Effects of autologous platelet-rich plasma on regeneration of damaged endometrium in female rats

Hang Yong Jang, Soo Min Myoung, Jeong Min Choe, Tak Kim, Yong Pil Cheon, Yong Min Kim, Hyun-Tae Park

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose: To investigate whether autologous platelet-rich plasma (PRP) treatment can improve regeneration of the endometrium in an experimental model of ethanol-induced damage. Materials and Methods: Sixty female Sprague-Dawley rats were randomly assigned into three groups: control group, ethanol group, and PRP-treated group (administration of 0.25 mL of PRP into both uterine cavities 72 hours after ethanol injection). After 15 days of endometrial damage, all the animals were sacrificed during the estrous cycle, and samples were taken from the mid-uterine horn. Functional and structural recovery of the endometrium was analyzed by hematoxylin-eosin (H&E) and Masson trichrome (MT) staining, real-time polymerase chain reaction (PCR) assay, and immuno-histochemical (IHC) analyses. Results: H&E and MT staining confirmed significantly decreased fibrosis and increased cellular proliferation in the PRP-treated group, compared to the ethanol group. The endometrial areas in the ethanol and PRP-treated groups were 212.83±15.84 µm2 and 262.34±12.33 µm2 (p=0.065). Significantly stronger IHC expression of cytokeratin, homeobox A10 (HOXA10), vascular endothelial growth factor (VEGF), and Ki-67 was found in the PRP-treated group, compared to the ethanol group. In real-time PCR analyses, interleukin-1β mRNA was down-regulated, while c-Kit mRNA was up-regulated, in the PRP-treated group, compared to the ethanol group. Conclusion: Intrauterine administration of autologous PRP stimulated and accelerated regeneration of the endometrium and also decreased fibrosis in a murine model of damaged endometrium.

Original languageEnglish
Pages (from-to)1195-1203
Number of pages9
JournalYonsei Medical Journal
Volume58
Issue number6
DOIs
Publication statusPublished - 2017 Nov 1

Fingerprint

Platelet-Rich Plasma
Endometrium
Regeneration
Ethanol
Real-Time Polymerase Chain Reaction
Fibrosis
Staining and Labeling
Messenger RNA
Estrous Cycle
Homeobox Genes
antineoplaston A10
Hematoxylin
Eosine Yellowish-(YS)
Keratins
Interleukin-1
Vascular Endothelial Growth Factor A
Sprague Dawley Rats
Theoretical Models
Cell Proliferation
Control Groups

Keywords

  • Fibrosis
  • Platelet-rich plasma (PRP)
  • Regeneration
  • Thin endometrium

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effects of autologous platelet-rich plasma on regeneration of damaged endometrium in female rats. / Jang, Hang Yong; Myoung, Soo Min; Choe, Jeong Min; Kim, Tak; Cheon, Yong Pil; Kim, Yong Min; Park, Hyun-Tae.

In: Yonsei Medical Journal, Vol. 58, No. 6, 01.11.2017, p. 1195-1203.

Research output: Contribution to journalArticle

Jang, Hang Yong ; Myoung, Soo Min ; Choe, Jeong Min ; Kim, Tak ; Cheon, Yong Pil ; Kim, Yong Min ; Park, Hyun-Tae. / Effects of autologous platelet-rich plasma on regeneration of damaged endometrium in female rats. In: Yonsei Medical Journal. 2017 ; Vol. 58, No. 6. pp. 1195-1203.
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