Effect of various implant coatings on biological responses in MG63 using cDNA microarray

C. S. Kim, S. H. Sohn, S. K. Jeon, K. N. Kim, J. J. Ryu, M. K. Kim

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    43 Citations (Scopus)

    Abstract

    During the process of bone formation, titanium (Ti) surface is an important factor in the modulation of osteoblastic function. This study was conducted in order to determine the effects of different Ti surfaces on the biological responses of a human osteoblast-like cell line (MG63). MG63 cells were cultured on smooth (S), sandblasted large-grit and acid etching (SLA), hydroxyapatite (HA), hydroxyfluoride (HF), titanium nitrate (TIN), and diamond-like carbon (DLC) Ti. The morphology of these cells were assessed by SEM. The cDNAs prepared from the total RNAs of the MG63 were hybridized into a human cDNA microarray (1152 elements). The appearances of the surfaces observed by SEM were different on each of the six dental substrate types. The SLA and HA surfaces were determined to be rougher than the others. MG63 cells cultured on SLA and HA exhibited cell-matrix interactions. In the expression of genes involved in osseointegration, several genes, including bone morphogenetic protein, cadherin, integrin, and insulin-like growth factors, were upregulated on the different surfaces. Several genes, including fibroblast growth factor receptor 4, Bcl 2-related protein, and collagen, were downregulated on the different surfaces. The attachment and expression of key osteogenic regulatory genes were enhanced by the surface roughness of the dental materials used.

    Original languageEnglish
    Pages (from-to)368-379
    Number of pages12
    JournalJournal of Oral Rehabilitation
    Volume33
    Issue number5
    DOIs
    Publication statusPublished - 2006 May

    Keywords

    • Cell morphology
    • Dental titanium surfaces
    • Expression profiling
    • Gene expression
    • Gene profiling
    • cDNA microarray

    ASJC Scopus subject areas

    • Dentistry(all)

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