Effects of a hemagglutinin D222G substitution on the pathogenicity of 2009 influenza A (H1N1) virus in mice

Jin Il Kim, Ilseob Lee, Sehee Park, Sangmoo Lee, Min Woong Hwang, Joon Yong Bae, Jun Heo, Donghwan Kim, Seok Il Jang, Jin Won Song, Man-Seong Park

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Abstract

The surface glycoprotein hemagglutinin (HA) of influenza virus initiates the infection process by binding to sialic acid receptors on upper respiratory cells in the host. In contrast to avian influenza viruses, which bind to sialic acids connected by an α2-3 linkage to the penultimate galactose, human influenza viruses prefer sialic acids with an α2-6 linkage. Recently, there have been multiple cases of severe human infections associated with an HA D222G mutant influenza virus. In this study, we have investigated the pathogenic effects of the HA D222G substitution in a 2009 pandemic H1N1 virus in mice. Compared with the A/Korea/01/2009 (K/09) virus, the HA D222G mutant showed reduced growth in cells and reduced binding avidity to human and turkey red blood cells. In a BALB/c mouse infection model, infection with the HA D222G mutant virus resulted in less body weight loss when compared to the parental K/09 virus. Altogether, our data suggest that the HA D222G substitution in the K/09 virus might be deleterious to viral fitness.

Original languageEnglish
Pages (from-to)2559-2565
Number of pages7
JournalArchives of Virology
Volume159
Issue number10
DOIs
Publication statusPublished - 2014 Jan 1

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Hemagglutinins
Viruses
Substitution reactions
Sialic Acids
Membrane Glycoproteins
Galactose
Blood
Cells

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Effects of a hemagglutinin D222G substitution on the pathogenicity of 2009 influenza A (H1N1) virus in mice. / Kim, Jin Il; Lee, Ilseob; Park, Sehee; Lee, Sangmoo; Hwang, Min Woong; Bae, Joon Yong; Heo, Jun; Kim, Donghwan; Jang, Seok Il; Song, Jin Won; Park, Man-Seong.

In: Archives of Virology, Vol. 159, No. 10, 01.01.2014, p. 2559-2565.

Research output: Contribution to journalArticle

Kim, Jin Il ; Lee, Ilseob ; Park, Sehee ; Lee, Sangmoo ; Hwang, Min Woong ; Bae, Joon Yong ; Heo, Jun ; Kim, Donghwan ; Jang, Seok Il ; Song, Jin Won ; Park, Man-Seong. / Effects of a hemagglutinin D222G substitution on the pathogenicity of 2009 influenza A (H1N1) virus in mice. In: Archives of Virology. 2014 ; Vol. 159, No. 10. pp. 2559-2565.
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