Effect of radiation scattering on dose uniformity in open and closed cell culture vessels

Myonggeun Yoon, Sung Yong Park, Jungwook Shin, Jungwon Kwak, Junghun Park, Dongho Shin, Soah Park, Se Byeong Lee, Doo Hyun Lee, Kyung Hwan Shin, Hong Ryull Pyo, Joo Young Kim, Kwan Ho Cho

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: Dose uniformity in cell culture vessels such as Petri dishes and anoxic irradiation chambers is very important in radiobiological work as dose uniformity affects cell survival probabilities. In this study, we investigated X-ray dose inhomogeneity, caused by scattering, in typical culture vessels. Materials and methods: Three different cubic cell culture vessels, with side lengths of 10 cm, 15 cm and 20 cm, were designed and irradiated by X-rays of 6 MV and 15 MV at a source-to-surface distance (SSD) of 100 cm using a Varian 2100CD linear accelerator. Results: The relative X-ray dose distribution in a cell culture vessel depended strongly on whether the vessel had a lid. The percentage of the cell culture surface with the dose differing by more than 10% from the mean value of the dose was 43.4% in lidless vessels and 9.7% in lidded vessels. Conclusions: In radiobiological work, X-ray dose inhomogeneity within a cell culture vessel is not negligible and the placement of cells in the vessel should be carefully considered.

Original languageEnglish
Pages (from-to)561-566
Number of pages6
JournalInternational Journal of Radiation Biology
Volume83
Issue number8
DOIs
Publication statusPublished - 2007
Externally publishedYes

Keywords

  • Cell culture vessel
  • Homogeneity
  • Oxygen enhancement ratio
  • Radiobiology
  • Scattering

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Yoon, M., Park, S. Y., Shin, J., Kwak, J., Park, J., Shin, D., Park, S., Lee, S. B., Lee, D. H., Shin, K. H., Pyo, H. R., Kim, J. Y., & Cho, K. H. (2007). Effect of radiation scattering on dose uniformity in open and closed cell culture vessels. International Journal of Radiation Biology, 83(8), 561-566. https://doi.org/10.1080/09553000701383178