Effect of pH and oxygen on conjugated linoleic acid (CLA) production by mixed rumen bacteria from cows fed high concentrate and high forage diets

Nag Jin Choi, Jee Young Imm, Sejong Oh, Byoung-Chul Kim, Han Joon Hwang, Young Jun Kim

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Conjugated linoleic acid (CLA) production from linoleic acid (LA) was characterized, in a relationship to biohydrogenation (BH), with mixed rumen bacteria obtained from three cows fed a high concentrate diet (HCD; 630 g/kg) or a high forage diet (HFD; 770 g/kg) for 5 weeks. Mixed rumen bacteria were incubated with LA in a rumen fluid medium under various conditions. Rumen bacteria from cows fed HCD were more active (P < 0.05) in BH than those from cows fed the HFD at most tested pHs. Rumen bacteria from HFD fed cows produced mostly cis-9, trans-11 CLA at pHs higher than 6.2, but those from HCD fed cows produced more (P < 0.05) trans-10, cis-12 CLA than cis-9, trans-11 CLA at lower pHs. Production of cis-9, trans-11 CLA, positively correlated, and trans-10, cis-12 CLA inversely correlated, to pH with rumen bacteria from cows fed both diets (r2 = 0.88). Rumen bacteria from HCD fed cows accumulated about four times more cis-9, trans-11 CLA in aerobic conditions, versus anaerobic conditions, after 1 h of incubation. Overall, trans-10, cis-12 CLA producing rumen bacteria may be more acid-tolerant and aero-tolerant than cis-9, trans-11 CLA producing rumen bacteria.

Original languageEnglish
Pages (from-to)643-653
Number of pages11
JournalAnimal Feed Science and Technology
Volume123-124 PART 2
DOIs
Publication statusPublished - 2005 Dec 7

Fingerprint

Conjugated Linoleic Acids
rumen bacteria
Rumen
conjugated linoleic acid
concentrates
forage
Oxygen
Diet
Bacteria
oxygen
cows
diet
biohydrogenation
Linoleic Acid
linoleic acid
aerobic conditions
rumen fluids
anaerobic conditions
9,11-linoleic acid
Acids

Keywords

  • Aerobic condition
  • Conjugated linoleic acid
  • Diet
  • pH
  • Rumen bacteria

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Science
  • veterinary(all)

Cite this

Effect of pH and oxygen on conjugated linoleic acid (CLA) production by mixed rumen bacteria from cows fed high concentrate and high forage diets. / Choi, Nag Jin; Imm, Jee Young; Oh, Sejong; Kim, Byoung-Chul; Hwang, Han Joon; Kim, Young Jun.

In: Animal Feed Science and Technology, Vol. 123-124 PART 2, 07.12.2005, p. 643-653.

Research output: Contribution to journalArticle

@article{90adb7c2d46f48e5bbe5263f51bdfcf0,
title = "Effect of pH and oxygen on conjugated linoleic acid (CLA) production by mixed rumen bacteria from cows fed high concentrate and high forage diets",
abstract = "Conjugated linoleic acid (CLA) production from linoleic acid (LA) was characterized, in a relationship to biohydrogenation (BH), with mixed rumen bacteria obtained from three cows fed a high concentrate diet (HCD; 630 g/kg) or a high forage diet (HFD; 770 g/kg) for 5 weeks. Mixed rumen bacteria were incubated with LA in a rumen fluid medium under various conditions. Rumen bacteria from cows fed HCD were more active (P < 0.05) in BH than those from cows fed the HFD at most tested pHs. Rumen bacteria from HFD fed cows produced mostly cis-9, trans-11 CLA at pHs higher than 6.2, but those from HCD fed cows produced more (P < 0.05) trans-10, cis-12 CLA than cis-9, trans-11 CLA at lower pHs. Production of cis-9, trans-11 CLA, positively correlated, and trans-10, cis-12 CLA inversely correlated, to pH with rumen bacteria from cows fed both diets (r2 = 0.88). Rumen bacteria from HCD fed cows accumulated about four times more cis-9, trans-11 CLA in aerobic conditions, versus anaerobic conditions, after 1 h of incubation. Overall, trans-10, cis-12 CLA producing rumen bacteria may be more acid-tolerant and aero-tolerant than cis-9, trans-11 CLA producing rumen bacteria.",
keywords = "Aerobic condition, Conjugated linoleic acid, Diet, pH, Rumen bacteria",
author = "Choi, {Nag Jin} and Imm, {Jee Young} and Sejong Oh and Byoung-Chul Kim and Hwang, {Han Joon} and Kim, {Young Jun}",
year = "2005",
month = "12",
day = "7",
doi = "10.1016/j.anifeedsci.2005.04.054",
language = "English",
volume = "123-124 PART 2",
pages = "643--653",
journal = "Animal Feed Science and Technology",
issn = "0377-8401",
publisher = "Elsevier",

