Effect of propofol on cardiac function and gene expression after ischemic-reperfusion in isolated rat heart

Jin Kim Youn, Hae Ja Lim, Sung Uk Choi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: The aim of this study was to examine the cardiac function and transcriptional response of the heart to propofol after ischemia-reperfusion. Methods: Rat hearts were Langendorff-perfused using the modified Krebs-Henseleit buffer, and took 20 min stabilizing periods, 40 min ischemia periods, and then 120 min reperfusion period. The hearts were divided into 5 groups; Control: 180 min perfusion after stabilization, Ischemic: 40 min global ischemia after stabilization, followed by 120 min reperfusion, Pre: 2 μM propofol treatment was preformed only before ischemia, Post: 2 μM propofol treatment was performed only during reperfusion after ischemia, Pre/Post: 2 μM propofol treatment was performed both before and after ischemia. The measurement for cardiac performances, such as left ventricular developed pressure (LVDP), rate of left ventricular pressure generation (dP/dt), heart rate, and coronary flow were obtained. The expression profiles of isolated mRNA were determined by using Agilent microarray and real time-polymerase chain reaction (RT-PCR) was used to confirm the microarray results for a subset of genes. Results: The Post group showed better LVDP and dP/dt than the Ischemic group. But there were no significant differences in heart rate and coronary flow among the groups. On the results of RT-PCR, the expressions of Abcc9, Bard1, and Casp4 were increased, but the expressions of Lyz, Casp8, and Timp1 were decreased in the Post group compared with the Ischemic group. Conclusions: This study suggests that 2 μM propofol may provide cardioprotective effect, and modulate gene expression such as apoptosis, and KATP ion channel related-genes during reperfusion in the isolated rat hearts.

Original languageEnglish
Pages (from-to)153-161
Number of pages9
JournalKorean Journal of Anesthesiology
Volume58
Issue number2
DOIs
Publication statusPublished - 2010 Feb 1

Keywords

  • Ischemia
  • Microarray
  • Propofol
  • Rat heart
  • Real time-polymerase chain reaction
  • Reperfusion

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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