Effect of poly(ethylene glycol) graft polymerization of poly(methyl methacrylate) on cell adhesion: In vitro and in vivo study

Mee Kum Kim, In Suk Park, Hyung Dal Park, Won Ryang Wee, Jin Hak Lee, Ki Dong Park, Soo Hyun Kim, Young Ha Kim

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Purpose: To investigate the effect of surface modification of poly(methyl methacrylate) (PMMA) by poly(ethylene glycol) (PEG) grafting on cell adhesion. Setting: Department of Ophthalmology, Seoul National University Hospital, Seoul, Korea. Methods: The PMMA surface was oxidized with ozone, and PEG acrylate was then graft polymerized. To verify the PEG grafting on the surface, the oxygen content was measured by electron spectroscopy for chemical analysis. The contact angle was measured using the Wilhelmy plate method. The adhesion of keratocytes on modified PMMA was investigated in vitro. Cultured rabbit keratocytes (4 × 105 cells/mL) were layered on each PMMA disk, cultured in a carbon dioxide incubator for 24 hours, harvested by trypsinization, and counted. A commercially available intraocular lens was modified as described and then inserted in the anterior chamber of a white rabbit. The cell adherence pattern on the modified IOL was examined by scanning electron microscopy. Results: The PEG-grafted PMMA revealed a higher oxygen content and lower dynamic receding contact angles than the untreated PMMA. The mean number of adhered cells was 72.5 ± 22 × 104/mL for untreated PMMA. After PEG grafting of 1 hour and ozone oxidation of 2 hours, the adherent cell counts significantly decreased to 6.5 ± 1.7 × 104/mL and 7.6 ± 1.6 × 104/mL, respectively (P = .002). Scanning electron microscopy showed small round cells sparsely scattered on the modified PMMA in contrast to the untreated PMMA. Conclusion: Surface modification of PMMA using PEG grafting reduced cell adhesion. This may decrease the incidence of retroprosthetic membrane formation after keratoprosthesis surgery.

Original languageEnglish
Pages (from-to)766-774
Number of pages9
JournalJournal of Cataract and Refractive Surgery
Volume27
Issue number5
DOIs
Publication statusPublished - 2001 May 1
Externally publishedYes

Fingerprint

Ethylene Glycol
Polymethyl Methacrylate
Cell Adhesion
Polymerization
Transplants
Ozone
In Vitro Techniques
Electron Scanning Microscopy
Cell Count
Oxygen
Rabbits
Incubators
Photoelectron Spectroscopy
Intraocular Lenses
Anterior Chamber
Ophthalmology
Korea
Carbon Dioxide

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Effect of poly(ethylene glycol) graft polymerization of poly(methyl methacrylate) on cell adhesion : In vitro and in vivo study. / Kim, Mee Kum; Park, In Suk; Park, Hyung Dal; Wee, Won Ryang; Lee, Jin Hak; Park, Ki Dong; Kim, Soo Hyun; Kim, Young Ha.

In: Journal of Cataract and Refractive Surgery, Vol. 27, No. 5, 01.05.2001, p. 766-774.

Research output: Contribution to journalArticle

Kim, Mee Kum ; Park, In Suk ; Park, Hyung Dal ; Wee, Won Ryang ; Lee, Jin Hak ; Park, Ki Dong ; Kim, Soo Hyun ; Kim, Young Ha. / Effect of poly(ethylene glycol) graft polymerization of poly(methyl methacrylate) on cell adhesion : In vitro and in vivo study. In: Journal of Cataract and Refractive Surgery. 2001 ; Vol. 27, No. 5. pp. 766-774.
@article{b70ca46a31da4c9680654858af945ab8,
title = "Effect of poly(ethylene glycol) graft polymerization of poly(methyl methacrylate) on cell adhesion: In vitro and in vivo study",
abstract = "Purpose: To investigate the effect of surface modification of poly(methyl methacrylate) (PMMA) by poly(ethylene glycol) (PEG) grafting on cell adhesion. Setting: Department of Ophthalmology, Seoul National University Hospital, Seoul, Korea. Methods: The PMMA surface was oxidized with ozone, and PEG acrylate was then graft polymerized. To verify the PEG grafting on the surface, the oxygen content was measured by electron spectroscopy for chemical analysis. The contact angle was measured using the Wilhelmy plate method. The adhesion of keratocytes on modified PMMA was investigated in vitro. Cultured rabbit keratocytes (4 × 105 cells/mL) were layered on each PMMA disk, cultured in a carbon dioxide incubator for 24 hours, harvested by trypsinization, and counted. A commercially available intraocular lens was modified as described and then inserted in the anterior chamber of a white rabbit. The cell adherence pattern on the modified IOL was examined by scanning electron microscopy. Results: The PEG-grafted PMMA revealed a higher oxygen content and lower dynamic receding contact angles than the untreated PMMA. The mean number of adhered cells was 72.5 ± 22 × 104/mL for untreated PMMA. After PEG grafting of 1 hour and ozone oxidation of 2 hours, the adherent cell counts significantly decreased to 6.5 ± 1.7 × 104/mL and 7.6 ± 1.6 × 104/mL, respectively (P = .002). Scanning electron microscopy showed small round cells sparsely scattered on the modified PMMA in contrast to the untreated PMMA. Conclusion: Surface modification of PMMA using PEG grafting reduced cell adhesion. This may decrease the incidence of retroprosthetic membrane formation after keratoprosthesis surgery.",
author = "Kim, {Mee Kum} and Park, {In Suk} and Park, {Hyung Dal} and Wee, {Won Ryang} and Lee, {Jin Hak} and Park, {Ki Dong} and Kim, {Soo Hyun} and Kim, {Young Ha}",
year = "2001",
month = "5",
day = "1",
doi = "10.1016/S0886-3350(00)00701-X",
language = "English",
volume = "27",
pages = "766--774",
journal = "Journal of Cataract and Refractive Surgery",
issn = "0886-3350",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Effect of poly(ethylene glycol) graft polymerization of poly(methyl methacrylate) on cell adhesion

