TY - JOUR
T1 - Effect of red cyst cell inoculation and iron(II) supplementation on autotrophic astaxanthin production by Haematococcus pluvialis under outdoor summer conditions
AU - Hong, Min Eui
AU - Choi, Yoon Young
AU - Sim, Sang Jun
N1 - Funding Information:
This work was supported by the “ Energy Efficiency & Resources Technology R&D ” project (grant no. 20152010201900 ) of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea government Ministry of Trade, Industry & Energy (MOTIE) and supported by the National Research Foundation (NRF) grants (grant no. NRF-2013R1A2A1A01015644/2010-002795 5), University-Institute Cooperation Program (2013) and also supported by the Korea CCS R&D Center (KCRC) grant (grant no. 2014M1A8A1049278 ), funded by the Korean Government Ministry of Science, ICT & Future Planning (MSIP) .
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2016/1/20
Y1 - 2016/1/20
N2 - The negative effect of heat stress on the autotrophic astaxanthin production by Haematococcus pluvialis has been observed during outdoor culture in summer. Under the summer conditions, the proliferation of vegetative cells was highly halted in the green stage and the inducibility in the biosynthesis of astaxanthin was partly hindered in the red stage. Herein, under outdoor summer conditions in which variations of the diurnal temperature occur, heat-stress-driven inefficient vegetative growth of H. pluvialis was highly improved by inoculating the red cyst cells; thereby, maintaining relatively moderate intracellular carotenoid levels in the green stage. Subsequently, a remarkably enhanced astaxanthin titer was successfully obtained by supplementing 50μM iron(II) to induce the heat stress-driven Haber-Weiss reaction in the red stage. As a result, the productivity of astaxanthin in the cells cultured under summer temperature conditions (23.4-33.5°C) using the two methods of red cell (cyst) inoculation and the iron(Fe2+) supplementation was increased by 147% up to 5.53 mg/L day compared with that of the cells cultured under spring temperature conditions (17.5-27.3°C). Our technical solutions will definitely improve the annual natural astaxanthin productivity in H. pluvialis in locations confronted by hot summer weather, particularly in large-scale closed photobioreactor systems.
AB - The negative effect of heat stress on the autotrophic astaxanthin production by Haematococcus pluvialis has been observed during outdoor culture in summer. Under the summer conditions, the proliferation of vegetative cells was highly halted in the green stage and the inducibility in the biosynthesis of astaxanthin was partly hindered in the red stage. Herein, under outdoor summer conditions in which variations of the diurnal temperature occur, heat-stress-driven inefficient vegetative growth of H. pluvialis was highly improved by inoculating the red cyst cells; thereby, maintaining relatively moderate intracellular carotenoid levels in the green stage. Subsequently, a remarkably enhanced astaxanthin titer was successfully obtained by supplementing 50μM iron(II) to induce the heat stress-driven Haber-Weiss reaction in the red stage. As a result, the productivity of astaxanthin in the cells cultured under summer temperature conditions (23.4-33.5°C) using the two methods of red cell (cyst) inoculation and the iron(Fe2+) supplementation was increased by 147% up to 5.53 mg/L day compared with that of the cells cultured under spring temperature conditions (17.5-27.3°C). Our technical solutions will definitely improve the annual natural astaxanthin productivity in H. pluvialis in locations confronted by hot summer weather, particularly in large-scale closed photobioreactor systems.
KW - Astaxanthin
KW - Haematococcus pluvialis
KW - Iron(Fe) supplementation method
KW - Photoautotrophic culture
KW - Red cyst cell (aplanospore) inoculation method
KW - Summer outdoor conditions
UR - http://www.scopus.com/inward/record.url?scp=84949293192&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2015.11.019
DO - 10.1016/j.jbiotec.2015.11.019
M3 - Article
C2 - 26630998
AN - SCOPUS:84949293192
SN - 0168-1656
VL - 218
SP - 25
EP - 33
JO - Journal of Biotechnology
JF - Journal of Biotechnology
ER -