Effects of benzo(a)pyrene on protein expression in Jurkat T-cells

Sangnam Oh, Hosub Im, Eunha Oh, Joohyun Lee, Jin Young Khim, Joohee Mun, Yanghee Kim, Eun Il Lee, Joon Kim, Dong Geun Sul

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants of air, water and soil, and are produced by the incomplete combustion of organic materials. The International Agency for Research on Cancer has characterized PAHs as carcinogens. In this study, we investigated the effects of benzo(a)pyrene (B(a)P), which is the most carcinogenic member of the PAHs, on Jurkat cell protein by proteomic analysis. Jurkat cells were treated with various concentrations of B(a)P (0, 2.5, 5, 10, 20 or 40 μM) for 24 or 48 h and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium and lactate dehydrogenase assays were carried out to determine cytotoxicity and a Comet assay was used to determinate genotoxicity. The cytotoxicity assays showed that 2.5 μM of B(a)P was the maximal concentration that did not cause any toxicity, but nevertheless, at this level B(a)P produced significant DNA damage in Jurkat cells at 48 h. Proteomic analysis using three different p/ ranges and large two-dimensional gel electrophoresis showed 3427 protein spots. A total of 46 (13 up- and 33 down-regulated) proteins were identified as biomarkers of B(a)P and showed dose-dependent expressions in Jurkat T-cell line exposed to B(a)P. Of these, 27 protein spots were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Two functionally differentiated protein groups were found. The protein group involving apoptosis and tumor suppression were found to be up-regulated, and B(a)P down-regulated enzyme was involved in energy metabolism, DNA synthesis and in cell structure and motility.

Original languageEnglish
Pages (from-to)3514-3526
Number of pages13
JournalProteomics
Volume4
Issue number11
DOIs
Publication statusPublished - 2004 Nov 1

Fingerprint

Jurkat Cells
T-cells
Benzo(a)pyrene
T-Lymphocytes
Polycyclic Aromatic Hydrocarbons
Assays
Proteins
Cytotoxicity
Proteomics
International Agencies
Air Pollutants
Comet Assay
DNA
Electrophoresis, Gel, Two-Dimensional
Biomarkers
Electrophoresis
L-Lactate Dehydrogenase
Carcinogens
Energy Metabolism
DNA Damage

Keywords

  • Benzo(a)pyrene
  • Jurkat cell
  • Matrix-assisted laser desorption/ionization-time of flight mass spectrometry
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Effects of benzo(a)pyrene on protein expression in Jurkat T-cells. / Oh, Sangnam; Im, Hosub; Oh, Eunha; Lee, Joohyun; Khim, Jin Young; Mun, Joohee; Kim, Yanghee; Lee, Eun Il; Kim, Joon; Sul, Dong Geun.

In: Proteomics, Vol. 4, No. 11, 01.11.2004, p. 3514-3526.

Research output: Contribution to journalArticle

Oh, S, Im, H, Oh, E, Lee, J, Khim, JY, Mun, J, Kim, Y, Lee, EI, Kim, J & Sul, DG 2004, 'Effects of benzo(a)pyrene on protein expression in Jurkat T-cells', Proteomics, vol. 4, no. 11, pp. 3514-3526. https://doi.org/10.1002/pmic.200400981
Oh S, Im H, Oh E, Lee J, Khim JY, Mun J et al. Effects of benzo(a)pyrene on protein expression in Jurkat T-cells. Proteomics. 2004 Nov 1;4(11):3514-3526. https://doi.org/10.1002/pmic.200400981
Oh, Sangnam ; Im, Hosub ; Oh, Eunha ; Lee, Joohyun ; Khim, Jin Young ; Mun, Joohee ; Kim, Yanghee ; Lee, Eun Il ; Kim, Joon ; Sul, Dong Geun. / Effects of benzo(a)pyrene on protein expression in Jurkat T-cells. In: Proteomics. 2004 ; Vol. 4, No. 11. pp. 3514-3526.
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