Effect of repeated seizure experiences on tyrosine hydroxylase immunoreactivities in the brain of genetically epilepsy-prone rats

Jae Ryun Ryu, Chan Young Shin, Kyu Hwan Park, Gye Sun Jeon, Hyoung Chun Kim, Won-Ki Kim, John W. Dailey, Phillip C. Jobe, Sa Sun Cho, Kwang Ho Ko

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The genetically epilepsy-prone rat (GEPR) is a model of generalized tonic/clonic epilepsy, and has functional noradrenergic deficiencies that act as partial determinants for the seizure predisposition and expression. The present study investigated the effect of repeated seizure experiences by acoustic stimulation (110 dB, 10 times) on the immunoreactivities of tyrosine hydroxylase (TH), a rate-determining enzyme in the synthesis of norepinephrine, in brain regions of GEPRs. TH immunoreactivity in locus coeruleus, the major noradrenergic nucleus in brain, was lower in GEPRs than control Sprague-Dawley rats. It was also decreased in several regions including inferior colliculus of GEPRs. Repeated experiences of audiogenic seizures further decreased TH immunoreactivities in locus coeruleus and inferior colliculus of GEPRs. The results from the present study suggest that the lower immunoreactivities of TH in locus coeruleus and inferior colliculus contribute, at least in part, to the noradrenergic deficits in GEPRs, and repeated seizure experiences further intensified these noradrenergic deficits, which may be related to the altered seizure expression by repetitive audiogenic seizure in GEPRs.

Original languageEnglish
Pages (from-to)777-782
Number of pages6
JournalBrain Research Bulletin
Volume53
Issue number6
DOIs
Publication statusPublished - 2000 Dec 1
Externally publishedYes

Fingerprint

Tyrosine 3-Monooxygenase
Epilepsy
Seizures
Inferior Colliculi
Locus Coeruleus
Brain
Tonic-Clonic Epilepsy
Acoustic Stimulation
Generalized Epilepsy
Sprague Dawley Rats
Norepinephrine
Enzymes

Keywords

  • Genetically epilepsy-prone rat
  • Immunoreactivity
  • Inferior colliculus
  • Locus coeruleus
  • Repeated audiogenic seizures
  • Tyrosine hydroxyalse

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Effect of repeated seizure experiences on tyrosine hydroxylase immunoreactivities in the brain of genetically epilepsy-prone rats. / Ryu, Jae Ryun; Shin, Chan Young; Park, Kyu Hwan; Jeon, Gye Sun; Kim, Hyoung Chun; Kim, Won-Ki; Dailey, John W.; Jobe, Phillip C.; Cho, Sa Sun; Ko, Kwang Ho.

In: Brain Research Bulletin, Vol. 53, No. 6, 01.12.2000, p. 777-782.

Research output: Contribution to journalArticle

Ryu, Jae Ryun ; Shin, Chan Young ; Park, Kyu Hwan ; Jeon, Gye Sun ; Kim, Hyoung Chun ; Kim, Won-Ki ; Dailey, John W. ; Jobe, Phillip C. ; Cho, Sa Sun ; Ko, Kwang Ho. / Effect of repeated seizure experiences on tyrosine hydroxylase immunoreactivities in the brain of genetically epilepsy-prone rats. In: Brain Research Bulletin. 2000 ; Vol. 53, No. 6. pp. 777-782.
@article{c9a18300f98a428dade72948933f3056,
title = "Effect of repeated seizure experiences on tyrosine hydroxylase immunoreactivities in the brain of genetically epilepsy-prone rats",
abstract = "The genetically epilepsy-prone rat (GEPR) is a model of generalized tonic/clonic epilepsy, and has functional noradrenergic deficiencies that act as partial determinants for the seizure predisposition and expression. The present study investigated the effect of repeated seizure experiences by acoustic stimulation (110 dB, 10 times) on the immunoreactivities of tyrosine hydroxylase (TH), a rate-determining enzyme in the synthesis of norepinephrine, in brain regions of GEPRs. TH immunoreactivity in locus coeruleus, the major noradrenergic nucleus in brain, was lower in GEPRs than control Sprague-Dawley rats. It was also decreased in several regions including inferior colliculus of GEPRs. Repeated experiences of audiogenic seizures further decreased TH immunoreactivities in locus coeruleus and inferior colliculus of GEPRs. The results from the present study suggest that the lower immunoreactivities of TH in locus coeruleus and inferior colliculus contribute, at least in part, to the noradrenergic deficits in GEPRs, and repeated seizure experiences further intensified these noradrenergic deficits, which may be related to the altered seizure expression by repetitive audiogenic seizure in GEPRs.",
keywords = "Genetically epilepsy-prone rat, Immunoreactivity, Inferior colliculus, Locus coeruleus, Repeated audiogenic seizures, Tyrosine hydroxyalse",
author = "Ryu, {Jae Ryun} and Shin, {Chan Young} and Park, {Kyu Hwan} and Jeon, {Gye Sun} and Kim, {Hyoung Chun} and Won-Ki Kim and Dailey, {John W.} and Jobe, {Phillip C.} and Cho, {Sa Sun} and Ko, {Kwang Ho}",
year = "2000",
month = "12",
day = "1",
doi = "10.1016/S0361-9230(00)00373-7",
language = "English",
volume = "53",
pages = "777--782",
journal = "Brain Research Bulletin",
issn = "0361-9230",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Effect of repeated seizure experiences on tyrosine hydroxylase immunoreactivities in the brain of genetically epilepsy-prone rats

