Effective production of microinjectable blastocysts for germ-line transmission of embryonic stem cells

Sun Uk Kim, Ying Hao Han, Tae Hoon Lee, Byung Hwa Hyun, Sang Ho Lee, Dong Seok Lee, Dae Yeul Yu

Research output: Contribution to journalArticle

Abstract

Blastocysts of C57BL/6 mice obtained either 2.5 or 3.5 days post-coitum (dpc) were examined for efficient microinjection after overnight in vitro culture. Incidences of zona-free embryos were much higher at 3.5 dpc after natural mating (1.05/mouse) and superovulation (2.83/mouse) than at 2.5 dpc after natural mating (0.05/mouse) and superovulation (0.01/mouse). By testing germ-line competency of gene-targeted J1 embryonic stem cells, superovulation and/or in vitro culture should be recommended for producing microinjectable blastocysts for production of high germ-line chimeras.

Original languageEnglish
Pages (from-to)475-477
Number of pages3
JournalExperimental Animals
Volume53
Issue number5
DOIs
Publication statusPublished - 2004

Keywords

  • C57BL/6 mice
  • Germ-line chimera
  • Host embryo

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

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