Effects and action mechanism of Diospyros kaki on the differentiation of human leukemia HL-60 cells

Seung Hyun Kim, Seung Sik Cho, Jaya Ram Simkhada, Sung Ju Park, Hyo Jeong Lee, Tae Sung Kim, Jin Cheol Yoo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Diospyros kaki Thunb. (Ebenaceae) is widely distributed in North-East Asian countries. Almost all parts of this plant have been traditionally used as medicine. Human promyelocytic leukemia cells differentiate into monocytes or granulocytes when treated with 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] or all-trans retinoic acid (ATRA). Combination of low doses of ATRA or 1,25-dihydroxyvitamin D3 that do not induce toxicity with another drug is a useful strategy for acute promyelocytic leukemia therapy. Our main aim was to investigate the effect of an acetone extract of D. kaki leaves (KV-1) on HL-60 cell differentiation in combination of ATRA or 1,25-dihydroxyvitamin D3. Treatment of HL-60 cells with zero to 100 μg/ml of KV-1 for 72 h induced a small increase in cell differentiation. Surprisingly, a synergistic induction of differentiation was observed when the HL-60 cells were treated with ATRA or 1,25-(OH) 2D3 and the extract. The inhibitors of protein kinase C (PKC) (α and ßI) and extracellular signal-regulated kinase (ERK), but not of phosphoinositide 3-kinase (PI3-K) and c-Jun N-terminal kinase (JNK) inhibited the HL-60 differentiation induced by the extract in combination of ATRA or 1,25-(OH)2D3, suggesting that PKC and ERK were involved in the cell differentiation enhancement by the extract. The results indicate that the acetone extract of D. kaki leaves has the ability to enhance HL-60 cell differentiation and suggest that it may be useful in acute promyelocytic leukemia therapy.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalOncology Reports
Volume23
Issue number1
DOIs
Publication statusPublished - 2010 Feb 11

Fingerprint

Diospyros
HL-60 Cells
Tretinoin
Leukemia
Calcitriol
Cell Differentiation
Acute Promyelocytic Leukemia
Extracellular Signal-Regulated MAP Kinases
Acetone
Protein Kinase C
Ebenaceae
1-Phosphatidylinositol 4-Kinase
JNK Mitogen-Activated Protein Kinases
Granulocytes
Monocytes
Medicine
Therapeutics
Pharmaceutical Preparations

Keywords

  • 1,25-dihydroxyvitamin d
  • All-trans retinoic acid
  • Cell differentiation
  • Diospyros kaki
  • Hl-60 cells
  • Protein kinase C

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Effects and action mechanism of Diospyros kaki on the differentiation of human leukemia HL-60 cells. / Kim, Seung Hyun; Cho, Seung Sik; Simkhada, Jaya Ram; Park, Sung Ju; Lee, Hyo Jeong; Kim, Tae Sung; Yoo, Jin Cheol.

In: Oncology Reports, Vol. 23, No. 1, 11.02.2010, p. 89-95.

Research output: Contribution to journalArticle

Kim, Seung Hyun ; Cho, Seung Sik ; Simkhada, Jaya Ram ; Park, Sung Ju ; Lee, Hyo Jeong ; Kim, Tae Sung ; Yoo, Jin Cheol. / Effects and action mechanism of Diospyros kaki on the differentiation of human leukemia HL-60 cells. In: Oncology Reports. 2010 ; Vol. 23, No. 1. pp. 89-95.
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