Effective inactivation of Candida albicans biofilms by using supercritical carbon dioxide

Hyong S.eok Park, Jungwoo Yang, Hee J.ung Choi, Kyoung Heon Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Present sterilization methods for biofilms in medical devices have limitations. Therefore, an alternative sterilization method using supercritical carbon dioxide (SC-CO2) was tested on Candida albicans biofilms. The effect of varying pressure, temperature, and treatment time on the inactivation of C. albicans spores in suspensions and in biofilms was examined. The parameters such as treatment time, pressure, and temperature that led to the complete inactivation of C. albicans biofilms ranged 5-20 min, 100-200 bar, and 35-45 °C, respectively. Notably, treatment of SC-CO2 at either 100 bar and 40 °C or 200 bar and 30 °C induced complete inactivation of spores within 5 min. Furthermore, it was found that wet biofilms (0.4 %, w/w) had higher sensitivity to SC-CO2 than dried biofilms. Finally, spore inactivation was confirmed by confocal laser scanning microscopy. In this study, the use of a low-temperature SC-CO2 sterilization method was proven to be effective in fungal biofilm inactivation, and the moisture content of biofilms was revealed to be the key factor for biofilm inactivation.

Original languageEnglish
Pages (from-to)1731-1737
Number of pages7
JournalBioprocess and Biosystems Engineering
Volume38
Issue number9
DOIs
Publication statusPublished - 2015 Sep 1

Fingerprint

Candida
Biofilms
Candida albicans
Carbon Dioxide
Carbon dioxide
Spores
Temperature
Pressure
Confocal Microscopy
Suspensions
Microscopic examination
Moisture
Scanning
Equipment and Supplies
Lasers

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Effective inactivation of Candida albicans biofilms by using supercritical carbon dioxide. / Park, Hyong S.eok; Yang, Jungwoo; Choi, Hee J.ung; Kim, Kyoung Heon.

In: Bioprocess and Biosystems Engineering, Vol. 38, No. 9, 01.09.2015, p. 1731-1737.

Research output: Contribution to journalArticle

Park, Hyong S.eok ; Yang, Jungwoo ; Choi, Hee J.ung ; Kim, Kyoung Heon. / Effective inactivation of Candida albicans biofilms by using supercritical carbon dioxide. In: Bioprocess and Biosystems Engineering. 2015 ; Vol. 38, No. 9. pp. 1731-1737.
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