Effect of TCDD on corpus cavernosum histology and smooth muscle physiology

D. G. Moon; K. C. Lee; Y. W. Kim; H. S. Park; H. Y. Cho; J. J. Kim

doi:10.1038/sj.ijir.3901060

Effect of TCDD on corpus cavernosum histology and smooth muscle physiology

D. G. Moon, K. C. Lee, Y. W. Kim, H. S. Park, H. Y. Cho, J. J. Kim

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

A polyhalogenated aromatic hydrocarbon, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is one of the most potent toxic environmental pollutants. Decreases in spermatogenesis and the ability to conceive and carry a pregnancy to term are the most sensitive signs of reproductive toxicity by TCDD in the mammal, but no report of its effect on the erectile function exists. We performed this study to investigate the effect of TCDD on the erectile function. New Zealand white rabbits were treated intraperitoneally with 1 μg/kg of TCDD. At 4 (Gr I) and 8 (Gr II) weeks after the administration of TCDD, cavernosal tissues were harvested for strip study in the organ bath and testes were prepared for histologic examination. Compared to the maximal amplitude of 17.1 ± 4.12 mN in normal control (Gr III), the contractions to cumulative concentrations of NE (10^-8-10^-4 M) were significantly decreased to 6.57 ± 1.34 and 5.45 ± 1.01 mN in Groups I and II, respectively. Compared to 51.12 ± 7.38% in Gr III, relaxation to cumulative concentration (10^-8-10^-4 M) of acetylcholine was significantly decreased to 17.25 ± 2.17% (Gr I) and 9.73 ± 2.17% (Gr II) at a concentration of 10^-4 M, respectively. Compared to 75.12 ± 13.18% in Gr III, relaxation to cumulative concentration (10 ^-8-10^-4 M) of SNP was significantly decreased to 31.49 + 7.89% (Gr I) and 18.54 ± 6.12% (Gr II) at a concentration of 10 ^-4 M, respectively. Histologically, intracavernosal fibrosis, abnormal subtunical deposition of fat and decreased sinusoidal space with consequent increase of trabecular smooth muscle contents were identified in TCDD-treated groups. In TCDD-treated animals, seminiferous tubules showed a decrease of germ cells with vacuolar degeneration and apoptotic cells. Spermatids were hardly seen. These results suggest that TCDD inhibits spermatogenesis and has a potential harmful effect on erectile function via changes of corpus cavernosum histology and smooth muscle physiology.

Original language	English
Pages (from-to)	224-230
Number of pages	7
Journal	International Journal of Impotence Research
Volume	16
Issue number	3
DOIs	https://doi.org/10.1038/sj.ijir.3901060
Publication status	Published - 2004 Jun
Externally published	Yes

Keywords

Erection
Penis
TCDD

ASJC Scopus subject areas

Urology

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10.1038/sj.ijir.3901060

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@article{3c55a58a3fca465b86931d7a5ceb4728,

title = "Effect of TCDD on corpus cavernosum histology and smooth muscle physiology",

abstract = "A polyhalogenated aromatic hydrocarbon, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is one of the most potent toxic environmental pollutants. Decreases in spermatogenesis and the ability to conceive and carry a pregnancy to term are the most sensitive signs of reproductive toxicity by TCDD in the mammal, but no report of its effect on the erectile function exists. We performed this study to investigate the effect of TCDD on the erectile function. New Zealand white rabbits were treated intraperitoneally with 1 μg/kg of TCDD. At 4 (Gr I) and 8 (Gr II) weeks after the administration of TCDD, cavernosal tissues were harvested for strip study in the organ bath and testes were prepared for histologic examination. Compared to the maximal amplitude of 17.1 ± 4.12 mN in normal control (Gr III), the contractions to cumulative concentrations of NE (10-8-10-4 M) were significantly decreased to 6.57 ± 1.34 and 5.45 ± 1.01 mN in Groups I and II, respectively. Compared to 51.12 ± 7.38% in Gr III, relaxation to cumulative concentration (10-8-10-4 M) of acetylcholine was significantly decreased to 17.25 ± 2.17% (Gr I) and 9.73 ± 2.17% (Gr II) at a concentration of 10-4 M, respectively. Compared to 75.12 ± 13.18% in Gr III, relaxation to cumulative concentration (10 -8-10-4 M) of SNP was significantly decreased to 31.49 + 7.89% (Gr I) and 18.54 ± 6.12% (Gr II) at a concentration of 10 -4 M, respectively. Histologically, intracavernosal fibrosis, abnormal subtunical deposition of fat and decreased sinusoidal space with consequent increase of trabecular smooth muscle contents were identified in TCDD-treated groups. In TCDD-treated animals, seminiferous tubules showed a decrease of germ cells with vacuolar degeneration and apoptotic cells. Spermatids were hardly seen. These results suggest that TCDD inhibits spermatogenesis and has a potential harmful effect on erectile function via changes of corpus cavernosum histology and smooth muscle physiology.",

keywords = "Erection, Penis, TCDD",

author = "Moon, {D. G.} and Lee, {K. C.} and Kim, {Y. W.} and Park, {H. S.} and Cho, {H. Y.} and Kim, {J. J.}",

year = "2004",

month = jun,

doi = "10.1038/sj.ijir.3901060",

language = "English",

volume = "16",

pages = "224--230",

journal = "International Journal of Impotence Research",

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T1 - Effect of TCDD on corpus cavernosum histology and smooth muscle physiology

AU - Moon, D. G.

