Abstract
Background and Aims: Recent reports suggest that platelet-activating factor (PAF) plays a role in pancreatitis and pancreatitis-associated lung injury. In this study, the effects on these processes of termination of PAF action by recombinant PAF-acetylhydrolase (rPAF-AH) were investigated. Methods: Rats were given rPAF-AH and then infused with a supramaximally stimulating dose of cerulein to induce mild pancreatitis. Opossums underwent biliopancreatic duct ligation to induce severe pancreatitis, and rPAF-AH administration was begun 2 days later. Results: In mild, secretagogue- induced pancreatitis, rPAF-AH given before the cerulein reduced hyperamylasemia, acinar cell vacuolization, and pancreatic inflammation but did not alter pancreatic edema or pulmonary microvascular permeability. In severe, biliopancreatic duct ligation-induced pancreatitis, rPAF-AH delayed and reduced the extent of inflammation and acinar cell injury/necrosis and completely prevented lung injury even though the rPAF-AH administration was begun after the onset of pancreatitis. Conclusions: PAF plays an important role in the regulation of pancreatic injury but not pancreatic edema or increased pulmonary microvascular permeability in mild, secretagogue-induced pancreatitis. PAF plays a critical role in the regulation of progression of pancreatic injury and mediation of pancreatitis-associated lung injury in severe biliary pancreatitis. Amelioration of pancreatitis and prevention of pancreatitis-associated lung injury can be achieved with rPAF-AH even if treatment is begun after pancreatitis is established.
| Original language | English |
|---|---|
| Pages (from-to) | 1238-1247 |
| Number of pages | 10 |
| Journal | Gastroenterology |
| Volume | 115 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1998 Jan 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Gastroenterology
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Effect of recombinant platelet-activating factor acetylhydrolase on two models of experimental acute pancreatitis. / Hofbauer, B.; Saluja, A. K.; Bhatia, M.; Frossard, J. L.; Lee, Hong Sik; Bhagat, L.; Steer, M. L.
In: Gastroenterology, Vol. 115, No. 5, 01.01.1998, p. 1238-1247.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of recombinant platelet-activating factor acetylhydrolase on two models of experimental acute pancreatitis
AU - Hofbauer, B.
AU - Saluja, A. K.
AU - Bhatia, M.
AU - Frossard, J. L.
AU - Lee, Hong Sik
AU - Bhagat, L.
AU - Steer, M. L.
PY - 1998/1/1
Y1 - 1998/1/1
N2 - Background and Aims: Recent reports suggest that platelet-activating factor (PAF) plays a role in pancreatitis and pancreatitis-associated lung injury. In this study, the effects on these processes of termination of PAF action by recombinant PAF-acetylhydrolase (rPAF-AH) were investigated. Methods: Rats were given rPAF-AH and then infused with a supramaximally stimulating dose of cerulein to induce mild pancreatitis. Opossums underwent biliopancreatic duct ligation to induce severe pancreatitis, and rPAF-AH administration was begun 2 days later. Results: In mild, secretagogue- induced pancreatitis, rPAF-AH given before the cerulein reduced hyperamylasemia, acinar cell vacuolization, and pancreatic inflammation but did not alter pancreatic edema or pulmonary microvascular permeability. In severe, biliopancreatic duct ligation-induced pancreatitis, rPAF-AH delayed and reduced the extent of inflammation and acinar cell injury/necrosis and completely prevented lung injury even though the rPAF-AH administration was begun after the onset of pancreatitis. Conclusions: PAF plays an important role in the regulation of pancreatic injury but not pancreatic edema or increased pulmonary microvascular permeability in mild, secretagogue-induced pancreatitis. PAF plays a critical role in the regulation of progression of pancreatic injury and mediation of pancreatitis-associated lung injury in severe biliary pancreatitis. Amelioration of pancreatitis and prevention of pancreatitis-associated lung injury can be achieved with rPAF-AH even if treatment is begun after pancreatitis is established.
AB - Background and Aims: Recent reports suggest that platelet-activating factor (PAF) plays a role in pancreatitis and pancreatitis-associated lung injury. In this study, the effects on these processes of termination of PAF action by recombinant PAF-acetylhydrolase (rPAF-AH) were investigated. Methods: Rats were given rPAF-AH and then infused with a supramaximally stimulating dose of cerulein to induce mild pancreatitis. Opossums underwent biliopancreatic duct ligation to induce severe pancreatitis, and rPAF-AH administration was begun 2 days later. Results: In mild, secretagogue- induced pancreatitis, rPAF-AH given before the cerulein reduced hyperamylasemia, acinar cell vacuolization, and pancreatic inflammation but did not alter pancreatic edema or pulmonary microvascular permeability. In severe, biliopancreatic duct ligation-induced pancreatitis, rPAF-AH delayed and reduced the extent of inflammation and acinar cell injury/necrosis and completely prevented lung injury even though the rPAF-AH administration was begun after the onset of pancreatitis. Conclusions: PAF plays an important role in the regulation of pancreatic injury but not pancreatic edema or increased pulmonary microvascular permeability in mild, secretagogue-induced pancreatitis. PAF plays a critical role in the regulation of progression of pancreatic injury and mediation of pancreatitis-associated lung injury in severe biliary pancreatitis. Amelioration of pancreatitis and prevention of pancreatitis-associated lung injury can be achieved with rPAF-AH even if treatment is begun after pancreatitis is established.
UR - http://www.scopus.com/inward/record.url?scp=0031733230&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031733230&partnerID=8YFLogxK
U2 - 10.1016/S0016-5085(98)70096-4
DO - 10.1016/S0016-5085(98)70096-4
M3 - Article
C2 - 9797380
AN - SCOPUS:0031733230
VL - 115
SP - 1238
EP - 1247
JO - Gastroenterology
JF - Gastroenterology
SN - 0016-5085
IS - 5
ER -