Abstract
Micro-arrayed polydiacetylene (PDA) vesicles mixed with phospholipids on glass slides were prepared for label-free detection of Escherichia coli. When E. coli bound to its antibodies chemically attached to polydiacetylene, the fluorescence of the vesicles was dramatically increased. The insertion of dimyristoyl phosphatidylcholine (DMPC) in the vesicles drastically reduced the response time for the fluorescence changes. Vesicles with 20-30% DMPC provided optimal results for bacterial detection. Fourier transform infrared (FTIR) spectra analysis suggested that DMPC insertion decreased the strength of hydrogen bonding among the amide and carboxylic acid groups of the polydiacetylene vesicles. Reduced bonding strength resulted in less rigid structure of the polydiacetylene polymer, allowing more rapid detection upon molecular recognition.
| Original language | English |
|---|---|
| Pages (from-to) | 213-217 |
| Number of pages | 5 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 66 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2008 Oct 15 |
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Keywords
- Bacteria sensor
- Dimyristoyl phosphatidylcholine (DMPC)
- FTIR
- Label-free biosensor
- Polydiacetylene
ASJC Scopus subject areas
- Biotechnology
- Colloid and Surface Chemistry
- Physical and Theoretical Chemistry
- Surfaces and Interfaces
Cite this
Effect of phospholipid insertion on arrayed polydiacetylene biosensors. / Kim, Kyung W.; Choi, Hyun; Lee, Gil Sun; Ahn, Dong June; Oh, Min-Kyu.
In: Colloids and Surfaces B: Biointerfaces, Vol. 66, No. 2, 15.10.2008, p. 213-217.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of phospholipid insertion on arrayed polydiacetylene biosensors
AU - Kim, Kyung W.
AU - Choi, Hyun
AU - Lee, Gil Sun
AU - Ahn, Dong June
AU - Oh, Min-Kyu
PY - 2008/10/15
Y1 - 2008/10/15
N2 - Micro-arrayed polydiacetylene (PDA) vesicles mixed with phospholipids on glass slides were prepared for label-free detection of Escherichia coli. When E. coli bound to its antibodies chemically attached to polydiacetylene, the fluorescence of the vesicles was dramatically increased. The insertion of dimyristoyl phosphatidylcholine (DMPC) in the vesicles drastically reduced the response time for the fluorescence changes. Vesicles with 20-30% DMPC provided optimal results for bacterial detection. Fourier transform infrared (FTIR) spectra analysis suggested that DMPC insertion decreased the strength of hydrogen bonding among the amide and carboxylic acid groups of the polydiacetylene vesicles. Reduced bonding strength resulted in less rigid structure of the polydiacetylene polymer, allowing more rapid detection upon molecular recognition.
AB - Micro-arrayed polydiacetylene (PDA) vesicles mixed with phospholipids on glass slides were prepared for label-free detection of Escherichia coli. When E. coli bound to its antibodies chemically attached to polydiacetylene, the fluorescence of the vesicles was dramatically increased. The insertion of dimyristoyl phosphatidylcholine (DMPC) in the vesicles drastically reduced the response time for the fluorescence changes. Vesicles with 20-30% DMPC provided optimal results for bacterial detection. Fourier transform infrared (FTIR) spectra analysis suggested that DMPC insertion decreased the strength of hydrogen bonding among the amide and carboxylic acid groups of the polydiacetylene vesicles. Reduced bonding strength resulted in less rigid structure of the polydiacetylene polymer, allowing more rapid detection upon molecular recognition.
KW - Bacteria sensor
KW - Dimyristoyl phosphatidylcholine (DMPC)
KW - FTIR
KW - Label-free biosensor
KW - Polydiacetylene
UR - http://www.scopus.com/inward/record.url?scp=50649088906&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50649088906&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2008.06.020
DO - 10.1016/j.colsurfb.2008.06.020
M3 - Article
C2 - 18701264
AN - SCOPUS:50649088906
VL - 66
SP - 213
EP - 217
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
IS - 2
ER -