Effects of 20-hydroxyecdysone and serotonin on neurite growth and survival rate of antennal lobe neurons in pupal stage of the silk moth Bombyx mori in vitro

Hun Hee Park, Cheolin Park, Kwan Seon Kim, Oh Seok Kwon, Sung Sik Han, Jae Sam Hwang, Sang Mong Lee, Su Il Seong, Seok Woo Kang, Hak Ryul Kim, Bong Hee Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Effects of 20-hydroxyecdysone and serotonin on the morphological development and the survival of antennal lobe neurons from day-2 pupal brains of the silk moth Bombyx mori were investigated in vitro. Four morphologically distinct neuronal types could be identified in the cultured antennal lobe neurons: unipolar, bipolar, multi-polar and projection neurons. Antennal lobe neurons in culture with 20-hydroxyecdysone and serotonin showed different patterns of the morphological development from those described in Manduca sexta. Projection neurons extend their neurites remarkably by 20-hydroxyecdysone in B. mori, but there is no extension from antennal lobe neurons in M. sexta. Multi-polar neurons conspicuously increase only formation of new branches from their primary neurites by serotonin in B. mori, but there are both extension and branching of the neurites in M. sexta. On day-5, antennal lobe neurons in lower titers of 20-hydroxyecdysone had significantly higher survival rates than those in higher titers. Neurons cultured for 7 days at different levels of 20-hydroxyecdysone generally showed significantly lower survival rates than neurons cultured for 5 days under the same conditions.

Original languageEnglish
Pages (from-to)111-119
Number of pages9
JournalZoological Science
Volume20
Issue number2
DOIs
Publication statusPublished - 2003 Feb 1

Fingerprint

Ecdysterone
antennal lobe
neurites
Bombyx
Silk
ecdysterone
Moths
Neurites
Bombyx mori
silk
serotonin
moths
Serotonin
survival rate
neurons
Neurons
Growth
Manduca
Manduca sexta
In Vitro Techniques

Keywords

  • 20-hydroxyecdysone
  • Neurite growth
  • Neuron culture
  • Serotonin
  • Silk moth

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Effects of 20-hydroxyecdysone and serotonin on neurite growth and survival rate of antennal lobe neurons in pupal stage of the silk moth Bombyx mori in vitro. / Park, Hun Hee; Park, Cheolin; Kim, Kwan Seon; Kwon, Oh Seok; Han, Sung Sik; Hwang, Jae Sam; Lee, Sang Mong; Seong, Su Il; Kang, Seok Woo; Kim, Hak Ryul; Lee, Bong Hee.

In: Zoological Science, Vol. 20, No. 2, 01.02.2003, p. 111-119.

Research output: Contribution to journalArticle

Park, Hun Hee ; Park, Cheolin ; Kim, Kwan Seon ; Kwon, Oh Seok ; Han, Sung Sik ; Hwang, Jae Sam ; Lee, Sang Mong ; Seong, Su Il ; Kang, Seok Woo ; Kim, Hak Ryul ; Lee, Bong Hee. / Effects of 20-hydroxyecdysone and serotonin on neurite growth and survival rate of antennal lobe neurons in pupal stage of the silk moth Bombyx mori in vitro. In: Zoological Science. 2003 ; Vol. 20, No. 2. pp. 111-119.
@article{67a3f2f96cb14d9290b5ef87e9e4d73c,
title = "Effects of 20-hydroxyecdysone and serotonin on neurite growth and survival rate of antennal lobe neurons in pupal stage of the silk moth Bombyx mori in vitro",
abstract = "Effects of 20-hydroxyecdysone and serotonin on the morphological development and the survival of antennal lobe neurons from day-2 pupal brains of the silk moth Bombyx mori were investigated in vitro. Four morphologically distinct neuronal types could be identified in the cultured antennal lobe neurons: unipolar, bipolar, multi-polar and projection neurons. Antennal lobe neurons in culture with 20-hydroxyecdysone and serotonin showed different patterns of the morphological development from those described in Manduca sexta. Projection neurons extend their neurites remarkably by 20-hydroxyecdysone in B. mori, but there is no extension from antennal lobe neurons in M. sexta. Multi-polar neurons conspicuously increase only formation of new branches from their primary neurites by serotonin in B. mori, but there are both extension and branching of the neurites in M. sexta. On day-5, antennal lobe neurons in lower titers of 20-hydroxyecdysone had significantly higher survival rates than those in higher titers. Neurons cultured for 7 days at different levels of 20-hydroxyecdysone generally showed significantly lower survival rates than neurons cultured for 5 days under the same conditions.",
keywords = "20-hydroxyecdysone, Neurite growth, Neuron culture, Serotonin, Silk moth",
author = "Park, {Hun Hee} and Cheolin Park and Kim, {Kwan Seon} and Kwon, {Oh Seok} and Han, {Sung Sik} and Hwang, {Jae Sam} and Lee, {Sang Mong} and Seong, {Su Il} and Kang, {Seok Woo} and Kim, {Hak Ryul} and Lee, {Bong Hee}",
year = "2003",
month = "2",
day = "1",
doi = "10.2108/zsj.20.111",
language = "English",
volume = "20",
pages = "111--119",
journal = "Zoological Science",
issn = "0289-0003",
publisher = "Zoological Society of Japan",
number = "2",

