Effect of pH and oxygen on conjugated linoleic acid (CLA) production by mixed rumen bacteria from cows fed high concentrate and high forage diets

Nag Jin Choi, Jee Young Imm, Sejong Oh, Byoung-Chul Kim, Han Joon Hwang, Young Jun Kim

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Conjugated linoleic acid (CLA) production from linoleic acid (LA) was characterized, in a relationship to biohydrogenation (BH), with mixed rumen bacteria obtained from three cows fed a high concentrate diet (HCD; 630 g/kg) or a high forage diet (HFD; 770 g/kg) for 5 weeks. Mixed rumen bacteria were incubated with LA in a rumen fluid medium under various conditions. Rumen bacteria from cows fed HCD were more active (P < 0.05) in BH than those from cows fed the HFD at most tested pHs. Rumen bacteria from HFD fed cows produced mostly cis-9, trans-11 CLA at pHs higher than 6.2, but those from HCD fed cows produced more (P < 0.05) trans-10, cis-12 CLA than cis-9, trans-11 CLA at lower pHs. Production of cis-9, trans-11 CLA, positively correlated, and trans-10, cis-12 CLA inversely correlated, to pH with rumen bacteria from cows fed both diets (r2 = 0.88). Rumen bacteria from HCD fed cows accumulated about four times more cis-9, trans-11 CLA in aerobic conditions, versus anaerobic conditions, after 1 h of incubation. Overall, trans-10, cis-12 CLA producing rumen bacteria may be more acid-tolerant and aero-tolerant than cis-9, trans-11 CLA producing rumen bacteria.

Original languageEnglish
Pages (from-to)643-653
Number of pages11
JournalAnimal Feed Science and Technology
Volume123-124 PART 2
DOIs
Publication statusPublished - 2005 Dec 7

Keywords

  • Aerobic condition
  • Conjugated linoleic acid
  • Diet
  • pH
  • Rumen bacteria

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Science
  • veterinary(all)

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