Effect of specific production rate of recombinant protein on multimerization of plasmid vector and gene expression level

Vibhor Saraswat, Dae Young Kim, Jeewon Lee, Young Hoon Park

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In fed-batch cultures of recombinant Escherichia coli BL21(DE3)[pT7-G3IL2] at high cell concentration, the post-induction specific growth rate was carefully regulated by controlled medium feed to maximize the synthesis level of recombinant fusion interleukin-2, G3.IL-2. A maximum concentration of G3.IL-2 (11.25 g l-1) was achieved in the induced recombinant culture growing at the rate of 0.056 h-1. A steep decrease in the expression level of G3.IL-2 was observed at the post-induction specific growth rates higher than its optimal value (0.056 h-1). In the induced recombinant cultures, plasmid multimerization was observed and highly dependent on specific growth and production rate: a higher post-induction specific growth rate and an increased specific production rate tended to significantly promote it much further. Moreover, plasmid stability was found to decrease rapidly in a faster growing culture. Copyright (C) 1999 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)367-373
Number of pages7
JournalFEMS Microbiology Letters
Volume179
Issue number2
DOIs
Publication statusPublished - 1999 Oct 15
Externally publishedYes

Keywords

  • Escherichia coli
  • Plasmid multimerization
  • Recombinant human interleukin-2
  • Specific production rate

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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