Effective antifouling using quorum-quenching acylase stabilized in magnetically-separable mesoporous silica

Byoungsoo Lee, Kyung Min Yeon, Jongmin Shim, Sang Ryoung Kim, Chung Hak Lee, Jinwoo Lee, Jungbae Kim

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Highly effective antifouling was achieved by immobilizing and stabilizing an acylase, disrupting bacterial cell-to-cell communication, in the form of cross-linked enzymes in magnetically separable mesoporous silica. This so-called "quorum-quenching" acylase (AC) was adsorbed into spherical mesoporous silica (S-MPS) with magnetic nanoparticles (Mag-S-MPS), and further cross-linked for the preparation of nanoscale enzyme reactors of AC in Mag-S-MPS (NER-AC/Mag-S-MPS). NER-AC effectively stabilized the AC activity under rigorous shaking at 200 rpm for 1 month, while free and adsorbed AC lost more than 90% of their initial activities in the same condition within 1 and 10 days, respectively. When applied to the membrane filtration for advanced water treatment, NER-AC efficiently alleviated the biofilm maturation of Pseudomonas aeruginosa PAO1 on the membrane surface, thereby enhancing the filtration performance by preventing membrane fouling. Highly stable and magnetically separable NER-AC, as an effective and sustainable antifouling material, has a great potential to be used in the membrane filtration for water reclamation.

Original languageEnglish
Pages (from-to)1153-1159
Number of pages7
JournalBiomacromolecules
Volume15
Issue number4
DOIs
Publication statusPublished - 2014 Apr 14

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amidase
Silicon Dioxide
Quenching
Silica
Membranes
Enzymes
Wastewater reclamation
Membrane fouling
Biofilms
Water treatment
Nanoparticles
Communication

ASJC Scopus subject areas

  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials
  • Bioengineering

Cite this

Effective antifouling using quorum-quenching acylase stabilized in magnetically-separable mesoporous silica. / Lee, Byoungsoo; Yeon, Kyung Min; Shim, Jongmin; Kim, Sang Ryoung; Lee, Chung Hak; Lee, Jinwoo; Kim, Jungbae.

In: Biomacromolecules, Vol. 15, No. 4, 14.04.2014, p. 1153-1159.

Research output: Contribution to journalArticle

Lee, Byoungsoo ; Yeon, Kyung Min ; Shim, Jongmin ; Kim, Sang Ryoung ; Lee, Chung Hak ; Lee, Jinwoo ; Kim, Jungbae. / Effective antifouling using quorum-quenching acylase stabilized in magnetically-separable mesoporous silica. In: Biomacromolecules. 2014 ; Vol. 15, No. 4. pp. 1153-1159.
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