Effect of urban particles on human middle ear epithelial cells

Gi Jung Im, Moo Kyun Park, Jae-Jun Song

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objectives: The aim of this study was to examine the cytotoxic effect and inflammatory response of human middle ear epithelial cells (HMEECs) induced by urban particles (UP). Materials and methods: Cell viability following UP exposure was assessed in HMEECs using the CCK 8 assay. The expression levels of the inflammation-related genes (COX-2 and MUC5AC) were analyzed using semi-quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and COX-2 production was analyzed using western blotting. Results: Treatment with UP decreased cell viability in HMEECs in a dose- and time-dependent manner. UP exposure induced the significantly increased expression of COX-2 and MUC5AC. Western blotting showed dose dependently increased expression of COX-2 production. Conclusions: UP decreased cell viability, increased the inflammatory response, and increased mucin gene production in HMEECs. These findings indicate that exposure to UP can contribute to the development of otitis media.

Original languageEnglish
Pages (from-to)777-781
Number of pages5
JournalInternational Journal of Pediatric Otorhinolaryngology
Volume78
Issue number5
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Middle Ear
Epithelial Cells
Cell Survival
Western Blotting
Sincalide
Otitis Media
Mucins
Reverse Transcriptase Polymerase Chain Reaction
Genes
Real-Time Polymerase Chain Reaction
Inflammation

Keywords

  • Cyclooxygenase 2
  • Human middle ear epithelial cells
  • MUC5AC
  • Otitis media
  • Urban particles

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Otorhinolaryngology

Cite this

Effect of urban particles on human middle ear epithelial cells. / Im, Gi Jung; Park, Moo Kyun; Song, Jae-Jun.

In: International Journal of Pediatric Otorhinolaryngology, Vol. 78, No. 5, 01.01.2014, p. 777-781.

Research output: Contribution to journalArticle

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