}

TY - JOUR

T1 - Effect of pH and oxygen on conjugated linoleic acid (CLA) production by mixed rumen bacteria from cows fed high concentrate and high forage diets

AU - Choi, Nag Jin

AU - Imm, Jee Young

AU - Oh, Sejong

AU - Kim, Byoung-Chul

AU - Hwang, Han Joon

AU - Kim, Young Jun

PY - 2005/12/7

Y1 - 2005/12/7

N2 - Conjugated linoleic acid (CLA) production from linoleic acid (LA) was characterized, in a relationship to biohydrogenation (BH), with mixed rumen bacteria obtained from three cows fed a high concentrate diet (HCD; 630 g/kg) or a high forage diet (HFD; 770 g/kg) for 5 weeks. Mixed rumen bacteria were incubated with LA in a rumen fluid medium under various conditions. Rumen bacteria from cows fed HCD were more active (P < 0.05) in BH than those from cows fed the HFD at most tested pHs. Rumen bacteria from HFD fed cows produced mostly cis-9, trans-11 CLA at pHs higher than 6.2, but those from HCD fed cows produced more (P < 0.05) trans-10, cis-12 CLA than cis-9, trans-11 CLA at lower pHs. Production of cis-9, trans-11 CLA, positively correlated, and trans-10, cis-12 CLA inversely correlated, to pH with rumen bacteria from cows fed both diets (r2 = 0.88). Rumen bacteria from HCD fed cows accumulated about four times more cis-9, trans-11 CLA in aerobic conditions, versus anaerobic conditions, after 1 h of incubation. Overall, trans-10, cis-12 CLA producing rumen bacteria may be more acid-tolerant and aero-tolerant than cis-9, trans-11 CLA producing rumen bacteria.

AB - Conjugated linoleic acid (CLA) production from linoleic acid (LA) was characterized, in a relationship to biohydrogenation (BH), with mixed rumen bacteria obtained from three cows fed a high concentrate diet (HCD; 630 g/kg) or a high forage diet (HFD; 770 g/kg) for 5 weeks. Mixed rumen bacteria were incubated with LA in a rumen fluid medium under various conditions. Rumen bacteria from cows fed HCD were more active (P < 0.05) in BH than those from cows fed the HFD at most tested pHs. Rumen bacteria from HFD fed cows produced mostly cis-9, trans-11 CLA at pHs higher than 6.2, but those from HCD fed cows produced more (P < 0.05) trans-10, cis-12 CLA than cis-9, trans-11 CLA at lower pHs. Production of cis-9, trans-11 CLA, positively correlated, and trans-10, cis-12 CLA inversely correlated, to pH with rumen bacteria from cows fed both diets (r2 = 0.88). Rumen bacteria from HCD fed cows accumulated about four times more cis-9, trans-11 CLA in aerobic conditions, versus anaerobic conditions, after 1 h of incubation. Overall, trans-10, cis-12 CLA producing rumen bacteria may be more acid-tolerant and aero-tolerant than cis-9, trans-11 CLA producing rumen bacteria.

KW - Aerobic condition

KW - Conjugated linoleic acid

KW - Diet

KW - pH

KW - Rumen bacteria

UR - http://www.scopus.com/inward/record.url?scp=28044437965&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28044437965&partnerID=8YFLogxK

U2 - 10.1016/j.anifeedsci.2005.04.054

DO - 10.1016/j.anifeedsci.2005.04.054

M3 - Article

AN - SCOPUS:28044437965

VL - 123-124 PART 2

SP - 643

EP - 653

JO - Animal Feed Science and Technology

JF - Animal Feed Science and Technology

SN - 0377-8401

ER -