Effect of spa spring water on cytokine expression in human keratinocyte HaCaT cells and on differentiation of CD4 + T cells

Ho Pyo Lee, Yoon Jung Choi, Kyung Ah Cho, So Youn Woo, Seong Taek Yun, Jong Tae Lee, Hong Jig Kim, Kyung Ho Lee, Jin Wou Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Skin acts as the first line of defense against any foreign materials outside of our body. In inflammatory skin disease, the pathogenesis is due to an immune reaction in the keratinocytes, immune cells and soluble mediators. Balneotherapy is widely used for the treatment of inflammatory skin disease, but the mechanisms are only partly understood by immune regulation. Balneotherapy in dermatologic disease can affect the secretion of pro- inflammatory cytokines, IL-1 α and tumor necrosis factor from keratinocytes, and possibly affect the T cell differentiation. Objective: In this study, we evaluated the effect of spa spring water from Yong-gung oncheon on the cells, and investigated the skin immune reaction. Methods: We investigated the immunomodulatory or anti-inflammatory effect of thermal spring water on the expression of pro-inflammatory cytokines in the HaCaT cells under Toll-like receptor (TLR) stimulation, as well as the effect on the differentiation of CD4 + T cells under spring water. Results: The treatment of spa spring water from Yong-gung oncheon decreased the expression of proinflammatory cytokines under TLR stimulation to the HaCaT cells and antigen presenting cells. In addition, spa spring water attenuated the differentiation process of subsets of CD4 + T cells, i.e., Th1, Th2 and Th17 cells. All these immune parameters can be used to evaluate the efficacy of spa spring water in Korea, in terms of the immune modulatory effect. Conclusion: Spa spring water treatment suppressed the inflammatory cytokines production and also modulated the differentiation of CD4 + T cells into Th1, Th2, and Th17 cells, but not the Tregs cells. (Ann Dermatol 24(3) 324~336, 2012).

Original languageEnglish
Pages (from-to)324-336
Number of pages13
JournalAnnals of Dermatology
Volume24
Issue number3
DOIs
Publication statusPublished - 2012 Aug 1

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Keratinocytes
Cell Differentiation
Cytokines
T-Lymphocytes
Water
Balneology
Th17 Cells
Th2 Cells
Th1 Cells
Toll-Like Receptors
Skin Diseases
Skin
Water Purification
T-Lymphocyte Subsets
Antigen-Presenting Cells
Korea
Interleukin-1
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Hot Temperature

Keywords

  • Balneotherapy
  • CD4+ T cells
  • Cytokines
  • HaCaT cells
  • Skin inflammation

ASJC Scopus subject areas

  • Dermatology

Cite this

Effect of spa spring water on cytokine expression in human keratinocyte HaCaT cells and on differentiation of CD4 + T cells. / Lee, Ho Pyo; Choi, Yoon Jung; Cho, Kyung Ah; Woo, So Youn; Yun, Seong Taek; Lee, Jong Tae; Kim, Hong Jig; Lee, Kyung Ho; Kim, Jin Wou.

In: Annals of Dermatology, Vol. 24, No. 3, 01.08.2012, p. 324-336.

Research output: Contribution to journalArticle

Lee, Ho Pyo ; Choi, Yoon Jung ; Cho, Kyung Ah ; Woo, So Youn ; Yun, Seong Taek ; Lee, Jong Tae ; Kim, Hong Jig ; Lee, Kyung Ho ; Kim, Jin Wou. / Effect of spa spring water on cytokine expression in human keratinocyte HaCaT cells and on differentiation of CD4 + T cells. In: Annals of Dermatology. 2012 ; Vol. 24, No. 3. pp. 324-336.
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abstract = "Background: Skin acts as the first line of defense against any foreign materials outside of our body. In inflammatory skin disease, the pathogenesis is due to an immune reaction in the keratinocytes, immune cells and soluble mediators. Balneotherapy is widely used for the treatment of inflammatory skin disease, but the mechanisms are only partly understood by immune regulation. Balneotherapy in dermatologic disease can affect the secretion of pro- inflammatory cytokines, IL-1 α and tumor necrosis factor from keratinocytes, and possibly affect the T cell differentiation. Objective: In this study, we evaluated the effect of spa spring water from Yong-gung oncheon on the cells, and investigated the skin immune reaction. Methods: We investigated the immunomodulatory or anti-inflammatory effect of thermal spring water on the expression of pro-inflammatory cytokines in the HaCaT cells under Toll-like receptor (TLR) stimulation, as well as the effect on the differentiation of CD4 + T cells under spring water. Results: The treatment of spa spring water from Yong-gung oncheon decreased the expression of proinflammatory cytokines under TLR stimulation to the HaCaT cells and antigen presenting cells. In addition, spa spring water attenuated the differentiation process of subsets of CD4 + T cells, i.e., Th1, Th2 and Th17 cells. All these immune parameters can be used to evaluate the efficacy of spa spring water in Korea, in terms of the immune modulatory effect. Conclusion: Spa spring water treatment suppressed the inflammatory cytokines production and also modulated the differentiation of CD4 + T cells into Th1, Th2, and Th17 cells, but not the Tregs cells. (Ann Dermatol 24(3) 324~336, 2012).",
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AU - Lee, Ho Pyo

