Effect of phospholipid insertion on arrayed polydiacetylene biosensors

Kyung W. Kim, Hyun Choi, Gil Sun Lee, Dong June Ahn, Min-Kyu Oh

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Micro-arrayed polydiacetylene (PDA) vesicles mixed with phospholipids on glass slides were prepared for label-free detection of Escherichia coli. When E. coli bound to its antibodies chemically attached to polydiacetylene, the fluorescence of the vesicles was dramatically increased. The insertion of dimyristoyl phosphatidylcholine (DMPC) in the vesicles drastically reduced the response time for the fluorescence changes. Vesicles with 20-30% DMPC provided optimal results for bacterial detection. Fourier transform infrared (FTIR) spectra analysis suggested that DMPC insertion decreased the strength of hydrogen bonding among the amide and carboxylic acid groups of the polydiacetylene vesicles. Reduced bonding strength resulted in less rigid structure of the polydiacetylene polymer, allowing more rapid detection upon molecular recognition.

Original languageEnglish
Pages (from-to)213-217
Number of pages5
JournalColloids and Surfaces B: Biointerfaces
Volume66
Issue number2
DOIs
Publication statusPublished - 2008 Oct 15

Fingerprint

Phospholipids
Biosensing Techniques
bioinstrumentation
Biosensors
Escherichia coli
insertion
Fluorescence
Phosphatidylcholines
Rigid structures
Molecular recognition
Carboxylic acids
Amides
Antibodies
Spectrum analysis
Labels
fluorescence
Fourier transforms
Hydrogen bonds
rigid structures
Escherichia

Keywords

  • Bacteria sensor
  • Dimyristoyl phosphatidylcholine (DMPC)
  • FTIR
  • Label-free biosensor
  • Polydiacetylene

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Effect of phospholipid insertion on arrayed polydiacetylene biosensors. / Kim, Kyung W.; Choi, Hyun; Lee, Gil Sun; Ahn, Dong June; Oh, Min-Kyu.

In: Colloids and Surfaces B: Biointerfaces, Vol. 66, No. 2, 15.10.2008, p. 213-217.

Research output: Contribution to journalArticle

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