Effects of acute oral administration of vitamin C on the mouse liver transcriptome

Hee Jin Jun, Sukyung Kim, Kevin Dawson, DalWoong Choi, Jong Sang Kim, Raymond L. Rodriguez, Sung-Joon Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Vitamin C is a strong antioxidant that alters gene expression in cells, and its effects can be modified by cellular oxidative stress. We investigated the genome-wide effects of vitamin C on the in vivo transcriptome in the liver, which synthesizes various enzymes and proteins to defend against cellular oxidative stress. We fed mice vitamin C (0.056 mg/g of body weight) for 1 week and performed DNA microarray analysis with hepatic mRNA in fasting and refeeding states to mimic physiological conditions of oxidative stress. Significance analysis of microarray data identified approximately 6, 000 genes differentially expressed in both fasting and refeeding states. In the fasting state, vitamin C induced overall energy metabolism as well as radical scavenging pathways. These were ameliorated in the refeeding state. These findings suggest that vitamin C has profound and immediate global effects on hepatic gene expression, which may help prevent oxidative stress, and that long-term treatment with vitamin C might reduce the risk of chronic disease.

Original languageEnglish
Pages (from-to)181-194
Number of pages14
JournalJournal of Medicinal Food
Volume14
Issue number3
DOIs
Publication statusPublished - 2011 Mar 1

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Transcriptome
Ascorbic Acid
Oral Administration
Liver
Oxidative Stress
Fasting
Microarray Analysis
Gene Expression
Oligonucleotide Array Sequence Analysis
Energy Metabolism
Chronic Disease
Antioxidants
Body Weight
Genome
Messenger RNA
Enzymes
Genes
Proteins

Keywords

  • antioxidant activity
  • liver
  • microarray
  • nutrigenomics
  • real-time polymerase chain reaction
  • vitamin C

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Effects of acute oral administration of vitamin C on the mouse liver transcriptome. / Jun, Hee Jin; Kim, Sukyung; Dawson, Kevin; Choi, DalWoong; Kim, Jong Sang; Rodriguez, Raymond L.; Lee, Sung-Joon.

In: Journal of Medicinal Food, Vol. 14, No. 3, 01.03.2011, p. 181-194.

Research output: Contribution to journalArticle

Jun, Hee Jin ; Kim, Sukyung ; Dawson, Kevin ; Choi, DalWoong ; Kim, Jong Sang ; Rodriguez, Raymond L. ; Lee, Sung-Joon. / Effects of acute oral administration of vitamin C on the mouse liver transcriptome. In: Journal of Medicinal Food. 2011 ; Vol. 14, No. 3. pp. 181-194.
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