Effect of the supplement of metabolites on cell growth and poly-β-hydroxybutyrate biosynthesis of Alcaligenes latus

Yong Hyun Lee, Tae Woo Kim, Jin Seo Park, Tae Lin Huh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The characteristics of cell growth and poly-β-hydroxybutyrate biosynthesis of Alcaligenes latus ATCC 29713 were investigated. The PHB accumulation pattern of A. latus followed a growth-associated type where the cell growth and PHB accumulation were carried out simultaneously. Various intermediate compounds such as metabolites involved in the TCA cycle, amino acids, and saturated and unsaturated fatty acids were added to examine their effect on cell growth and PHB accumulation. Citrate, tyrosine, and pal-mitic acid showed the most significant increase both on cell growth and PHB accumulation. Maximum PHB concentrations were noticeably increased about 1.4 to 1.6 times higher than that of control, corresponding to 5.54, 6.45, and 6.45 g/l for citrate, tyrosine, and palmitic acid, respectively. The stimulatory effects of the supplemented metabolites were analyzed in terms of the increment of enzyme activities related to sugar catabolism and PHB biosynthesis.

Original languageEnglish
Pages (from-to)120-127
Number of pages8
JournalJournal of Microbiology and Biotechnology
Volume6
Issue number2
Publication statusPublished - 1996 Apr 1
Externally publishedYes

Fingerprint

Hydroxybutyrates
Alcaligenes
Biosynthesis
Cell growth
Metabolites
Growth
Citric Acid
Tyrosine
Saturated fatty acids
Unsaturated fatty acids
Palmitic acid
Palmitic Acid
Enzyme activity
Unsaturated Fatty Acids
Sugars
Amino acids
Fatty Acids
Amino Acids
Acids
Enzymes

Keywords

  • Alcaligenes latus
  • Amino acids
  • Cell growth
  • Fatty acids
  • Intermediate compound
  • Intermediates of TCA cycle
  • Poly-β-hydroxybutyrate

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Effect of the supplement of metabolites on cell growth and poly-β-hydroxybutyrate biosynthesis of Alcaligenes latus. / Lee, Yong Hyun; Kim, Tae Woo; Park, Jin Seo; Huh, Tae Lin.

In: Journal of Microbiology and Biotechnology, Vol. 6, No. 2, 01.04.1996, p. 120-127.

Research output: Contribution to journalArticle

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