Effect of substrate concentration on the competition between Clostridium and Lactobacillus during biohydrogen production

Jeong Hoon Park, Do Hyung Kim, Sang Hyoun Kim, Jeong Jun Yoon, Hee-Deung Park

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To evaluate the effect of substrate concentration on competition between hydrogen producing bacteria and lactic acid bacteria during hydrogen fermentation, a series of batch tests was conducted using galactose or glucose as a carbon source. Clostridium butyricum, Lactobacillus casei, and mixture of the two species were used for inoculum, respectively. The batch tests based on single species revealed that C. butyricum utilized galactose faster than L. casei, regardless of substrate concentration (0.2-5.7 g/L), while L. casei utilized glucose faster than C. butyricum at low levels of glucose (<1 g/L). These trends were also observed in the batch test based on a mixture of the two species. L. casei outcompeted C. butyricum only when glucose was provided at low concentration. Taken together, the results suggest that failure of biohydrogen production can occur at low glucose concentration via outcompeting of lactic acid bacteria over hydrogen producing bacteria.

Original languageEnglish
JournalInternational Journal of Hydrogen Energy
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Clostridium
glucose
Glucose
bacteria
Bacteria
Substrates
galactose
lactic acid
Lactic acid
Hydrogen
hydrogen
inoculum
fermentation
Fermentation
low concentrations
trends
acids
Carbon
carbon

Keywords

  • Biohydrogen
  • Clostridium
  • Lactobacillus
  • Organic acids
  • Substrate competition
  • Substrate concentration

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

Effect of substrate concentration on the competition between Clostridium and Lactobacillus during biohydrogen production. / Park, Jeong Hoon; Kim, Do Hyung; Kim, Sang Hyoun; Yoon, Jeong Jun; Park, Hee-Deung.

In: International Journal of Hydrogen Energy, 2017.

Research output: Contribution to journalArticle

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N2 - To evaluate the effect of substrate concentration on competition between hydrogen producing bacteria and lactic acid bacteria during hydrogen fermentation, a series of batch tests was conducted using galactose or glucose as a carbon source. Clostridium butyricum, Lactobacillus casei, and mixture of the two species were used for inoculum, respectively. The batch tests based on single species revealed that C. butyricum utilized galactose faster than L. casei, regardless of substrate concentration (0.2-5.7 g/L), while L. casei utilized glucose faster than C. butyricum at low levels of glucose (<1 g/L). These trends were also observed in the batch test based on a mixture of the two species. L. casei outcompeted C. butyricum only when glucose was provided at low concentration. Taken together, the results suggest that failure of biohydrogen production can occur at low glucose concentration via outcompeting of lactic acid bacteria over hydrogen producing bacteria.

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