}

TY - JOUR

T1 - Effects of 20-hydroxyecdysone and serotonin on neurite growth and survival rate of antennal lobe neurons in pupal stage of the silk moth Bombyx mori in vitro

AU - Park, Hun Hee

AU - Park, Cheolin

AU - Kim, Kwan Seon

AU - Kwon, Oh Seok

AU - Han, Sung Sik

AU - Hwang, Jae Sam

AU - Lee, Sang Mong

AU - Seong, Su Il

AU - Kang, Seok Woo

AU - Kim, Hak Ryul

AU - Lee, Bong Hee

PY - 2003/2/1

Y1 - 2003/2/1

N2 - Effects of 20-hydroxyecdysone and serotonin on the morphological development and the survival of antennal lobe neurons from day-2 pupal brains of the silk moth Bombyx mori were investigated in vitro. Four morphologically distinct neuronal types could be identified in the cultured antennal lobe neurons: unipolar, bipolar, multi-polar and projection neurons. Antennal lobe neurons in culture with 20-hydroxyecdysone and serotonin showed different patterns of the morphological development from those described in Manduca sexta. Projection neurons extend their neurites remarkably by 20-hydroxyecdysone in B. mori, but there is no extension from antennal lobe neurons in M. sexta. Multi-polar neurons conspicuously increase only formation of new branches from their primary neurites by serotonin in B. mori, but there are both extension and branching of the neurites in M. sexta. On day-5, antennal lobe neurons in lower titers of 20-hydroxyecdysone had significantly higher survival rates than those in higher titers. Neurons cultured for 7 days at different levels of 20-hydroxyecdysone generally showed significantly lower survival rates than neurons cultured for 5 days under the same conditions.

AB - Effects of 20-hydroxyecdysone and serotonin on the morphological development and the survival of antennal lobe neurons from day-2 pupal brains of the silk moth Bombyx mori were investigated in vitro. Four morphologically distinct neuronal types could be identified in the cultured antennal lobe neurons: unipolar, bipolar, multi-polar and projection neurons. Antennal lobe neurons in culture with 20-hydroxyecdysone and serotonin showed different patterns of the morphological development from those described in Manduca sexta. Projection neurons extend their neurites remarkably by 20-hydroxyecdysone in B. mori, but there is no extension from antennal lobe neurons in M. sexta. Multi-polar neurons conspicuously increase only formation of new branches from their primary neurites by serotonin in B. mori, but there are both extension and branching of the neurites in M. sexta. On day-5, antennal lobe neurons in lower titers of 20-hydroxyecdysone had significantly higher survival rates than those in higher titers. Neurons cultured for 7 days at different levels of 20-hydroxyecdysone generally showed significantly lower survival rates than neurons cultured for 5 days under the same conditions.

KW - 20-hydroxyecdysone

KW - Neurite growth

KW - Neuron culture

KW - Serotonin

KW - Silk moth

UR - http://www.scopus.com/inward/record.url?scp=0037768242&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037768242&partnerID=8YFLogxK

U2 - 10.2108/zsj.20.111

DO - 10.2108/zsj.20.111

M3 - Article

VL - 20

SP - 111

EP - 119

JO - Zoological Science

JF - Zoological Science

SN - 0289-0003

IS - 2

ER -