Effects of anodized titanium with Arg-Gly-Asp (RGD) peptide immobilized via chemical grafting or physical adsorption on bone cell adhesion and differentiation

Jae Jun Ryu, Kyeongsoon Park, Hyo Sop Kim, Chang Mo Jeong, Jung Bo Huh

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Purpose: This study examined the effects of the immobilization of Arg-Gly-Asp (RGD) peptide (CAAALLLKERGDSK) on anodized titanium (Ti) via chemical grafting or physical adsorption methods on cell adhesion and osteoblast differentiation. Materials and Methods: The RGD peptide was immobilized on the anodized Ti surface by means of physical adsorption or chemical grafting. The chemical composition of each RGD-immobilized Ti substrate was examined by x-ray photoelectron spectroscopy. The level of cell proliferation was investigated via tetrazolium (XTT) assay. Alkaline phosphatase activity and calcium deposition were evaluated by alizarin red S staining, and mRNA expression of the differentiated osteoblast marker genes was analyzed by reverse-transcriptase polymerase chain reaction. Results: Cell adhesion was enhanced on the RGD-immobilized Ti substrates compared to the anodized Ti surfaces. In addition, significantly increased cell spreading and proliferation were observed with the cells grown on the RGD-immobilized Ti (P < .05). Furthermore, the osteoblasts on the RGD-immobilized Ti showed significant increases in the integrin β1 and type I collagen levels and small increases in osteonectin and osteocalcin levels (P < .05). Interestingly, the chemical grafting method resulted in significantly greater effects on adhesion and differentiation than the physical adsorption method (P < .05). Conclusion: RGD-immobilized Ti substrates might be effective in improving the osseointegration of dental implants. In particular, the chemical grafting method of RGD immobilization is more favorable and is expected to provide positive outcomes with future animal and clinical studies.

Original languageEnglish
Pages (from-to)963-972
Number of pages10
JournalInternational Journal of Oral and Maxillofacial Implants
Volume28
Issue number4
DOIs
Publication statusPublished - 2013

Keywords

  • Anodized titanium
  • Cell adhesion
  • Differentiation
  • Immobilization
  • RGD peptide

ASJC Scopus subject areas

  • Oral Surgery

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