AU - Choi, Yoon Jung

AU - Cho, Kyung Ah

AU - Woo, So Youn

AU - Yun, Seong Taek

AU - Lee, Jong Tae

AU - Kim, Hong Jig

AU - Lee, Kyung Ho

AU - Kim, Jin Wou

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N2 - Background: Skin acts as the first line of defense against any foreign materials outside of our body. In inflammatory skin disease, the pathogenesis is due to an immune reaction in the keratinocytes, immune cells and soluble mediators. Balneotherapy is widely used for the treatment of inflammatory skin disease, but the mechanisms are only partly understood by immune regulation. Balneotherapy in dermatologic disease can affect the secretion of pro- inflammatory cytokines, IL-1 α and tumor necrosis factor from keratinocytes, and possibly affect the T cell differentiation. Objective: In this study, we evaluated the effect of spa spring water from Yong-gung oncheon on the cells, and investigated the skin immune reaction. Methods: We investigated the immunomodulatory or anti-inflammatory effect of thermal spring water on the expression of pro-inflammatory cytokines in the HaCaT cells under Toll-like receptor (TLR) stimulation, as well as the effect on the differentiation of CD4 + T cells under spring water. Results: The treatment of spa spring water from Yong-gung oncheon decreased the expression of proinflammatory cytokines under TLR stimulation to the HaCaT cells and antigen presenting cells. In addition, spa spring water attenuated the differentiation process of subsets of CD4 + T cells, i.e., Th1, Th2 and Th17 cells. All these immune parameters can be used to evaluate the efficacy of spa spring water in Korea, in terms of the immune modulatory effect. Conclusion: Spa spring water treatment suppressed the inflammatory cytokines production and also modulated the differentiation of CD4 + T cells into Th1, Th2, and Th17 cells, but not the Tregs cells. (Ann Dermatol 24(3) 324~336, 2012).

AB - Background: Skin acts as the first line of defense against any foreign materials outside of our body. In inflammatory skin disease, the pathogenesis is due to an immune reaction in the keratinocytes, immune cells and soluble mediators. Balneotherapy is widely used for the treatment of inflammatory skin disease, but the mechanisms are only partly understood by immune regulation. Balneotherapy in dermatologic disease can affect the secretion of pro- inflammatory cytokines, IL-1 α and tumor necrosis factor from keratinocytes, and possibly affect the T cell differentiation. Objective: In this study, we evaluated the effect of spa spring water from Yong-gung oncheon on the cells, and investigated the skin immune reaction. Methods: We investigated the immunomodulatory or anti-inflammatory effect of thermal spring water on the expression of pro-inflammatory cytokines in the HaCaT cells under Toll-like receptor (TLR) stimulation, as well as the effect on the differentiation of CD4 + T cells under spring water. Results: The treatment of spa spring water from Yong-gung oncheon decreased the expression of proinflammatory cytokines under TLR stimulation to the HaCaT cells and antigen presenting cells. In addition, spa spring water attenuated the differentiation process of subsets of CD4 + T cells, i.e., Th1, Th2 and Th17 cells. All these immune parameters can be used to evaluate the efficacy of spa spring water in Korea, in terms of the immune modulatory effect. Conclusion: Spa spring water treatment suppressed the inflammatory cytokines production and also modulated the differentiation of CD4 + T cells into Th1, Th2, and Th17 cells, but not the Tregs cells. (Ann Dermatol 24(3) 324~336, 2012).

KW - Balneotherapy

KW - CD4+ T cells

KW - Cytokines

KW - HaCaT cells

KW - Skin inflammation

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