AU - Ryu, Jae Ryun

AU - Shin, Chan Young

AU - Park, Kyu Hwan

AU - Jeon, Gye Sun

AU - Kim, Hyoung Chun

AU - Kim, Won-Ki

AU - Dailey, John W.

AU - Jobe, Phillip C.

AU - Cho, Sa Sun

AU - Ko, Kwang Ho

PY - 2000/12/1

Y1 - 2000/12/1

N2 - The genetically epilepsy-prone rat (GEPR) is a model of generalized tonic/clonic epilepsy, and has functional noradrenergic deficiencies that act as partial determinants for the seizure predisposition and expression. The present study investigated the effect of repeated seizure experiences by acoustic stimulation (110 dB, 10 times) on the immunoreactivities of tyrosine hydroxylase (TH), a rate-determining enzyme in the synthesis of norepinephrine, in brain regions of GEPRs. TH immunoreactivity in locus coeruleus, the major noradrenergic nucleus in brain, was lower in GEPRs than control Sprague-Dawley rats. It was also decreased in several regions including inferior colliculus of GEPRs. Repeated experiences of audiogenic seizures further decreased TH immunoreactivities in locus coeruleus and inferior colliculus of GEPRs. The results from the present study suggest that the lower immunoreactivities of TH in locus coeruleus and inferior colliculus contribute, at least in part, to the noradrenergic deficits in GEPRs, and repeated seizure experiences further intensified these noradrenergic deficits, which may be related to the altered seizure expression by repetitive audiogenic seizure in GEPRs.

AB - The genetically epilepsy-prone rat (GEPR) is a model of generalized tonic/clonic epilepsy, and has functional noradrenergic deficiencies that act as partial determinants for the seizure predisposition and expression. The present study investigated the effect of repeated seizure experiences by acoustic stimulation (110 dB, 10 times) on the immunoreactivities of tyrosine hydroxylase (TH), a rate-determining enzyme in the synthesis of norepinephrine, in brain regions of GEPRs. TH immunoreactivity in locus coeruleus, the major noradrenergic nucleus in brain, was lower in GEPRs than control Sprague-Dawley rats. It was also decreased in several regions including inferior colliculus of GEPRs. Repeated experiences of audiogenic seizures further decreased TH immunoreactivities in locus coeruleus and inferior colliculus of GEPRs. The results from the present study suggest that the lower immunoreactivities of TH in locus coeruleus and inferior colliculus contribute, at least in part, to the noradrenergic deficits in GEPRs, and repeated seizure experiences further intensified these noradrenergic deficits, which may be related to the altered seizure expression by repetitive audiogenic seizure in GEPRs.

KW - Genetically epilepsy-prone rat

KW - Immunoreactivity

KW - Inferior colliculus

KW - Locus coeruleus

KW - Repeated audiogenic seizures

KW - Tyrosine hydroxyalse

UR - http://www.scopus.com/inward/record.url?scp=0034496615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034496615&partnerID=8YFLogxK

U2 - 10.1016/S0361-9230(00)00373-7

DO - 10.1016/S0361-9230(00)00373-7

M3 - Article

VL - 53

SP - 777

EP - 782

JO - Brain Research Bulletin

JF - Brain Research Bulletin

SN - 0361-9230

IS - 6

ER -