Effect of phospholipid insertion on arrayed polydiacetylene biosensors

Kyung Woo Kim; Hyun Choi; Gil Sun Lee; Dong June Ahn; Min Kyu Oh

doi:10.1016/j.colsurfb.2008.06.020

Effect of phospholipid insertion on arrayed polydiacetylene biosensors

Kyung Woo Kim, Hyun Choi, Gil Sun Lee, Dong June Ahn, Min Kyu Oh

Research output: Contribution to journal › Article

40 Citations (Scopus)

Abstract

Micro-arrayed polydiacetylene (PDA) vesicles mixed with phospholipids on glass slides were prepared for label-free detection of Escherichia coli. When E. coli bound to its antibodies chemically attached to polydiacetylene, the fluorescence of the vesicles was dramatically increased. The insertion of dimyristoyl phosphatidylcholine (DMPC) in the vesicles drastically reduced the response time for the fluorescence changes. Vesicles with 20-30% DMPC provided optimal results for bacterial detection. Fourier transform infrared (FTIR) spectra analysis suggested that DMPC insertion decreased the strength of hydrogen bonding among the amide and carboxylic acid groups of the polydiacetylene vesicles. Reduced bonding strength resulted in less rigid structure of the polydiacetylene polymer, allowing more rapid detection upon molecular recognition.

Original language	English
Pages (from-to)	213-217
Number of pages	5
Journal	Colloids and Surfaces B: Biointerfaces
Volume	66
Issue number	2
DOIs	https://doi.org/10.1016/j.colsurfb.2008.06.020
Publication status	Published - 2008 Oct 15

Keywords

Bacteria sensor
Dimyristoyl phosphatidylcholine (DMPC)
FTIR
Label-free biosensor
Polydiacetylene

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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Kim, K. W., Choi, H., Lee, G. S., Ahn, D. J., & Oh, M. K. (2008). Effect of phospholipid insertion on arrayed polydiacetylene biosensors. Colloids and Surfaces B: Biointerfaces, 66(2), 213-217. https://doi.org/10.1016/j.colsurfb.2008.06.020

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abstract = "Micro-arrayed polydiacetylene (PDA) vesicles mixed with phospholipids on glass slides were prepared for label-free detection of Escherichia coli. When E. coli bound to its antibodies chemically attached to polydiacetylene, the fluorescence of the vesicles was dramatically increased. The insertion of dimyristoyl phosphatidylcholine (DMPC) in the vesicles drastically reduced the response time for the fluorescence changes. Vesicles with 20-30% DMPC provided optimal results for bacterial detection. Fourier transform infrared (FTIR) spectra analysis suggested that DMPC insertion decreased the strength of hydrogen bonding among the amide and carboxylic acid groups of the polydiacetylene vesicles. Reduced bonding strength resulted in less rigid structure of the polydiacetylene polymer, allowing more rapid detection upon molecular recognition.",

keywords = "Bacteria sensor, Dimyristoyl phosphatidylcholine (DMPC), FTIR, Label-free biosensor, Polydiacetylene",

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AU - Oh, Min Kyu

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