Effect of recombinant vascular endothelial growth factor on experimental otitis media with effusion

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Conclusion. This study demonstrates that rVEGF in the middle ear cavity contributes significantly to the development of OME by increasing the vascular permeability of the middle ear mucosa. Objective. Vascular endothelial growth factor (VEGF) has been detected in otitis media with effusion (OME). It has a very strong effect on increasing vascular permeability, and is much more potent than platelet-activating factor, arachidonic acid metabolites or histamine. The purpose of this study was to investigate whether recombinant VEGF (rVEGF) can increase vascular permeability in the middle ear mucosa of rats. Material and methods. rVEGF was injected transtympanically at doses of 0.01, 0.1 and 1.0 μg in the experimental groups; normal saline was injected in the control group. Twenty-four h after the injection of rVEGF, middle ear fluid was aspirated and the vascular permeability of the middle ear mucosa was measured by means of the Evans Blue vital dye technique. Results. Extravasation of Evans Blue dye was found in all specimens injected with rVEGF, and was quantified using a spectrophotometer. Middle ear fluid developed in all ears injected with 0.1 or 1.0 μg of rVEGF and histopathology of the middle ear mucosa revealed severe inflammatory cellular infiltration, subepithelial edema and vascular dilatation. Rats injected with 1.0 μg of rVEGF showed a significant increase in middle ear vascular permeability in comparison with the control group (p <0.05).

Original languageEnglish
Pages (from-to)256-259
Number of pages4
JournalActa Oto-Laryngologica
Volume125
Issue number3
DOIs
Publication statusPublished - 2005 Apr 20

Fingerprint

Otitis Media with Effusion
Middle Ear
Vascular Endothelial Growth Factor A
Capillary Permeability
Mucous Membrane
Evans Blue
Coloring Agents
Control Groups
Platelet Activating Factor
Arachidonic Acid
Histamine
Ear
Blood Vessels
Dilatation
Edema
Injections

Keywords

  • Otitis
  • Pathogenesis
  • Rat
  • Vascular permeability

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Effect of recombinant vascular endothelial growth factor on experimental otitis media with effusion. / Kim, Tae-Hoon; Chae, Sungwon; Kim, Hyung Jin; Jung, Hak Hyun.

In: Acta Oto-Laryngologica, Vol. 125, No. 3, 20.04.2005, p. 256-259.

Research output: Contribution to journalArticle

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N2 - Conclusion. This study demonstrates that rVEGF in the middle ear cavity contributes significantly to the development of OME by increasing the vascular permeability of the middle ear mucosa. Objective. Vascular endothelial growth factor (VEGF) has been detected in otitis media with effusion (OME). It has a very strong effect on increasing vascular permeability, and is much more potent than platelet-activating factor, arachidonic acid metabolites or histamine. The purpose of this study was to investigate whether recombinant VEGF (rVEGF) can increase vascular permeability in the middle ear mucosa of rats. Material and methods. rVEGF was injected transtympanically at doses of 0.01, 0.1 and 1.0 μg in the experimental groups; normal saline was injected in the control group. Twenty-four h after the injection of rVEGF, middle ear fluid was aspirated and the vascular permeability of the middle ear mucosa was measured by means of the Evans Blue vital dye technique. Results. Extravasation of Evans Blue dye was found in all specimens injected with rVEGF, and was quantified using a spectrophotometer. Middle ear fluid developed in all ears injected with 0.1 or 1.0 μg of rVEGF and histopathology of the middle ear mucosa revealed severe inflammatory cellular infiltration, subepithelial edema and vascular dilatation. Rats injected with 1.0 μg of rVEGF showed a significant increase in middle ear vascular permeability in comparison with the control group (p <0.05).

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