Effect of various implant coatings on biological responses in MG63 using cDNA microarray

C. S. Kim, S. H. Sohn, S. K. Jeon, K. N. Kim, Jae Jun Ryu, Meyoung-Kon Kim

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

During the process of bone formation, titanium (Ti) surface is an important factor in the modulation of osteoblastic function. This study was conducted in order to determine the effects of different Ti surfaces on the biological responses of a human osteoblast-like cell line (MG63). MG63 cells were cultured on smooth (S), sandblasted large-grit and acid etching (SLA), hydroxyapatite (HA), hydroxyfluoride (HF), titanium nitrate (TIN), and diamond-like carbon (DLC) Ti. The morphology of these cells were assessed by SEM. The cDNAs prepared from the total RNAs of the MG63 were hybridized into a human cDNA microarray (1152 elements). The appearances of the surfaces observed by SEM were different on each of the six dental substrate types. The SLA and HA surfaces were determined to be rougher than the others. MG63 cells cultured on SLA and HA exhibited cell-matrix interactions. In the expression of genes involved in osseointegration, several genes, including bone morphogenetic protein, cadherin, integrin, and insulin-like growth factors, were upregulated on the different surfaces. Several genes, including fibroblast growth factor receptor 4, Bcl 2-related protein, and collagen, were downregulated on the different surfaces. The attachment and expression of key osteogenic regulatory genes were enhanced by the surface roughness of the dental materials used.

Original languageEnglish
Pages (from-to)368-379
Number of pages12
JournalJournal of Oral Rehabilitation
Volume33
Issue number5
DOIs
Publication statusPublished - 2006 May 1

Fingerprint

Titanium
Oligonucleotide Array Sequence Analysis
Durapatite
Acids
Receptor, Fibroblast Growth Factor, Type 4
Cultured Cells
Osseointegration
Dental Materials
Bone Morphogenetic Proteins
Diamond
Somatomedins
Cadherins
Regulator Genes
Osteoblasts
Osteogenesis
Integrins
Cell Communication
Nitrates
Genes
Tooth

Keywords

  • cDNA microarray
  • Cell morphology
  • Dental titanium surfaces
  • Expression profiling
  • Gene expression
  • Gene profiling

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effect of various implant coatings on biological responses in MG63 using cDNA microarray. / Kim, C. S.; Sohn, S. H.; Jeon, S. K.; Kim, K. N.; Ryu, Jae Jun; Kim, Meyoung-Kon.

In: Journal of Oral Rehabilitation, Vol. 33, No. 5, 01.05.2006, p. 368-379.

Research output: Contribution to journalArticle

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