Effect of porcine placenta extract from subcritical water extraction on photodamage in human keratinocytes

Yooheon Park, Bok Kyung Han, Hyeon Son Choi, Yang Hee Hong, Eun Young Jung, Hyung Joo Suh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The objective of this study was to evaluated the photoprotective effects of porcine placenta extract (PPE) on ultraviolet B (UVB)-induced oxidative stress in human keratinocytes (HaCaT) to evaluate its functional activities as a skin food ingredient. PPE prepared by subcritical water extraction was termed SPE, and subsequently digested by enzymes to prepare E-SPE. Increased intracellular reactive oxygen species (ROS) levels (192.0%) induced by UVB were decreased by SPE and E-SPE. SPE had more effective ROS scavenging activity than E-SPE treatment. UVB treatment increased expression of tissue inhibitor of metalloproteinase 1 (TIMP-1), and this elevated expression was decreased by E-SPE treatment. High-dose treatment with E-SPE (50 and 100 μg/mL) reduced TIMP-1 expression levels of UVB-C (control) to 33.5 and 34.6%, respectively. In contrast, at low SPE doses (1 and 10 μg/mL), the treatment slightly decreased TIMP-1 expression levels to 73.3% and 71.3% of UVB-C, respectively. In conclusion, the present study demonstrated the protective effect of SPE and E-SPE against UVB damage in keratinocytes via ROS scavenging, down-regulating MMP-2 expression and up-regulating TIMP-1 expression. This highlights the potential for SPE as an ingredient in the preparation of functional food against photoaging.

Original languageEnglish
Pages (from-to)164-170
Number of pages7
JournalKorean Journal for Food Science of Animal Resources
Volume35
Issue number2
DOIs
Publication statusPublished - 2015 Apr 1

Fingerprint

Tissue Inhibitor of Metalloproteinase-1
keratinocytes
placenta
Keratinocytes
Placenta
ultraviolet radiation
Swine
metalloproteinases
Reactive Oxygen Species
swine
Water
extracts
reactive oxygen species
water
Functional Food
ingredients
photoaging
Matrix Metalloproteinases
gelatinase A
Oxidative Stress

Keywords

  • MMP-2
  • Porcine placenta extract
  • ROS
  • Subcritical water extraction
  • TIMP-1
  • UVB

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology

Cite this

Effect of porcine placenta extract from subcritical water extraction on photodamage in human keratinocytes. / Park, Yooheon; Han, Bok Kyung; Choi, Hyeon Son; Hong, Yang Hee; Jung, Eun Young; Suh, Hyung Joo.

In: Korean Journal for Food Science of Animal Resources, Vol. 35, No. 2, 01.04.2015, p. 164-170.

Research output: Contribution to journalArticle

Park, Yooheon ; Han, Bok Kyung ; Choi, Hyeon Son ; Hong, Yang Hee ; Jung, Eun Young ; Suh, Hyung Joo. / Effect of porcine placenta extract from subcritical water extraction on photodamage in human keratinocytes. In: Korean Journal for Food Science of Animal Resources. 2015 ; Vol. 35, No. 2. pp. 164-170.
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