Abstract
During the process of bone formation, titanium (Ti) surface is an important factor in the modulation of osteoblastic function. This study was conducted in order to determine the effects of different Ti surfaces on the biological responses of a human osteoblast-like cell line (MG63). MG63 cells were cultured on smooth (S), sandblasted large-grit and acid etching (SLA), hydroxyapatite (HA), hydroxyfluoride (HF), titanium nitrate (TIN), and diamond-like carbon (DLC) Ti. The morphology of these cells were assessed by SEM. The cDNAs prepared from the total RNAs of the MG63 were hybridized into a human cDNA microarray (1152 elements). The appearances of the surfaces observed by SEM were different on each of the six dental substrate types. The SLA and HA surfaces were determined to be rougher than the others. MG63 cells cultured on SLA and HA exhibited cell-matrix interactions. In the expression of genes involved in osseointegration, several genes, including bone morphogenetic protein, cadherin, integrin, and insulin-like growth factors, were upregulated on the different surfaces. Several genes, including fibroblast growth factor receptor 4, Bcl 2-related protein, and collagen, were downregulated on the different surfaces. The attachment and expression of key osteogenic regulatory genes were enhanced by the surface roughness of the dental materials used.
| Original language | English |
|---|---|
| Pages (from-to) | 368-379 |
| Number of pages | 12 |
| Journal | Journal of Oral Rehabilitation |
| Volume | 33 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2006 May 1 |
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Keywords
- cDNA microarray
- Cell morphology
- Dental titanium surfaces
- Expression profiling
- Gene expression
- Gene profiling
ASJC Scopus subject areas
- Dentistry(all)
Cite this
Effect of various implant coatings on biological responses in MG63 using cDNA microarray. / Kim, C. S.; Sohn, S. H.; Jeon, S. K.; Kim, K. N.; Ryu, Jae Jun; Kim, Meyoung-Kon.
In: Journal of Oral Rehabilitation, Vol. 33, No. 5, 01.05.2006, p. 368-379.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of various implant coatings on biological responses in MG63 using cDNA microarray
AU - Kim, C. S.
AU - Sohn, S. H.
AU - Jeon, S. K.
AU - Kim, K. N.
AU - Ryu, Jae Jun
AU - Kim, Meyoung-Kon
PY - 2006/5/1
Y1 - 2006/5/1
N2 - During the process of bone formation, titanium (Ti) surface is an important factor in the modulation of osteoblastic function. This study was conducted in order to determine the effects of different Ti surfaces on the biological responses of a human osteoblast-like cell line (MG63). MG63 cells were cultured on smooth (S), sandblasted large-grit and acid etching (SLA), hydroxyapatite (HA), hydroxyfluoride (HF), titanium nitrate (TIN), and diamond-like carbon (DLC) Ti. The morphology of these cells were assessed by SEM. The cDNAs prepared from the total RNAs of the MG63 were hybridized into a human cDNA microarray (1152 elements). The appearances of the surfaces observed by SEM were different on each of the six dental substrate types. The SLA and HA surfaces were determined to be rougher than the others. MG63 cells cultured on SLA and HA exhibited cell-matrix interactions. In the expression of genes involved in osseointegration, several genes, including bone morphogenetic protein, cadherin, integrin, and insulin-like growth factors, were upregulated on the different surfaces. Several genes, including fibroblast growth factor receptor 4, Bcl 2-related protein, and collagen, were downregulated on the different surfaces. The attachment and expression of key osteogenic regulatory genes were enhanced by the surface roughness of the dental materials used.
AB - During the process of bone formation, titanium (Ti) surface is an important factor in the modulation of osteoblastic function. This study was conducted in order to determine the effects of different Ti surfaces on the biological responses of a human osteoblast-like cell line (MG63). MG63 cells were cultured on smooth (S), sandblasted large-grit and acid etching (SLA), hydroxyapatite (HA), hydroxyfluoride (HF), titanium nitrate (TIN), and diamond-like carbon (DLC) Ti. The morphology of these cells were assessed by SEM. The cDNAs prepared from the total RNAs of the MG63 were hybridized into a human cDNA microarray (1152 elements). The appearances of the surfaces observed by SEM were different on each of the six dental substrate types. The SLA and HA surfaces were determined to be rougher than the others. MG63 cells cultured on SLA and HA exhibited cell-matrix interactions. In the expression of genes involved in osseointegration, several genes, including bone morphogenetic protein, cadherin, integrin, and insulin-like growth factors, were upregulated on the different surfaces. Several genes, including fibroblast growth factor receptor 4, Bcl 2-related protein, and collagen, were downregulated on the different surfaces. The attachment and expression of key osteogenic regulatory genes were enhanced by the surface roughness of the dental materials used.
KW - cDNA microarray
KW - Cell morphology
KW - Dental titanium surfaces
KW - Expression profiling
KW - Gene expression
KW - Gene profiling
UR - http://www.scopus.com/inward/record.url?scp=33645886902&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645886902&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2842.2005.01553.x
DO - 10.1111/j.1365-2842.2005.01553.x
M3 - Article
C2 - 16629896
AN - SCOPUS:33645886902
VL - 33
SP - 368
EP - 379
JO - Journal of Oral Rehabilitation
JF - Journal of Oral Rehabilitation
SN - 0305-182X
IS - 5
ER -