Effects of bone morphogenetic protein-7 (BMP-7) on primordial follicular growth in the mouse ovary

Woo Sik Lee, Se Jin Yoon, Tae Ki Yoon, Kwang Yul Cha, Sook Hwan Lee, Shunichi Shimasaki, Sang Ho Lee, Kyung Ah Lee

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Previously, bone morphogenetic protein-7 (BMP-7) was suggested as a factor that may act to facilitate the transition of follicles from primordial stage to the pool of developed primary, preantral, and antral follicles (Lee et al. 2001: Biol Reprod 65:994-999.). Thus, aim of the present study was to evaluate effect(s) of BMP-7 on the primordial-primary follicle transition. Neonatal mouse ovaries were cultured in the presence or absence of 100 mIU/ml FSH with various doses of BMP-7 (0, 10, and 100 ng/ml). After 4-day culture period, number of follicles was counted and the expression of transcripts for FSH receptor (FSHR), kit ligand (KL), and c-kit was measured by RT-PCR. BMP-7 alone at 100 ng/ml concentration stimulated follicle development with concurrent increase of mRNA for FSHR. BMP-7 alone down-regulated KL expression however, the ratio between KL1 and KL2 was increased. There was no change in the c-kit mRNA expression. Results of the present study suggest that the BMP-7 is one of the factors involved in primordial-primary follicle transition in the mouse ovary and it may play a role in expression of FSHR for further follicular development.

Original languageEnglish
Pages (from-to)159-163
Number of pages5
JournalMolecular Reproduction and Development
Volume69
Issue number2
DOIs
Publication statusPublished - 2004 Oct 1

Fingerprint

Bone Morphogenetic Protein 7
Ovary
FSH Receptors
Stem Cell Factor
Growth
Messenger RNA
Polymerase Chain Reaction

Keywords

  • BMP-7
  • C-kit
  • Kit ligand
  • Mouse
  • Primordial-primary follicle transition

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Lee, W. S., Yoon, S. J., Yoon, T. K., Cha, K. Y., Lee, S. H., Shimasaki, S., ... Lee, K. A. (2004). Effects of bone morphogenetic protein-7 (BMP-7) on primordial follicular growth in the mouse ovary. Molecular Reproduction and Development, 69(2), 159-163. https://doi.org/10.1002/mrd.20163

Effects of bone morphogenetic protein-7 (BMP-7) on primordial follicular growth in the mouse ovary. / Lee, Woo Sik; Yoon, Se Jin; Yoon, Tae Ki; Cha, Kwang Yul; Lee, Sook Hwan; Shimasaki, Shunichi; Lee, Sang Ho; Lee, Kyung Ah.

In: Molecular Reproduction and Development, Vol. 69, No. 2, 01.10.2004, p. 159-163.

Research output: Contribution to journalArticle

Lee, Woo Sik ; Yoon, Se Jin ; Yoon, Tae Ki ; Cha, Kwang Yul ; Lee, Sook Hwan ; Shimasaki, Shunichi ; Lee, Sang Ho ; Lee, Kyung Ah. / Effects of bone morphogenetic protein-7 (BMP-7) on primordial follicular growth in the mouse ovary. In: Molecular Reproduction and Development. 2004 ; Vol. 69, No. 2. pp. 159-163.
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