists. Conclusions: PPARγ agonists inhibit the differentiation of TGF-β1-activated NPDFs and their production of collagen.
KW - Fibroblast
KW - Nasal polyp
KW - Peroxisome proliferator-activated receptor gamma agonist
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M3 - Article
VL - 118
SP - 721
EP - 727
JO - Annals of Otology, Rhinology and Laryngology
JF - Annals of Otology, Rhinology and Laryngology
SN - 0003-4894
IS - 10
ER -