Effects of 20-hydroxyecdysone and serotonin on neurite growth and survival rate of antennal lobe neurons in pupal stage of the silk moth Bombyx mori in vitro

Hun Hee Park, Cheolin Park, Kwan Seon Kim, Oh Seok Kwon, Sung Sik Han, Jae Sam Hwang, Sang Mong Lee, Su Il Seong, Seok Woo Kang, Hak Ryul Kim, Bong Hee Lee

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9 Citations (Scopus)

Abstract

Effects of 20-hydroxyecdysone and serotonin on the morphological development and the survival of antennal lobe neurons from day-2 pupal brains of the silk moth Bombyx mori were investigated in vitro. Four morphologically distinct neuronal types could be identified in the cultured antennal lobe neurons: unipolar, bipolar, multi-polar and projection neurons. Antennal lobe neurons in culture with 20-hydroxyecdysone and serotonin showed different patterns of the morphological development from those described in Manduca sexta. Projection neurons extend their neurites remarkably by 20-hydroxyecdysone in B. mori, but there is no extension from antennal lobe neurons in M. sexta. Multi-polar neurons conspicuously increase only formation of new branches from their primary neurites by serotonin in B. mori, but there are both extension and branching of the neurites in M. sexta. On day-5, antennal lobe neurons in lower titers of 20-hydroxyecdysone had significantly higher survival rates than those in higher titers. Neurons cultured for 7 days at different levels of 20-hydroxyecdysone generally showed significantly lower survival rates than neurons cultured for 5 days under the same conditions.

Original languageEnglish
Pages (from-to)111-119
Number of pages9
JournalZoological Science
Volume20
Issue number2
DOIs
Publication statusPublished - 2003 Feb 1

Keywords

  • 20-hydroxyecdysone
  • Neurite growth
  • Neuron culture
  • Serotonin
  • Silk moth

ASJC Scopus subject areas

  • Animal Science and Zoology

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    Park, H. H., Park, C., Kim, K. S., Kwon, O. S., Han, S. S., Hwang, J. S., Lee, S. M., Seong, S. I., Kang, S. W., Kim, H. R., & Lee, B. H. (2003). Effects of 20-hydroxyecdysone and serotonin on neurite growth and survival rate of antennal lobe neurons in pupal stage of the silk moth Bombyx mori in vitro. Zoological Science, 20(2), 111-119. https://doi.org/10.2108/zsj.20.111