Effect of phospholipid insertion on arrayed polydiacetylene biosensors

Kyung Woo Kim, Hyun Choi, Gil Sun Lee, Dong June Ahn, Min Kyu Oh

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Micro-arrayed polydiacetylene (PDA) vesicles mixed with phospholipids on glass slides were prepared for label-free detection of Escherichia coli. When E. coli bound to its antibodies chemically attached to polydiacetylene, the fluorescence of the vesicles was dramatically increased. The insertion of dimyristoyl phosphatidylcholine (DMPC) in the vesicles drastically reduced the response time for the fluorescence changes. Vesicles with 20-30% DMPC provided optimal results for bacterial detection. Fourier transform infrared (FTIR) spectra analysis suggested that DMPC insertion decreased the strength of hydrogen bonding among the amide and carboxylic acid groups of the polydiacetylene vesicles. Reduced bonding strength resulted in less rigid structure of the polydiacetylene polymer, allowing more rapid detection upon molecular recognition.

Original languageEnglish
Pages (from-to)213-217
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume66
Issue number2
DOIs
Publication statusPublished - 2008 Oct 15

Keywords

  • Bacteria sensor
  • Dimyristoyl phosphatidylcholine (DMPC)
  • FTIR
  • Label-free biosensor
  • Polydiacetylene

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'Effect of phospholipid insertion on arrayed polydiacetylene biosensors'. Together they form a unique fingerprint.

  • Cite this