T2 - In vitro and in vivo study

AU - Kim, Mee Kum

AU - Park, In Suk

AU - Park, Hyung Dal

AU - Wee, Won Ryang

AU - Lee, Jin Hak

AU - Park, Ki Dong

AU - Kim, Soo Hyun

AU - Kim, Young Ha

PY - 2001/5/1

Y1 - 2001/5/1

N2 - Purpose: To investigate the effect of surface modification of poly(methyl methacrylate) (PMMA) by poly(ethylene glycol) (PEG) grafting on cell adhesion. Setting: Department of Ophthalmology, Seoul National University Hospital, Seoul, Korea. Methods: The PMMA surface was oxidized with ozone, and PEG acrylate was then graft polymerized. To verify the PEG grafting on the surface, the oxygen content was measured by electron spectroscopy for chemical analysis. The contact angle was measured using the Wilhelmy plate method. The adhesion of keratocytes on modified PMMA was investigated in vitro. Cultured rabbit keratocytes (4 × 105 cells/mL) were layered on each PMMA disk, cultured in a carbon dioxide incubator for 24 hours, harvested by trypsinization, and counted. A commercially available intraocular lens was modified as described and then inserted in the anterior chamber of a white rabbit. The cell adherence pattern on the modified IOL was examined by scanning electron microscopy. Results: The PEG-grafted PMMA revealed a higher oxygen content and lower dynamic receding contact angles than the untreated PMMA. The mean number of adhered cells was 72.5 ± 22 × 104/mL for untreated PMMA. After PEG grafting of 1 hour and ozone oxidation of 2 hours, the adherent cell counts significantly decreased to 6.5 ± 1.7 × 104/mL and 7.6 ± 1.6 × 104/mL, respectively (P = .002). Scanning electron microscopy showed small round cells sparsely scattered on the modified PMMA in contrast to the untreated PMMA. Conclusion: Surface modification of PMMA using PEG grafting reduced cell adhesion. This may decrease the incidence of retroprosthetic membrane formation after keratoprosthesis surgery.

AB - Purpose: To investigate the effect of surface modification of poly(methyl methacrylate) (PMMA) by poly(ethylene glycol) (PEG) grafting on cell adhesion. Setting: Department of Ophthalmology, Seoul National University Hospital, Seoul, Korea. Methods: The PMMA surface was oxidized with ozone, and PEG acrylate was then graft polymerized. To verify the PEG grafting on the surface, the oxygen content was measured by electron spectroscopy for chemical analysis. The contact angle was measured using the Wilhelmy plate method. The adhesion of keratocytes on modified PMMA was investigated in vitro. Cultured rabbit keratocytes (4 × 105 cells/mL) were layered on each PMMA disk, cultured in a carbon dioxide incubator for 24 hours, harvested by trypsinization, and counted. A commercially available intraocular lens was modified as described and then inserted in the anterior chamber of a white rabbit. The cell adherence pattern on the modified IOL was examined by scanning electron microscopy. Results: The PEG-grafted PMMA revealed a higher oxygen content and lower dynamic receding contact angles than the untreated PMMA. The mean number of adhered cells was 72.5 ± 22 × 104/mL for untreated PMMA. After PEG grafting of 1 hour and ozone oxidation of 2 hours, the adherent cell counts significantly decreased to 6.5 ± 1.7 × 104/mL and 7.6 ± 1.6 × 104/mL, respectively (P = .002). Scanning electron microscopy showed small round cells sparsely scattered on the modified PMMA in contrast to the untreated PMMA. Conclusion: Surface modification of PMMA using PEG grafting reduced cell adhesion. This may decrease the incidence of retroprosthetic membrane formation after keratoprosthesis surgery.

UR - http://www.scopus.com/inward/record.url?scp=0034996274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034996274&partnerID=8YFLogxK

U2 - 10.1016/S0886-3350(00)00701-X

DO - 10.1016/S0886-3350(00)00701-X

M3 - Article

C2 - 11377910

AN - SCOPUS:0034996274

VL - 27

SP - 766

EP - 774

JO - Journal of Cataract and Refractive Surgery

JF - Journal of Cataract and Refractive Surgery

SN - 0886-3350

IS - 5

ER -