Effect of pressure on catalytic properties of glutamate racemase from Aquifex pyrophilus, an extremophilic bacteria

A. Lee, Ki Seog, Young Min Chi, Yeon Gyu Yu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of pressure on the catalytic properties of glutamate racemase from Aquifex pyrophilus, an extremophilic bacterium, was investigated. The activation volume for the overall reaction (ΔV) and catalysis (ΔVcat) was -96.97 ml/mol and 4.97 ml/mol, respectively, while the reaction volume for the substrate binding (ΔVKm-1) was -101.94 ml/mol. The large negative ΔV for the overall reaction indicated that the pressurization of glutamate racemase resulted in enhanced catalytic efficiencies. In addition, this value was also due to the large negative ΔVKm-1 for the substrate binding. The negative value of ΔVKm-1 implied that the conformational changes in the enzyme molecule occurred during the substrate binding process, thereby increasing the degree of hydration. The small value of ΔVcat suggested that the pressure did not affect the glutamate racemase catalysis after the substrate binding.

Original languageEnglish
Pages (from-to)149-152
Number of pages4
JournalJournal of microbiology and biotechnology
Volume12
Issue number1
Publication statusPublished - 2002 Jan 1

    Fingerprint

Keywords

  • Activation volume
  • Aquifex pyrophilus
  • Extremophiles
  • Glutamate racemase
  • High pressure

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this