Effect of quercetin-rich onion peel extracts on arterial thrombosis in rats

Seung Min Lee, Jiyoung Moon, Ji Hyung Chung, Yong Jun Cha, Min-Jeong Shin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The aim of this study was to examine whether oral supplementation of quercetin-rich onion peel extract (OPE) influences blood coagulation and arterial thrombosis in Sprague-Dawley (SD) rats. 24 male rats, 5. weeks old, were divided into three groups with different diets (C: control, 2. mg OPE: chow diet with 2. mg OPE supplementation, 10. mg OPE: chow diet with 10. mg OPE supplementation) for 6. weeks. Blood coagulation parameters including prothrombin time (PT), activated partial thromboplastin time (aPTT) and platelet aggregation were examined. The OPE did not affect blood cholesterol levels but significantly decreased blood triglyceride and glucose levels. PT, aPTT and platelet aggregation were not significantly different among all tested groups. However, in vivo arterial thrombosis was significantly delayed in groups that were fed 2. mg and 10. mg OPE diets compared to the control group. In addition, the OPE greatly diminished thrombin-induced expression of tissue factor in human umbilical vein endothelial cells (HUVECs), a coagulation initiator. In addition, extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) signaling pathways activated by thrombin treatment were prevented by the OPE pre-treatment. These results indicate that OPE may have anti-thrombotic effects through restricting the induced expression of tissue factor via down-regulating mitogen-activated protein kinase (MAPK) activation upon coagulation stimulus, leading to the prolongation of time for arterial thrombosis.

Original languageEnglish
Pages (from-to)99-105
Number of pages7
JournalFood and Chemical Toxicology
Volume57
DOIs
Publication statusPublished - 2013 Jul 1

Fingerprint

Onions
thrombosis
Quercetin
Thromboplastin
Nutrition
Coagulation
onions
quercetin
Rats
Thrombosis
Blood
rats
extracts
Prothrombin
Platelets
Thrombin
Agglomeration
mitogen-activated protein kinase
JNK Mitogen-Activated Protein Kinases
Extracellular Signal-Regulated MAP Kinases

Keywords

  • APTT
  • Arterial thrombosis
  • Blood coagulation
  • Onion peel extract
  • Quercetin

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Effect of quercetin-rich onion peel extracts on arterial thrombosis in rats. / Lee, Seung Min; Moon, Jiyoung; Chung, Ji Hyung; Cha, Yong Jun; Shin, Min-Jeong.

In: Food and Chemical Toxicology, Vol. 57, 01.07.2013, p. 99-105.

Research output: Contribution to journalArticle

Lee, Seung Min ; Moon, Jiyoung ; Chung, Ji Hyung ; Cha, Yong Jun ; Shin, Min-Jeong. / Effect of quercetin-rich onion peel extracts on arterial thrombosis in rats. In: Food and Chemical Toxicology. 2013 ; Vol. 57. pp. 99-105.
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