Effect of pregnancy and progesterone concentration on expression of genes encoding for transporters or secreted proteins in the bovine endometrium

N. Forde, T. E. Spencer, F. W. Bazer, Gwonhwa Song, J. F. Roche, P. Lonergan

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The objective of this study was to determine the temporal and spatial expression patterns of genes encoding transporters, as well as selected secreted proteins that may be regulated by progesterone (P4) and/or the presence of the conceptus in the bovine endometrium. Estrus-synchronized beef heifers were randomly assigned to either: 1) pregnant, high P4; 2) pregnant, normal P4; 3) cyclic, high P4; or 4) cyclic, normal P4. Uteri were collected on days 5, 7, 13, and 16 of the estrous cycle or pregnancy. Localization of mRNAs for ANPEP, CTGF, LPL, LTF, and SLC5A1 in the uteri was determined by radioactive in situ hybridization, and expression quantified in the endometria by quantitative real-time PCR. ANPEP localized to luminal (LE) and super-ficial glandular (sGE) epithelia of all heifers on days 5 and 7 only. SLC5A1 mRNA was detected in the LE and sGE on days 13 and 16 in all heifers, and expression increased on day 16 in pregnant groups. CTGF localized weakly to the LE and GE on days 5 and 7 but increased on days 13 and 16 with an increase (P < 0.05) in CTGF expression in high P4 (day 7) and pregnant heifers (day 16). Both LPL and LTF localized to the GE only on days 5 and 7. In conclusion we have characterized the temporal expression pattern of these genes and modulation of their transcript abundance by P4 (CTGF, LPL) and/or the conceptus (CTGF, SLC5A1) likely modifies the uterine microenvironment, enhancing histotroph composition and contributing to advanced conceptus elongation.

Original languageEnglish
Pages (from-to)53-62
Number of pages10
JournalPhysiological Genomics
Volume41
Issue number1
DOIs
Publication statusPublished - 2010 Mar 1
Externally publishedYes

Fingerprint

Endometrium
Uterus
Progesterone
Gene Expression
Pregnancy
Messenger RNA
Estrous Cycle
Estrus
In Situ Hybridization
Real-Time Polymerase Chain Reaction
Proteins
Epithelium

Keywords

  • Gene expression
  • Gene localization

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Effect of pregnancy and progesterone concentration on expression of genes encoding for transporters or secreted proteins in the bovine endometrium. / Forde, N.; Spencer, T. E.; Bazer, F. W.; Song, Gwonhwa; Roche, J. F.; Lonergan, P.

In: Physiological Genomics, Vol. 41, No. 1, 01.03.2010, p. 53-62.

Research output: Contribution to journalArticle

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