AU - Lee, K. C.

AU - Kim, Y. W.

AU - Park, H. S.

AU - Cho, H. Y.

AU - Kim, J. J.

PY - 2004/6

Y1 - 2004/6

N2 - A polyhalogenated aromatic hydrocarbon, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is one of the most potent toxic environmental pollutants. Decreases in spermatogenesis and the ability to conceive and carry a pregnancy to term are the most sensitive signs of reproductive toxicity by TCDD in the mammal, but no report of its effect on the erectile function exists. We performed this study to investigate the effect of TCDD on the erectile function. New Zealand white rabbits were treated intraperitoneally with 1 μg/kg of TCDD. At 4 (Gr I) and 8 (Gr II) weeks after the administration of TCDD, cavernosal tissues were harvested for strip study in the organ bath and testes were prepared for histologic examination. Compared to the maximal amplitude of 17.1 ± 4.12 mN in normal control (Gr III), the contractions to cumulative concentrations of NE (10-8-10-4 M) were significantly decreased to 6.57 ± 1.34 and 5.45 ± 1.01 mN in Groups I and II, respectively. Compared to 51.12 ± 7.38% in Gr III, relaxation to cumulative concentration (10-8-10-4 M) of acetylcholine was significantly decreased to 17.25 ± 2.17% (Gr I) and 9.73 ± 2.17% (Gr II) at a concentration of 10-4 M, respectively. Compared to 75.12 ± 13.18% in Gr III, relaxation to cumulative concentration (10 -8-10-4 M) of SNP was significantly decreased to 31.49 + 7.89% (Gr I) and 18.54 ± 6.12% (Gr II) at a concentration of 10 -4 M, respectively. Histologically, intracavernosal fibrosis, abnormal subtunical deposition of fat and decreased sinusoidal space with consequent increase of trabecular smooth muscle contents were identified in TCDD-treated groups. In TCDD-treated animals, seminiferous tubules showed a decrease of germ cells with vacuolar degeneration and apoptotic cells. Spermatids were hardly seen. These results suggest that TCDD inhibits spermatogenesis and has a potential harmful effect on erectile function via changes of corpus cavernosum histology and smooth muscle physiology.

AB - A polyhalogenated aromatic hydrocarbon, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is one of the most potent toxic environmental pollutants. Decreases in spermatogenesis and the ability to conceive and carry a pregnancy to term are the most sensitive signs of reproductive toxicity by TCDD in the mammal, but no report of its effect on the erectile function exists. We performed this study to investigate the effect of TCDD on the erectile function. New Zealand white rabbits were treated intraperitoneally with 1 μg/kg of TCDD. At 4 (Gr I) and 8 (Gr II) weeks after the administration of TCDD, cavernosal tissues were harvested for strip study in the organ bath and testes were prepared for histologic examination. Compared to the maximal amplitude of 17.1 ± 4.12 mN in normal control (Gr III), the contractions to cumulative concentrations of NE (10-8-10-4 M) were significantly decreased to 6.57 ± 1.34 and 5.45 ± 1.01 mN in Groups I and II, respectively. Compared to 51.12 ± 7.38% in Gr III, relaxation to cumulative concentration (10-8-10-4 M) of acetylcholine was significantly decreased to 17.25 ± 2.17% (Gr I) and 9.73 ± 2.17% (Gr II) at a concentration of 10-4 M, respectively. Compared to 75.12 ± 13.18% in Gr III, relaxation to cumulative concentration (10 -8-10-4 M) of SNP was significantly decreased to 31.49 + 7.89% (Gr I) and 18.54 ± 6.12% (Gr II) at a concentration of 10 -4 M, respectively. Histologically, intracavernosal fibrosis, abnormal subtunical deposition of fat and decreased sinusoidal space with consequent increase of trabecular smooth muscle contents were identified in TCDD-treated groups. In TCDD-treated animals, seminiferous tubules showed a decrease of germ cells with vacuolar degeneration and apoptotic cells. Spermatids were hardly seen. These results suggest that TCDD inhibits spermatogenesis and has a potential harmful effect on erectile function via changes of corpus cavernosum histology and smooth muscle physiology.

KW - Erection

KW - Penis

KW - TCDD

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M3 - Article

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AN - SCOPUS:3042740944

VL - 16

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JO - International Journal of Impotence Research

JF - International Journal of Impotence Research

SN - 0955-9930

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ER -

Effect of TCDD on corpus cavernosum histology and smooth muscle physiology

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